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Abstract:

UDP-Glc:protein transglucosylase (UPTG) (EC 2.4.1.112), involved in the first step of a two-step mechanism proposed in potato tuber for protein-bound α-glucan synthesis, was found in developing maize endosperm. The enzyme transfers glucosyl residues from UDP-[14C]glucose to an endogenous protein acceptor in a Mn2+-dependent reaction in the absence of added primer. After SDS-polycrylamide gel electrophrresis of the reaction product, radioactivity co-migrated with a 38-kDa protein band. A two-step experiment shows that the developing endosperm of maize contains phosphorylase and starch synthase, capable of utilizing the UPTG reaction product formed during the first step as a primer for the synthesis of protein-bound glucan in the presence of the respective glucosyl donors during the second incubation step. © 1994.

Registro:

Documento: Artículo
Título:UDP-glucose:protein transglucosylase in developing maize endosperm
Autor:Rothschild, A.; Tandecarz, J.S.
Filiación:Instituto de Investigaciones Bioquimicas Fundacion Campomar and Facultad de Ciencias Exactas y Naturales, Universidad de Buenas Aires, Instituto de Investigaciones Bioquimicas Buenos Aires, Consejo Nacional de Investigaciones Cientificas y Técnicas, Av. Patricias Argentinas 435, (1405) Buenos Aires, Argentina
Palabras clave:Developing maize endosperm; Protein acceptor; protein transglucosylase; UDP-Glc; Zea mays; Zea mays
Año:1994
Volumen:97
Número:2
Página de inicio:119
Página de fin:127
DOI: http://dx.doi.org/10.1016/0168-9452(94)90048-5
Título revista:Plant Science
Título revista abreviado:Plant Sci.
ISSN:01689452
CODEN:PLSCE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v97_n2_p119_Rothschild

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Citas:

---------- APA ----------
Rothschild, A. & Tandecarz, J.S. (1994) . UDP-glucose:protein transglucosylase in developing maize endosperm. Plant Science, 97(2), 119-127.
http://dx.doi.org/10.1016/0168-9452(94)90048-5
---------- CHICAGO ----------
Rothschild, A., Tandecarz, J.S. "UDP-glucose:protein transglucosylase in developing maize endosperm" . Plant Science 97, no. 2 (1994) : 119-127.
http://dx.doi.org/10.1016/0168-9452(94)90048-5
---------- MLA ----------
Rothschild, A., Tandecarz, J.S. "UDP-glucose:protein transglucosylase in developing maize endosperm" . Plant Science, vol. 97, no. 2, 1994, pp. 119-127.
http://dx.doi.org/10.1016/0168-9452(94)90048-5
---------- VANCOUVER ----------
Rothschild, A., Tandecarz, J.S. UDP-glucose:protein transglucosylase in developing maize endosperm. Plant Sci. 1994;97(2):119-127.
http://dx.doi.org/10.1016/0168-9452(94)90048-5