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Abstract:

We evaluated the in vitro phosphorylation of the presynaptic substrate of protein kinase C (PKC), GAP-43/B-50 and the PKC activity in the striatum of rats submitted to a circling training (CT) test during postnatal development. Motor activity at 30 days of age, but not at other ages, produced a unilateral reduction (-29.5%; p < 0.001) in the level of GAP- 43/B-50 endogenous phosphorylation in the contralateral striatum with respect to the ipsilateral side, while non-trained control animals did not show asymmetric differences. Compared to controls, the contralateral striatum of trained animals also showed a significant reduction (-29.3%; p < 0.001) in the incorporation of 32P-phosphate into GAP-43. This decreased in vitro GAP-43 phosphorylation was seen at 30 min, but not immediately after circling motor behavior. This contralateral change in GAP-43 phosphorylation correlated with the running speed developed by the animals [(r = 0.9443, p = 0.0046, n = 6, relative to control group) and (r = 0.8813, p = 0.0203, n = 6, with respect to the ipsilateral side of the exercised animals)]. On the contrary, GAP-43/B-50 immunoblots did not show changes in the amount of this phosphoprotein among the different experiment groups. Back phosphorylation assays, performed in the presence of bovine purified PKC, increased the level of GAP-43/B-50 phosphorylation in the striatum contralateral to the sense of turning [(+22%; p < 0.05, with respect to ipsilateral side of the same trained group) and (+21%; p < 0.05, relative to control group)]. Taken together, these results demonstrate that the activity developed in the CT test induces a reduction in the phosphorylation state of GAP-43/B-50 in the specific site for PKC. We conclude that general markers of activity-dependent neuronal plasticity are also altered in the same period that long-lasting changes in striatal neuroreceptors are triggered by circling motor behavior.

Registro:

Documento: Artículo
Título:Decreased GAP-43/B-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development
Autor:Paratcha, G.Ch.; Ibarra, G.R.; Wolansky, M.J.; Rodriguez, J.A.; Azcurra, J.M.
Filiación:Laboratorio de Biologia Celular, Universidad de Buenos Aires (UBA), Ciudad Universitaria, Pabellon II, 1428 Buenos Aires, Argentina
C.F. Melo 4127, 1602 Buenos Aires, Argentina
Palabras clave:Circling training; Development; GAP-43/B-50; PKC; Rat striatum; SPM; neuromodulin; protein kinase c; animal experiment; animal tissue; article; circling behavior; controlled study; corpus striatum; enzyme activity; enzyme substrate; male; motor performance; nerve cell plasticity; nonhuman; priority journal; protein phosphorylation; rat; synaptic membrane; Animals; Behavior, Animal; Blotting, Western; Cell Membrane; Conditioning (Psychology); Corpus Striatum; GAP-43 Protein; Locomotion; Male; Motor Neurons; Phosphorylation; Protein Kinase C; Rats; Rats, Sprague-Dawley; Synapses
Año:1999
Volumen:65
Número:1
Página de inicio:34
Página de fin:43
DOI: http://dx.doi.org/10.1016/S0169-328X(98)00327-1
Título revista:Molecular Brain Research
Título revista abreviado:Mol. Brain Res.
ISSN:0169328X
CODEN:MBREE
CAS:GAP-43 Protein; Protein Kinase C, EC 2.7.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0169328X_v65_n1_p34_Paratcha

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Citas:

---------- APA ----------
Paratcha, G.Ch., Ibarra, G.R., Wolansky, M.J., Rodriguez, J.A. & Azcurra, J.M. (1999) . Decreased GAP-43/B-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development. Molecular Brain Research, 65(1), 34-43.
http://dx.doi.org/10.1016/S0169-328X(98)00327-1
---------- CHICAGO ----------
Paratcha, G.Ch., Ibarra, G.R., Wolansky, M.J., Rodriguez, J.A., Azcurra, J.M. "Decreased GAP-43/B-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development" . Molecular Brain Research 65, no. 1 (1999) : 34-43.
http://dx.doi.org/10.1016/S0169-328X(98)00327-1
---------- MLA ----------
Paratcha, G.Ch., Ibarra, G.R., Wolansky, M.J., Rodriguez, J.A., Azcurra, J.M. "Decreased GAP-43/B-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development" . Molecular Brain Research, vol. 65, no. 1, 1999, pp. 34-43.
http://dx.doi.org/10.1016/S0169-328X(98)00327-1
---------- VANCOUVER ----------
Paratcha, G.Ch., Ibarra, G.R., Wolansky, M.J., Rodriguez, J.A., Azcurra, J.M. Decreased GAP-43/B-50 phosphorylation in striatal synaptic plasma membranes after circling motor behavior during development. Mol. Brain Res. 1999;65(1):34-43.
http://dx.doi.org/10.1016/S0169-328X(98)00327-1