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Abstract:

OBJECTIVES: The aim of present paper was to study the probable role of glutamic acid (GLU) as a mediator of bisphenol A (BPA) effect at the hypothalamic level and its effects on the reproductive axis of prepubertal male rats. METHODS: Mated Wistar rats were treated with either 0.1% ethanol (control group, n=10) or BPA (BPA group, n=10) in their drinking water until their offspring were weaned at the age of 21 days. The estimated average dose of exposure to dams was approximately 2.5 mg/kg body weight/day of BPA. At the prepubertal stage (35 days of age), the male rats were sacrificed and Gn-RH and glutamic acid (GLU) release, an amino acid involved in Gn-RH secretion, were measured in hypothalamic samples containing medio basal and anterior preoptic area (MBH-APOA), by RIA and HPLC respectively. LH, FSH serum levels were measured by RIA and testosterone by EQLIA. RESULTS: Gn-RH and GLU release decreased significantly in animals exposed to BPA (p<0.001, p<0.01). LH, FSH and testosterone serum levels were also decreased by treatment (p<0.0001). CONCLUSION: Present results provide evidence that BPA may act at the hypothalamic level to decrease GLU release which in turn may modify Gn-RH secretion altering the normal function of the axis. © 2010 Neuroendocrinology Letters.

Registro:

Documento: Artículo
Título:Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats
Autor:Cardoso, N.; Pandolfi, M.; Ponzo, O.; Carbone, S.; Szwarcfarb, B.; Scacchi, P.; Reynoso, R.
Filiación:Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
CONICET, Buenos Aires, Argentina
Departamento de Biodiversidad Y Biología Experimental, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Bisphenol A; FSH; Glutamic acid; Gn-RH; LH; Prepubertal; 4,4' isopropylidenediphenol; follitropin; glutamic acid; gonadorelin; luteinizing hormone; testosterone; animal experiment; animal tissue; article; controlled study; female; hypothalamus; male; nonhuman; prepuberty; rat; Animals; Chromatography, High Pressure Liquid; Female; Follicle Stimulating Hormone; Glutamic Acid; Gonadotropin-Releasing Hormone; Hypothalamus; Luteinizing Hormone; Male; Phenols; Pregnancy; Prenatal Exposure Delayed Effects; Radioimmunoassay; Rats; Rats, Wistar; Sexual Maturation; Testosterone
Año:2010
Volumen:31
Número:4
Página de inicio:512
Página de fin:516
Título revista:Neuroendocrinology Letters
Título revista abreviado:Neuroendocrinol. Lett.
ISSN:0172780X
CODEN:NLETD
CAS:4,4' isopropylidenediphenol, 80-05-7; follitropin, 9002-68-0; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; gonadorelin, 33515-09-2, 9034-40-6; luteinizing hormone, 39341-83-8, 9002-67-9; testosterone, 58-22-0; Follicle Stimulating Hormone, 9002-68-0; Glutamic Acid, 56-86-0; Gonadotropin-Releasing Hormone, 33515-09-2; Luteinizing Hormone, 9002-67-9; Phenols; Testosterone, 58-22-0; bisphenol A, 80-05-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0172780X_v31_n4_p512_Cardoso

Referencias:

