Abstract:
While changes in heme oxygenase (HO-1) in lung cancer have already been reported, conflicting results were obtained for enzyme expression in human lung cancer specimens. Therefore, the aim of this work was to study HO-1 expression in a large collection of human lung cancer samples. For this purpose, we analyzed the expression of HO-1 in an organized tissue microarray (TMA) and investigated its correlation with clinicopathological data. Ninety-six percent of tumor samples were positive for HO-1, and the expression of HO-1 was significantly higher in cancerous than in non-cancerous tissues. Importantly, HO-1 expression correlated with advanced stages and lymph node involvement. Additionally, quantitative RT-PCR in 18 pairs of human lung carcinomas and their adjacent non-malignant tissues was performed. Our results demonstrate that HO-1 protein is upregulated in epithelial malignant cells in NSCLC and its expression is associated with higher stages of the disease. Additionally, different subcellular localization is observed between tumor and adjacent non-malignant tissues. © 2012 Elsevier Ireland Ltd.
Registro:
Documento: |
Artículo
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Título: | Expression of heme oxygenase-1 in non-small cell lung cancer (NSCLC) and its correlation with clinical data |
Autor: | Degese, M.S.; Mendizabal, J.E.; Gandini, N.A.; Gutkind, J.S.; Molinolo, A.; Hewitt, S.M.; Curino, A.C.; Coso, O.A.; Facchinetti, M.M. |
Filiación: | Laboratorio de Fisiología y Biología Molecular, IFIBYNE - CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina Laboratorio de Biología del Cáncer, INIBIBB, CCT-Bahía Blanca - CONICET, 8000, Bahía Blanca, Argentina Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD, United States Tarp/AMPL NCI Advanced Technology Center, Bethesda, MD, United States
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Palabras clave: | Heme-oxygenase-1; Immunohistochemistry; Lung cancer; NSCLC; Tissue microarray; heme oxygenase 1; adult; article; cancer staging; female; histopathology; human; human cell; human tissue; immunohistochemistry; lung carcinoma; lung non small cell cancer; lymph node; major clinical study; male; priority journal; protein expression; protein function; real time polymerase chain reaction; tissue microarray; upregulation; Animals; Carcinoma, Non-Small-Cell Lung; Cell Line, Tumor; Epithelial Cells; Female; Gene Expression; Heme Oxygenase-1; Humans; Kaplan-Meier Estimate; Lung Neoplasms; Lymphatic Metastasis; Male; Mice; NIH 3T3 Cells; Real-Time Polymerase Chain Reaction; Tissue Array Analysis; Up-Regulation |
Año: | 2012
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Volumen: | 77
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Número: | 1
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Página de inicio: | 168
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Página de fin: | 175
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DOI: |
http://dx.doi.org/10.1016/j.lungcan.2012.02.016 |
Título revista: | Lung Cancer
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Título revista abreviado: | Lung Cancer
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ISSN: | 01695002
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CODEN: | LUCAE
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CAS: | HMOX1 protein, human, 1.14.99.3; Heme Oxygenase-1, 1.14.99.3
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01695002_v77_n1_p168_Degese |
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Citas:
---------- APA ----------
Degese, M.S., Mendizabal, J.E., Gandini, N.A., Gutkind, J.S., Molinolo, A., Hewitt, S.M., Curino, A.C.,..., Facchinetti, M.M.
(2012)
. Expression of heme oxygenase-1 in non-small cell lung cancer (NSCLC) and its correlation with clinical data. Lung Cancer, 77(1), 168-175.
http://dx.doi.org/10.1016/j.lungcan.2012.02.016---------- CHICAGO ----------
Degese, M.S., Mendizabal, J.E., Gandini, N.A., Gutkind, J.S., Molinolo, A., Hewitt, S.M., et al.
"Expression of heme oxygenase-1 in non-small cell lung cancer (NSCLC) and its correlation with clinical data"
. Lung Cancer 77, no. 1
(2012) : 168-175.
http://dx.doi.org/10.1016/j.lungcan.2012.02.016---------- MLA ----------
Degese, M.S., Mendizabal, J.E., Gandini, N.A., Gutkind, J.S., Molinolo, A., Hewitt, S.M., et al.
"Expression of heme oxygenase-1 in non-small cell lung cancer (NSCLC) and its correlation with clinical data"
. Lung Cancer, vol. 77, no. 1, 2012, pp. 168-175.
http://dx.doi.org/10.1016/j.lungcan.2012.02.016---------- VANCOUVER ----------
Degese, M.S., Mendizabal, J.E., Gandini, N.A., Gutkind, J.S., Molinolo, A., Hewitt, S.M., et al. Expression of heme oxygenase-1 in non-small cell lung cancer (NSCLC) and its correlation with clinical data. Lung Cancer. 2012;77(1):168-175.
http://dx.doi.org/10.1016/j.lungcan.2012.02.016