Artículo
Castro, G.R.; Stettler, A.O.; Ferrero, M.A.; Siñeriz, F. "Selection of an extracellular esterase-producing microorganism" (1992) Journal of Industrial Microbiology. 10(3-4):165-168
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Abstract:
The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of a selected strain characterized as belonging to the Bacillus subtilis group showed a high level of extracellular activity at pH 6.6 to 8.0 and 35°C. In crude extracts the optimum enzymatic activity was obtained at 35°C and pH 7.0. © 1992 Society for Industrial Microbiology.
Registro:
| Documento: | Artículo |
| Título: | Selection of an extracellular esterase-producing microorganism |
| Autor: | Castro, G.R.; Stettler, A.O.; Ferrero, M.A.; Siñeriz, F. |
| Filiación: | Depto. de Qca. Biológica, Fac. de Ciencias Exactas y Naturales, Univ. de Buenos Aires. Pab. II-Cdad. Universitaria (Nuñez), Buenos Aires, Argentina Cátedra de Microbiología Superior, Facultad de Quimica, Bioquímica y Farmacia, Univ. Nac. de Tucumán-PROIMI-MIRCEN, Avda. Belgrano y Pje, Caseros, Tucumán, 4000, Argentina |
| Palabras clave: | B. subtilis esterase; Esterase production; Esterase screening test; pH profile; Temperature profile; Batch cell culture; Biotechnology; Enzymes; Microbiology; Esterase plate screening; Esterase producing microorganisms; Extracellular esterases; Microorganisms; esterase; article; bacillus subtilis; microorganism; nonhuman; ph; priority journal; screening; temperature |
| Año: | 1992 |
| Volumen: | 10 |
| Número: | 3-4 |
| Página de inicio: | 165 |
| Página de fin: | 168 |
| DOI: | http://dx.doi.org/10.1007/BF01569761 |
| Título revista: | Journal of Industrial Microbiology |
| Título revista abreviado: | J. Ind. Microbiol. |
| ISSN: | 01694146 |
| CODEN: | JIMIE |
| CAS: | esterase, 9013-79-0 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01694146_v10_n3-4_p165_Castro |
Referencias:
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- Schär, H.P., Gyrax, D., Ramos Tombo, G.M., Ghisalba, D., Purification of an acetyl specific carboxylesterase from Nocardia mediterranei (1988) Appl. Microbiol. Biotechnol., 27, pp. 451-456
- Sneath, P.H.A., Endospore forming Gram positive rods and cocci (1986) Bergey's Manual of Systematic Bacteriology, pp. 1104-1139. , P.H.A., Sneath, 9th Edn., The William and Wilkins Co., Baltimore
Citas:
---------- APA ----------
Castro, G.R., Stettler, A.O., Ferrero, M.A. & Siñeriz, F. (1992) . Selection of an extracellular esterase-producing microorganism. Journal of Industrial Microbiology, 10(3-4), 165-168.http://dx.doi.org/10.1007/BF01569761
---------- CHICAGO ----------
Castro, G.R., Stettler, A.O., Ferrero, M.A., Siñeriz, F. "Selection of an extracellular esterase-producing microorganism" . Journal of Industrial Microbiology 10, no. 3-4 (1992) : 165-168.http://dx.doi.org/10.1007/BF01569761
---------- MLA ----------
Castro, G.R., Stettler, A.O., Ferrero, M.A., Siñeriz, F. "Selection of an extracellular esterase-producing microorganism" . Journal of Industrial Microbiology, vol. 10, no. 3-4, 1992, pp. 165-168.http://dx.doi.org/10.1007/BF01569761
---------- VANCOUVER ----------
Castro, G.R., Stettler, A.O., Ferrero, M.A., Siñeriz, F. Selection of an extracellular esterase-producing microorganism. J. Ind. Microbiol. 1992;10(3-4):165-168.http://dx.doi.org/10.1007/BF01569761