  • Akingbemi, B.T., Sottas, C.M., Koulova, A.I., Klinefelter, G.R., Hardy, M.P., Inhibition of Testicular Steroidogenesis by the Xenoestrogen Bisphenol a Is Associated with Reduced Pituitary Luteinizing Hormone Secretion and Decreased Steroidogenic Enzyme Gene Expression in Rat Leydig Cells (2004) Endocrinology, 145 (2), pp. 592-603. , DOI 10.1210/en.2003-1174
  • Brann, D.W., Mahesh, V.B., Excitatory amino acid regulation of gonadotrophin secretion: Modulation by steroid hormone (1992) J. Steroid Biochem. Mol. Biol., 41, pp. 847-850
  • Brotons, J.A., Olea Serrano, M.F., Villalobos, M., Olea, N., Xenoestrogens released from laquer coating in food cans (1995) Environ Health Perspect, 103, pp. 608-612
  • Cagen, S.Z., Waechter Jr., J.M., Dimond, S.S., Breslin, W.J., Butala, J.H., Jekat, F.W., Normal reproductive organ development in Wistar rats exposed to bisphenol A in the drinking water (1999) Regul. Toxicol. Pharmacol., 30, pp. 130-139
  • Colborn, T., Vom Saal, F.S., Soto, A.M., Developmental effects of endocrine disrupting chemicals in wildlife and humans (1993) Environ Health Perspect, 101, pp. 378-384
  • Crisp, T.M., Cleeg, E.D., Cooper, R.L., Wood, W.P., Anderson, D.G., Baetche, K.P., Environmental endocrine disruption: An effects assessment and analysis (1998) Environ Health Perspect, 106 (SUPPL. 1), pp. 11-56
  • Funabashi, T., Kawaguchi, M., Furuta, M., Fukushima, A., Kimura, F., Exposure to bisphenol a during gestation and lactation causes loss of sex difference in corticotropin-releasing hormone-immunoreactive neurons in the bed nucleus of the stria terminalis of rats (2004) Psychoneuroendocrinology, 29 (4), pp. 475-485. , DOI 10.1016/S0306-4530(03)00055-6
  • Gore, A.C., Gonadotropin-releasing hormone neurons, NMDA receptors, and their regulation by steroid hormones across the reproductive life cycle (2001) Brain Res Rev, 37 (1-3), pp. 235-248
  • Herbison, A.E., Multimodal influence of estrogen upon gonadotropin-releasing hormone neurons (1998) Endocr. Rev., 19, pp. 302-330
  • Hrabovszky, E., Steinhauser, A.M., Barabás, K., Shughrue, J., Petersen, S., Merchenthaler, I., Estrogen receptor β immunoreactivity in luteinizing hormone-releasing hormone neurons of the rat brain (2000) Endocrinology, 142 (7), pp. 3261-3264
  • Jarry, H., Hirsch, B., Leonhardt, S., Wuttke, W., Amino acid neurotransmitter release in the preoptic area of rats during the positive feedback actions of estradiol on LH release (1992) Neuroendocrinology, 56, pp. 133-140
  • Krishnan, A.V., Stathis, P., Permuth, S.F., Tokes, L., Feldman, D., Bisphenol-A: An estrogenic substance is released from polycarbonate flasks during autoclaving (1993) Endocrinology, 132 (6), pp. 2279-2286. , DOI 10.1210/en.132.6.2279
  • Kubo, K., Arai, O., Omura, M., Watanabe, R., Ogata, R., Aou, S., Low dose effects of bisphenol a on sexual differentiation of the brain and behavior in rats (2003) Neuroscience Research, 45 (3), pp. 345-356. , DOI 10.1016/S0168-0102(02)00251-1
  • Matthews, J.B., Twoney, K., Zacharewski, T.R., In vitro and in vivo interactions of bisphenol A and its metabolite, bisphenol A glucuronide, with estrogen receptors α and β (2001) Chem Res Toxicol, 14, pp. 149-157
  • Moguilevsky, J.A., Wutke, W., Changes in the control of gonadotrophin secretion by neurotransmitters during sexual development in rats (2001) Exp Clin Endocrinol Diabetes, 109, pp. 188-195
  • Mooradian, A.D., Morley, J.E., Biological action of androgens (1987) Endocr. Rev., 8, pp. 1-28
  • Moschos, S., Chan, J., Mantzoros, C., Leptin and reproduction: A review (2002) Fertil and Steril, 77 (3), pp. 433-444
  • Mostafa, R.M., Mirghani, Z., Moustafa, K.M., Moustafa, Y.M., El Hefnawi, M.H., New Chapter in old story: Endocrine disruptors and male reproductive system (2007) JMSR Journal of Medical Sciences Research, 2, pp. 33-40
  • Nelson, P., Epidemiology, biology and endocrine disruptors (2003) Occup Environ Med, 60, pp. 541-542
  • Olea, N., Pulgar, R., Perez, P., Olea-Serrano, F., Rivas, A., Novillo-Fertrell, A., Pedraza, V., Sonnenschein, C., Estrogenicity of resin-based composites and sealants used in dentistry (1996) Environmental Health Perspectives, 104 (3), pp. 298-305
  • Pflieger, B.S., Schuppe, H.C., Schill, W.B., The male reproductive system and its susceptibility to endocrine disruptors chemicals (2004) Andrologia, 36, pp. 337-345
  • Prins, G.S., Neonatal estrogen exposure induces lobe-specific alterations in adult rat prostate androgen receptor expression (1992) Endocrinology, 130, pp. 3703-3714
  • Rettori, V., Kamat, A., McCann, S.M., Nitric oxide mediates the stimulation of luteinizing-hormone releasing hormone release induced by glutamic acid in vitro (1994) Brain Research Bulletin, 33 (5), pp. 501-503. , DOI 10.1016/0361-9230(94)90074-4
  • Roy, D., Angelini, N.L., Belsham, D.D., Estrogen directly represses gonadotropin-releasing hormone (GnRH) gene expression in estrogen receptor-alpha (ER alpha)- And ERbeta-expressing GT1-7 GnRH neurons (1999) Endocrinology, 140 (11), pp. Xx
  • Rochira, V., Zirilli, L., Genazzani, A.D., Balestrieri, A., Aranda, C., Fabre, B., Hypothalamic pituitary-gonadal axis in two men with aromatase deficiency: Evidence that circulating estrogens are required at the hypothalamic level for the integrity of gonadotropin negative feedback (2006) Eur. J. Endocrinol., 155, pp. 513-522
  • Rubin, B.S., Murray, M.K., Damassa, D.A., King, J.C., Soto, A.M., Perinatal exposure to low doses of bisphenol a affects body weight, patterns of estrous cyclicity, and plasma LH levels (2001) Environmental Health Perspectives, 109 (7), pp. 675-680
  • Sharpe, R.M., The roles of oestrogen in the male (1998) Trends Endocrinol Metab, 9, pp. 371-377
  • Thind, K.K., Goldsmith, P.C., Expression of estrogen and progesterone receptors in glutamate and GABA neurons of the prepubertal female monkey hypothalamus (1997) Neuroendocrinology, 65 (5), pp. 314-324
  • Tohei, A., Suda, S., Taya, K., Hashimoto, T., Kogo, H., Bisphenol A inhibits testicular functions and increases luteinizing hormone secretion in adult male rats (2001) Exp Biol Med, 226 (3), pp. 216-221
  • Toppari, J., Larsen, J.C., Christiansen, P., Giwercman, A., Grandjean, P., Guillette Jr., L.J., Male reproductive health and environmental xenoestrogens (1996) Environ Health Perspect, 104 (SUPPL. 4), pp. 741-803
  • Vom Saal, F.S., Cooke, P.S., Buchanan, D.L., Palanza, P., Thayer, K.A., Nagel, S.C., A physiologically based approach to the study of Bisphenol A and other estrogenic chemicals on the size of reproductive organs, daily sperm production and behavior (1998) Toxicol Ind Health, 14, pp. 239-260
  • Watanabe, S., Wang, R.S., Miyagawa, M., Kobayashi, K., Suda, M., Imbalance of testosterone level in male offspring of rats perinatally exposed to bisphenol A (2003) Ind Health, 41 (4), pp. 338-341

