Abstract:
In Bufo arenarum, the biosynthesis of testosterone and 5α-dihydrotestosterone takes place through a complete 5-ene pathway, 5-androsten-3β,17β-diol being the immediate precursor of testosterone. Besides androgens, testes are able to synthesise 5α-pregnan-3,20-dione and several 3α and 20α reduced derivatives. During the breeding season, steroid biosynthesis turns from androgen to C21-steroid production. As a consequence, the cytochrome P450 17-hydroxylase, C17,20-lyase (CypP450c17) could be a key enzyme in that metabolic shift. The present study demonstrates that in testes of B. arenarum, CypP450c17 co-localises with glucose-6-phosphatase in the microsomal fraction. CypP450c17 possesses more affinity for pregnenolone than for progesterone in both non-reproductive (Km=43.764±4.63 nM and 2,170±630 nM, respectively) and reproductive (Km=37.46± 4.19 nM and 3,060±190 nM, respectively) seasons. These results could explain the predominance of the 5-ene pathway for testosterone biosynthesis. Toad CypP450c17 activity is higher in the non-reproductive period than the reproductive period, suggesting that this enzyme is an important factor in toad steroidogenic changes. Animals in reproductive conditions showed a significant reduction in circulating androgens. This is in agreement with the decrease in Vmax of cytochrome P450 17-hydroxylase activity, enhancing the physiological relevance of these in vitro results.
Registro:
Documento: |
Artículo
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Título: | Seasonal changes in the activity of cytochrome P450C17 from the testis of Bufo arenarum |
Autor: | Solari, J.J.F.; Pozzi, A.G.; Ceballos, N.R. |
Filiación: | PRHOM-CONICET/Lab. de Endocrinol. C., Depto. de Biodiversidad y Biol. Exp., Universidad de Buenos Aires, Buenos Aires, Argentina Depto. de Biodiversidad y Biol. Exp., Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
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Palabras clave: | Cytochrome P450C17; Enzyme activity; Kinetic parameters; Seasonal changes; Toad testis; antiserum; steroid 17alpha monooxygenase; testosterone; animal; article; blood; cell fractionation; kinetics; male; metabolism; season; testis; tissue distribution; toad; Animals; Bufo arenarum; Immune Sera; Kinetics; Male; Seasons; Steroid 17-alpha-Hydroxylase; Subcellular Fractions; Testis; Testosterone; Tissue Distribution |
Año: | 2002
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Volumen: | 172
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Número: | 8
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Página de inicio: | 685
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Página de fin: | 690
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DOI: |
http://dx.doi.org/10.1007/s00360-002-0296-6 |
Título revista: | Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
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Título revista abreviado: | J. Comp. Physiol. B Biochem. Syst. Environ. Physiol.
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ISSN: | 01741578
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CODEN: | JCPBD
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CAS: | steroid 17alpha monooxygenase, 9029-67-8; testosterone, 58-22-0; Immune Sera; Steroid 17-alpha-Hydroxylase, EC 1.14.99.9; Testosterone, 58-22-0
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01741578_v172_n8_p685_Solari |
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Citas:
---------- APA ----------
Solari, J.J.F., Pozzi, A.G. & Ceballos, N.R.
(2002)
. Seasonal changes in the activity of cytochrome P450C17 from the testis of Bufo arenarum. Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, 172(8), 685-690.
http://dx.doi.org/10.1007/s00360-002-0296-6---------- CHICAGO ----------
Solari, J.J.F., Pozzi, A.G., Ceballos, N.R.
"Seasonal changes in the activity of cytochrome P450C17 from the testis of Bufo arenarum"
. Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology 172, no. 8
(2002) : 685-690.
http://dx.doi.org/10.1007/s00360-002-0296-6---------- MLA ----------
Solari, J.J.F., Pozzi, A.G., Ceballos, N.R.
"Seasonal changes in the activity of cytochrome P450C17 from the testis of Bufo arenarum"
. Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, vol. 172, no. 8, 2002, pp. 685-690.
http://dx.doi.org/10.1007/s00360-002-0296-6---------- VANCOUVER ----------
Solari, J.J.F., Pozzi, A.G., Ceballos, N.R. Seasonal changes in the activity of cytochrome P450C17 from the testis of Bufo arenarum. J. Comp. Physiol. B Biochem. Syst. Environ. Physiol. 2002;172(8):685-690.
http://dx.doi.org/10.1007/s00360-002-0296-6