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Our aim in this work was to further characterize the complexity and specificity of the three different isoforms (Tpk1, Tpk2 and Tpk3) of the catalytic and regulatory (Bcy1) subunits of PKA in Saccharomyces cerevisiae. We thus analyzed the subcellular localization of the PKA subunits in living cells by using strains carrying GFP (green fluorescent protein) fused to each PKA subunit. During exponential growth on glucose, both Bcy1 and Tpk2 localized in the nucleus, whereas Tpk1 and Tpk3 showed a mixed pattern of nucleo-cytoplasmic localization. During exponential growth on glycerol and during stationary phase, the PKA subunits showed mostly cytoplasmic localization, with the peculiarity that Tpk2 and Tpk3 but not Bcy1, were found associated to P-bodies and EGP bodies. Tpk3 was accumulated into P-bodies during glucose deprivation and hyper osmotic stress. Deletion of Tpk3 altered the kinetics of P-body formation. Analysis of protein expression showed that the relative expression pattern of each Tpk changes from low levels under fermentative metabolism to higher levels during stationary phase. The increase in Tpk levels produced an imbalance with Bcy1 levels. Our data suggest that the signaling specificity through PKA in yeast could be mediated by a particular subcellular localization of each isoform of Tpk. © 2009 Elsevier GmbH. All rights reserved.


Documento: Artículo
Título:Differential localization to cytoplasm, nucleus or P-bodies of yeast PKA subunits under different growth conditions
Autor:Tudisca, V.; Recouvreux, V.; Moreno, S.; Boy-Marcotte, E.; Jacquet, M.; Portela, P.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Buenos Aires 1428, Argentina
Institut de Génétique et Microbiologie, Université Paris Sud 11, CNRS UMR8621, Bâtiment 400, F-91405 Orsay Cedex, France
Palabras clave:P-bodies; PKA subunits; Saccharomyces cerevisiae; Stationary phase; cyclic AMP dependent protein kinase; fungal protein; glucose; glycerol; green fluorescent protein; protein Bcy1; protein Tpk1; protein Tpk2; protein Tpk3; unclassified drug; culture medium; cyclic AMP dependent protein kinase; green fluorescent protein; isoenzyme; protein subunit; article; carbon source; cell nucleus; cellular distribution; controlled study; cytoplasm; fermentation; fungal strain; fungus growth; gene deletion; hyperosmotic stress; kinetics; nonhuman; nuclear localization signal; priority journal; protein analysis; protein expression; Saccharomyces cerevisiae; cell granule; chemistry; culture medium; cytology; enzymology; genetics; growth, development and aging; metabolism; protein subunit; protein transport; reproducibility; Cell Nucleus; Culture Media; Cyclic AMP-Dependent Protein Kinases; Cytoplasm; Cytoplasmic Granules; Green Fluorescent Proteins; Isoenzymes; Protein Subunits; Protein Transport; Reproducibility of Results; Saccharomyces cerevisiae; Saccharomyces cerevisiae; Cell Nucleus; Culture Media; Cyclic AMP-Dependent Protein Kinases; Cytoplasm; Cytoplasmic Granules; Green Fluorescent Proteins; Isoenzymes; Protein Subunits; Protein Transport; Reproducibility of Results; Saccharomyces cerevisiae
Página de inicio:339
Página de fin:348
Título revista:European Journal of Cell Biology
Título revista abreviado:Eur. J. Cell Biol.
CAS:glucose, 50-99-7, 84778-64-3; glycerol, 56-81-5; cyclic AMP dependent protein kinase; Culture Media; Cyclic AMP-Dependent Protein Kinases,; Green Fluorescent Proteins, 147336-22-9; Isoenzymes; Protein Subunits; Culture Media; Cyclic AMP-Dependent Protein Kinases; Green Fluorescent Proteins; Isoenzymes; Protein Subunits


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---------- APA ----------
Tudisca, V., Recouvreux, V., Moreno, S., Boy-Marcotte, E., Jacquet, M. & Portela, P. (2010) . Differential localization to cytoplasm, nucleus or P-bodies of yeast PKA subunits under different growth conditions. European Journal of Cell Biology, 89(4), 339-348.
---------- CHICAGO ----------
Tudisca, V., Recouvreux, V., Moreno, S., Boy-Marcotte, E., Jacquet, M., Portela, P. "Differential localization to cytoplasm, nucleus or P-bodies of yeast PKA subunits under different growth conditions" . European Journal of Cell Biology 89, no. 4 (2010) : 339-348.
---------- MLA ----------
Tudisca, V., Recouvreux, V., Moreno, S., Boy-Marcotte, E., Jacquet, M., Portela, P. "Differential localization to cytoplasm, nucleus or P-bodies of yeast PKA subunits under different growth conditions" . European Journal of Cell Biology, vol. 89, no. 4, 2010, pp. 339-348.
---------- VANCOUVER ----------
Tudisca, V., Recouvreux, V., Moreno, S., Boy-Marcotte, E., Jacquet, M., Portela, P. Differential localization to cytoplasm, nucleus or P-bodies of yeast PKA subunits under different growth conditions. Eur. J. Cell Biol. 2010;89(4):339-348.