Citas:

---------- APA ----------
Cardoso, N., Pandolfi, M., Ponzo, O., Carbone, S., Szwarcfarb, B., Scacchi, P. & Reynoso, R. (2010) . Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats. Neuroendocrinology Letters, 31(4), 512-516.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0172780X_v31_n4_p512_Cardoso [ ]
---------- CHICAGO ----------
Cardoso, N., Pandolfi, M., Ponzo, O., Carbone, S., Szwarcfarb, B., Scacchi, P., et al. "Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats" . Neuroendocrinology Letters 31, no. 4 (2010) : 512-516.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0172780X_v31_n4_p512_Cardoso [ ]
---------- MLA ----------
Cardoso, N., Pandolfi, M., Ponzo, O., Carbone, S., Szwarcfarb, B., Scacchi, P., et al. "Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats" . Neuroendocrinology Letters, vol. 31, no. 4, 2010, pp. 512-516.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0172780X_v31_n4_p512_Cardoso [ ]
---------- VANCOUVER ----------
Cardoso, N., Pandolfi, M., Ponzo, O., Carbone, S., Szwarcfarb, B., Scacchi, P., et al. Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats. Neuroendocrinol. Lett. 2010;31(4):512-516.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0172780X_v31_n4_p512_Cardoso [ ]