Artículo

Rosemblit, C.; Pozzi, A.G.; Ceballos, N.R. "Relationship between steroidogenesis and spermiation in Rana catesbeiana and Leptodactylus ocellatus" (2006) Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology. 176(6):559-566
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Abstract:

The present study employs an in vitro system to analyse the role of steroid hormones in hCG-induced spermiation in two species of anuran amphibian: Rana catesbeiana and Leptodactylus ocellatus. In vitro spermiation was induced with 10 IU hCG and the effect of different steroid-biosynthesis inhibitors was analysed. Cyanoketone (10-5 M), an inhibitor of 3-oxo-4-ene steroid biosynthesis, did not block hCG-inducing activity even when biosynthesis of androgen was significantly reduced. These results clearly showed that, in both species, spermiation-inducing action of hCG does not depend on the biosynthesis of 3-oxo-4-ene steroids. Moreover, when combined inhibitors, aminoglutethimide (10-5 M) plus cyanoketone (10-5 M), were employed, spermiation evoked by hCG was not modified while hCG-induced androgen secretion significantly decreased. Additionally, none of the steroids used, progesterone, 17, 20α-dihydroxy-4-pregnen-3-one, testosterone and 5α- dihydrotestosterone, were able to induce spermiation in the absence of hCG, confirming that steroids are not involved in that process. In conclusion, as previously described in Bufo arenarum, in L. ocellatus and R. catesbeiana hCG-induced spermiation does not depend on steroid biosynthesis. © Springer-Verlag 2006.

Registro:

Documento: Artículo
Título:Relationship between steroidogenesis and spermiation in Rana catesbeiana and Leptodactylus ocellatus
Autor:Rosemblit, C.; Pozzi, A.G.; Ceballos, N.R.
Filiación:Laboratorio de Endocrinología Comparada and PRHOM-CONICET, Departamento de Biodiversidad Y Biología Experimental, Pabellón 2, C1428EHA, Buenos Aires, Argentina
Palabras clave:Amphibians; Anura; hCG; Spermiation; Steroids; 17,20 dihydroxy 4 pregnen 3 one; 17,20-dihydroxy-4-pregnen-3-one; androgen; androstanolone; chorionic gonadotropin; progesterone; progesterone derivative; steroid; testosterone; animal; article; biosynthesis; frog; frogs and toads; human; male; physiology; secretion; spermatogenesis; Androgens; Animals; Anura; Chorionic Gonadotropin; Dihydrotestosterone; Humans; Hydroxyprogesterones; Male; Progesterone; Rana catesbeiana; Spermatogenesis; Steroids; Testosterone; Amphibia; Anura; Bufo arenarum; Leptodactylus ocellatus; Rana catesbeiana
Año:2006
Volumen:176
Número:6
Página de inicio:559
Página de fin:566
DOI: http://dx.doi.org/10.1007/s00360-006-0078-7
Título revista:Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
Título revista abreviado:J. Comp. Physiol. B Biochem. Syst. Environ. Physiol.
ISSN:01741578
CODEN:JCPBD
CAS:androstanolone, 521-18-6; chorionic gonadotropin, 9002-61-3; progesterone, 57-83-0; testosterone, 58-22-0; 17,20-dihydroxy-4-pregnen-3-one, 10456-50-5; Androgens; Chorionic Gonadotropin; Dihydrotestosterone, 521-18-6; Hydroxyprogesterones; Progesterone, 57-83-0; Steroids; Testosterone, 58-22-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01741578_v176_n6_p559_Rosemblit

Referencias:

