Abstract:
The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of a selected strain characterized as belonging to the Bacillus subtilis group showed a high level of extracellular activity at pH 6.6 to 8.0 and 35°C. In crude extracts the optimum enzymatic activity was obtained at 35°C and pH 7.0. © 1992 Society for Industrial Microbiology.
Registro:
Documento: |
Artículo
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Título: | Selection of an extracellular esterase-producing microorganism |
Autor: | Castro, G.R.; Stettler, A.O.; Ferrero, M.A.; Siñeriz, F. |
Filiación: | Depto. de Qca. Biológica, Fac. de Ciencias Exactas y Naturales, Univ. de Buenos Aires. Pab. II-Cdad. Universitaria (Nuñez), Buenos Aires, Argentina Cátedra de Microbiología Superior, Facultad de Quimica, Bioquímica y Farmacia, Univ. Nac. de Tucumán-PROIMI-MIRCEN, Avda. Belgrano y Pje, Caseros, Tucumán, 4000, Argentina
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Palabras clave: | B. subtilis esterase; Esterase production; Esterase screening test; pH profile; Temperature profile; Batch cell culture; Biotechnology; Enzymes; Microbiology; Esterase plate screening; Esterase producing microorganisms; Extracellular esterases; Microorganisms; esterase; article; bacillus subtilis; microorganism; nonhuman; ph; priority journal; screening; temperature |
Año: | 1992
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Volumen: | 10
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Número: | 3-4
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Página de inicio: | 165
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Página de fin: | 168
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DOI: |
http://dx.doi.org/10.1007/BF01569761 |
Título revista: | Journal of Industrial Microbiology
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Título revista abreviado: | J. Ind. Microbiol.
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ISSN: | 01694146
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CODEN: | JIMIE
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CAS: | esterase, 9013-79-0
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01694146_v10_n3-4_p165_Castro |
Referencias:
- Debavov, V.G., The industrial use of Bacilli (1982) The Molecular Biology of the Bacilli, pp. 331-370. , D., Dubnau, Academic Press, New York
- Gordon, R.E., The genus Bacillus (1977) Handbook of Microbiology, pp. 319-336. , A.I., Laskin, H., Lechevalier, CRC Press, Boca Raton, FL
- Iwai, M., Okomura, S., Tsujisaka, Y., Synthesis of terpene alcohol esters by lipace (1980) Agricultural and Biological Chemistry, 44, pp. 2731-2732
- Mamas, S., Millet, J., Purification et propriétés estérase excrétée pendant la sporulation de Bacillus subtilis (1975) Biochimie, 57, pp. 9-16
- Owusu, R.K., Cowan, D.A., Isolation and partial characterization of a novel thermostable carboxylesterase from a thermophilic Bacillus (1991) Enzyme Microb. Technol., 13, pp. 158-163
- Poutamen, K., Sundberg, M., An acetyl esterase of Trichoderma reesei and its role in the hydrolysis of acetyl xylans (1988) Applied Microbiology and Biotechnology, 28, pp. 419-424
- Priest, F.G., Goodfellow, C.T., Todd, C.A., A numerical classification of the genus Bacillus (1988) J. Gen. Microbiol., 134, pp. 1847-1882
- Schär, H.P., Gyrax, D., Ramos Tombo, G.M., Ghisalba, D., Purification of an acetyl specific carboxylesterase from Nocardia mediterranei (1988) Appl. Microbiol. Biotechnol., 27, pp. 451-456
- Sneath, P.H.A., Endospore forming Gram positive rods and cocci (1986) Bergey's Manual of Systematic Bacteriology, pp. 1104-1139. , P.H.A., Sneath, 9th Edn., The William and Wilkins Co., Baltimore
Citas:
---------- APA ----------
Castro, G.R., Stettler, A.O., Ferrero, M.A. & Siñeriz, F.
(1992)
. Selection of an extracellular esterase-producing microorganism. Journal of Industrial Microbiology, 10(3-4), 165-168.
http://dx.doi.org/10.1007/BF01569761---------- CHICAGO ----------
Castro, G.R., Stettler, A.O., Ferrero, M.A., Siñeriz, F.
"Selection of an extracellular esterase-producing microorganism"
. Journal of Industrial Microbiology 10, no. 3-4
(1992) : 165-168.
http://dx.doi.org/10.1007/BF01569761---------- MLA ----------
Castro, G.R., Stettler, A.O., Ferrero, M.A., Siñeriz, F.
"Selection of an extracellular esterase-producing microorganism"
. Journal of Industrial Microbiology, vol. 10, no. 3-4, 1992, pp. 165-168.
http://dx.doi.org/10.1007/BF01569761---------- VANCOUVER ----------
Castro, G.R., Stettler, A.O., Ferrero, M.A., Siñeriz, F. Selection of an extracellular esterase-producing microorganism. J. Ind. Microbiol. 1992;10(3-4):165-168.
http://dx.doi.org/10.1007/BF01569761