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Abstract:

Wild populations of Caiman latirostris are subject to sustainable use programs in Argentina, becoming a species with important impact in the regional economy, based in their skin and meat. Genetic studies are fundamental to acquire information on important parameters for conservation and management, which may be obtained from analysis of molecular markers. Some microsatellites have been previously isolated in this species, but due to some difficulties in using them, we obtained new ones using Next Generation Sequencing approach. This study reports eight new microsatellites for C. latirostris and tests their utility in a related species, Caiman yacare, with successful application in population genetics and mating systems studies. In addition, we shared data about a novel and fast bioinformatics tool to find microsatellites and to design their corresponding primers.

Registro:

Documento: Artículo
Título:Isolation and characterization of new microsatellite markers for application in population genetic studies of Caiman latirostris and related species
Autor:Larriera, A.; Siroski, P.A.; Rueda, E.C.; Saidman, B.O.; Amavet, P.S.
Filiación:Laboratorio de Genética, Departamento de Ciencias Naturales, Facultad de Humanidades y Ciencias, Santa Fe, 3000, Argentina
Proyecto Yacare - Laboratorio de Zoologia Aplicada: Anexo Vertebrados (FHUC - UNL/MASPyMA), A. del Valle 8700, Santa Fe, 3000, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, Buenos Aires, C1033AAJ, Argentina
Instituto de Ciencias Veterinarias Del Litoral (ICiVet-UNL-CONICET), Kreder 2805, Esperanza, S3080HOF, Argentina
Departamento Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina
Palabras clave:conservation genetics; crocodilians; SSRs; sustainable use; conservation genetics; crocodilian; genetic marker; meat; population genetics; regional economy; skin; sustainability; wild population; Argentina; Caiman latirostris; Caiman yacare; Crocodylidae (all crocodiles)
Año:2015
Volumen:36
Número:2
Página de inicio:175
Página de fin:180
DOI: http://dx.doi.org/10.1163/15685381-00002987
Título revista:Amphibia Reptilia
Título revista abreviado:Amphib. Reptilia
ISSN:01735373
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01735373_v36_n2_p175_Larriera

Referencias:

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Citas:

---------- APA ----------
Larriera, A., Siroski, P.A., Rueda, E.C., Saidman, B.O. & Amavet, P.S. (2015) . Isolation and characterization of new microsatellite markers for application in population genetic studies of Caiman latirostris and related species. Amphibia Reptilia, 36(2), 175-180.
http://dx.doi.org/10.1163/15685381-00002987
---------- CHICAGO ----------
Larriera, A., Siroski, P.A., Rueda, E.C., Saidman, B.O., Amavet, P.S. "Isolation and characterization of new microsatellite markers for application in population genetic studies of Caiman latirostris and related species" . Amphibia Reptilia 36, no. 2 (2015) : 175-180.
http://dx.doi.org/10.1163/15685381-00002987
---------- MLA ----------
Larriera, A., Siroski, P.A., Rueda, E.C., Saidman, B.O., Amavet, P.S. "Isolation and characterization of new microsatellite markers for application in population genetic studies of Caiman latirostris and related species" . Amphibia Reptilia, vol. 36, no. 2, 2015, pp. 175-180.
http://dx.doi.org/10.1163/15685381-00002987
---------- VANCOUVER ----------
Larriera, A., Siroski, P.A., Rueda, E.C., Saidman, B.O., Amavet, P.S. Isolation and characterization of new microsatellite markers for application in population genetic studies of Caiman latirostris and related species. Amphib. Reptilia. 2015;36(2):175-180.
http://dx.doi.org/10.1163/15685381-00002987