  • Blair, A.P., Notes on anuran behavior, especially Rana catesbeiana (1963) Herpetologica, 19, p. 151
  • Callard, G.V., Autocrine and paracrine role of steroids during spermatogenesis: Studies in Squalus acanthias and Necturus maculosus (1992) J Exp Zool, 261, pp. 132-142
  • Callard, I.P., Callard, G.V., Lance, V., Bolaffi, J.L., Rosset, J.S., Testicular regulation in non-mammalian vertebrates (1978) Biol Reprod, 18, pp. 16-43
  • Canosa, L.F., Ceballos, N.R., Effects of different steroid-biosynthesis inhibitors on the testicular steroidogenesis of the toad Bufo arenarum (2001) J Comp Physiol B, 171, pp. 519-526
  • Canosa, L.F., Ceballos, N.R., Seasonal changes in testicular steroidogenesis in the toad Bufo arenarum H (2002) Gen Comp Endocrinol, 125, pp. 426-434
  • Canosa, L.F., Pozzi, A.G., Ceballos, N.R., Pregnenolone and progesterone metabolism by the testes of Bufo arenarum (1998) J Comp Physiol B, 168, pp. 491-496
  • Canosa, L.F., Pozzi, A.G., Rosemblit, C., Ceballos, N.R., Steroid production in toad (2003) J Steroid Biochem Mol Biol, 85, pp. 227-233
  • Delgado, M.J., Gutiérrez, P., Alonso-Bedate, M., Seasonal cycles in testicular activity in the frog, Rana perezi (1989) Gen Comp Endocrinol, 73, pp. 1-11
  • Dexter, R.N., Fishman, L.M., Ney, R.L., Liddle, G.W., Inhibition of adrenal corticosteroid synthesis by aminoglutethimide: Studies of the mechanism of action (1967) J Clin Endocrinol Metab, 27 (4), pp. 473-480
  • Emlen, S.T., Territoriality in the bullfrog, Rana catesbeiana (1968) Copeia, pp. 240-243
  • Gallardo, J.M., (1974) Anfibios de Los Alrededores de Buenos Aires, , Eudeba, Universidad de Buenos Aires, Buenos Aires
  • Guarino, F.M., Di Fiore, M.M., Caputo, V., Angelini, F., Iela, L., Rastogi, R.K., Seasonal analysis of the reproductive cycle in tow wild populations of Rana italica Dubois, 1985 (1993) Anim Biol, 2, pp. 25-43
  • Ishii, S., Itoh, M., Amplexus induces surge of luteinizing hormone in male toads, Bufo japonicus (1992) Gen Comp Endocrinol, 86, pp. 34-41
  • Itoh, M., Inoue, M., Ishii, S., Annual cycle of pituitary and plasma gonadotropins and sex steroids in a wild population of the toad, Bufo japonicus (1990) Gen Comp Endocrinol, 78, pp. 242-253
  • Kao, Y.H., Alexander, P.S., Yang, V.V.C., Yu, J.Y.L., Annual patterns of testicular development and activity in the Chinese bullfrog (Rana rugulosa Wiegmann) (1993) Zool Sci, 10, pp. 337-351
  • Kime, D.E., Hews, E.A., Androgen biosynthesis in vitro by testes from Amphibia (1978) Gen Comp Endocrinol, 35, pp. 280-288
  • Ko, S.K., Kang, H.M., Im, W.-B., Kwon, H.B., Testicular cycles in three species of Korean frogs: Rana nigromaculata, Rana rugosa, and Rana dybowskii (1998) Gen Comp Endocrinol, 111, pp. 347-358
  • Kobayashi, T., Sakai, N., Adachi, S., Asahina, K., Iwasawa, H., Nagahama, Y., 17α,20α-Dihydroxy-4-pregnen-3-one is the naturally occurring spermiation-inducing hormone in the testis of a frog, Rana nigromaculata (1993) Endocrinology, 133, pp. 321-327
  • Licht, P., Induction of spermiation in anurans by mammalian pituitary gonadotropins and their subunits (1973) Gen Comp Endocrinol, 20, pp. 522-529
  • Licht, P., McCreery, B.R., Barnes, R., Pang, R., Seasonal and stress related changes in plasma gonadotropins, sex steroids, and corticosterone in the bullfrog, Rana catesbeiana (1983) Gen Comp Endocrinol, 50, pp. 124-145
  • Lofts, B., Spermiation (1974) Physiology of the Amphibia, 2, pp. 157-161. , Lofts B (ed) Academic, New York
  • Nagahama, Y., Testis (1986) Vertebrates Endocrinology: Fundamentals and Biomedical Implications, 1, pp. 399-437. , Pang PKT, Schreibman MP, Gorbman A (eds) Academic, New York
  • Okada, Y., (1966) Fauna Japonica: Anura, , Biogeographical Society of Japan, Tokyo
  • Parvinen, M., Vihko, K.M., Toppari, J., Cell interactions during the seminiferous epithelial cycle (1986) Int Rev Cytol, 104, pp. 115-151
  • Pierantoni, R., Fasano, S., Di Mateo, L., Minucci, S., Varriale, B., Chieffi, G., Stimulatory effect of a GnRH agonist (buserelin) in in vivo and in vitro testosterone production by frog (Rana esculenta) testis (1984) Mol Cell Endocrinol, 38, pp. 215-219
  • Pierantoni, R., Cobellis, G., Meccariello, R., Palmiero, C., Fienga, G., Minucci, S., Fasano, S., The amphibian testis as model to study germ cell progression during spermatogenesis (2002) Comp Biochem Physiol B, 132, pp. 131-139
  • Pozzi, A.G., Ceballos, N.R., Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis (2000) Gen Comp Endocrinol, 119, pp. 164-171
  • Pudney, J., Leydig and Sertoli cells, nonmammalian (1998) Encyclopedia of Reproduction, 2, pp. 1008-1020. , Knobil E, Neill JD (eds) Academic, New York
  • Rastogi, R.K., Iela, L., Saxena, P.K., Chieffi, G., The control of spermatogenesis in the green frog, Rana esculenta (1976) J Exp Zool, 196, pp. 151-166
  • Rastogi, R.K., Iela, L., Delrio, G., Bagnara, J.T., Reproduction in the Mexican leaf frog, Pachymedusa dacnicolor II. The male (1986) Gen Comp Endocrinol, 62, pp. 23-35
  • Sasso-Cerri, E., De Faria, F.P., Freymüller, E., Miraglia, S.M., Testicular morphological changes during the seasonal reproductive cycle in the bullfrog Rana catesbeiana (2004) J Exp Zool, 301 A, pp. 249-260
  • Specker, J.L., Moore, F.L., Annual cycle of plasma androgens and testicular composition in the rough-skinned newt, Taricha granulosa (1980) Gen Comp Endocrinol, 42, pp. 297-303
  • Steel, R.G.D., Torrie, J.H., (1980) Principles and Procedures of Statistics. A Biomedical Approach, , Chapter 9. McGraw-Hill (ed), New York
  • Takahashi, M., Luu-The, V., Labrie, F., Inhibitory effect of synthetic progestins, 4MA and cyanoketone on human placental 3β-hydroxysteroid dehydrogenase/5-4-n-isomerase activity (1990) J Steroid Biochem Mol Biol, 37, pp. 231-236
  • Todo, T., Ikeuchi, T., Kobayashi, T., Kajiura-Kobayashi, H., Suzuki, K., Yoshikuni, M., Yamauchi, K., Nagahama, Y., Characterization of a testicular 17alpha, 20beta-dihydroxy-4-pregnen-3- one (a spermiation-inducing steroid in fish) receptor from a teleost, Japanese eel (Anguilla japonica) (2000) FEBS Lett, 465, pp. 12-17
  • Ueda, H., Kambegawa, A., Nagahama, Y., Involvement of gonadotrophin and steroid hormones in spermiation in the amago salmon, Oncorhynchus rhodurus, and goldfish, Carassius auratus (1985) Gen Comp Endocrinol, 59, pp. 24-30
  • Vizziano, D., Le Gac, F., Fostier, A., Effect of 17 beta-estradiol, testosterone, and 11-ketotestosterone on 17,20 beta-dihydroxy-4-pregnen-3-one production in the rainbow trout testis (1996) Gen Comp Endocrinol, 104, pp. 179-188
  • Wells, K.D., The social behaviour of anuran amphibians (1977) Anim Behav, 25, pp. 666-693
  • Wiewandt, T.A., Vocalization, aggressive behavior and territoriality in the bullfrog, Rana catesbeiana (1969) Copeia, pp. 276-285
  • Yueh, W.S., Chang, C.F., 17α,20β,21-trihydroxy-4-pregnen-3-one and 17α,20β- dihydroxy-4-pregnen-3-one stimulated spermiation in protandrous black porgy, Acanthopagrus schlegeli (1997) Fish Physiol Biochem, 17, pp. 187-193

Citas:

---------- APA ----------
Rosemblit, C., Pozzi, A.G. & Ceballos, N.R. (2006) . Relationship between steroidogenesis and spermiation in Rana catesbeiana and Leptodactylus ocellatus. Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, 176(6), 559-566.
http://dx.doi.org/10.1007/s00360-006-0078-7
---------- CHICAGO ----------
Rosemblit, C., Pozzi, A.G., Ceballos, N.R. "Relationship between steroidogenesis and spermiation in Rana catesbeiana and Leptodactylus ocellatus" . Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology 176, no. 6 (2006) : 559-566.
http://dx.doi.org/10.1007/s00360-006-0078-7
---------- MLA ----------
Rosemblit, C., Pozzi, A.G., Ceballos, N.R. "Relationship between steroidogenesis and spermiation in Rana catesbeiana and Leptodactylus ocellatus" . Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, vol. 176, no. 6, 2006, pp. 559-566.
http://dx.doi.org/10.1007/s00360-006-0078-7
---------- VANCOUVER ----------
Rosemblit, C., Pozzi, A.G., Ceballos, N.R. Relationship between steroidogenesis and spermiation in Rana catesbeiana and Leptodactylus ocellatus. J. Comp. Physiol. B Biochem. Syst. Environ. Physiol. 2006;176(6):559-566.
http://dx.doi.org/10.1007/s00360-006-0078-7