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Abstract:

The effect of alkaline pH on the stability of chloroplast fructose-1,6-bisphosphatase was examined. The incubation of the native enzyme (tetramer) at pH 8.9 lowered the specific activity (t0.5 = 6 min). The presence of fructose 1,6-bisphosphatase and Ca2+, two enzyme modulators, delayed the inactivation process (t0.5 = 50 min) whereas sodium trichloroacetate, a chaotropic anion, accelerated it (t0.5 < 1 min). On the other hand, the conversion of 60% of the enzyme to dimers required an incubation of 3 h at pH 8.9. On this basis, it appeared that an inactive tetramer form preceded the dissociation of chloroplast fructose-1,6-bisphosphatase at pH 8.9; both fructose 1,6-bisphosphate and Ca2+ prevented enzyme inactivation by stabilizing the tetramer form. These results indicated that, in addition to their roles as enzyme modulators, sugar biphosphates and bivalent cations contribute to stabilizing the active conformation of chloroplast fructose-1,6-bisphosphatase. © 1990.

Registro:

Documento: Artículo
Título:Effect of alkaline pH on the activity and the structure of chloroplast fructose-1,6-bisphosphatase
Autor:Ballicora, M.A.; Wolosiuk, R.A.
Filiación:Instituto de Investigaciones Bioquimicas (Fundacion Campomar, IIBBA-CONICET, and FCEN-UBA), Buenos Aires, Argentina
Palabras clave:chloroplasts; enzyme dissociation; fructose-1,6-bisphosphatase; high pH
Año:1990
Volumen:70
Número:1
Página de inicio:35
Página de fin:41
DOI: http://dx.doi.org/10.1016/0168-9452(90)90029-N
Título revista:Plant Science
Título revista abreviado:Plant Sci.
ISSN:01689452
CODEN:PLSCE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v70_n1_p35_Ballicora

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Citas:

---------- APA ----------
Ballicora, M.A. & Wolosiuk, R.A. (1990) . Effect of alkaline pH on the activity and the structure of chloroplast fructose-1,6-bisphosphatase. Plant Science, 70(1), 35-41.
http://dx.doi.org/10.1016/0168-9452(90)90029-N
---------- CHICAGO ----------
Ballicora, M.A., Wolosiuk, R.A. "Effect of alkaline pH on the activity and the structure of chloroplast fructose-1,6-bisphosphatase" . Plant Science 70, no. 1 (1990) : 35-41.
http://dx.doi.org/10.1016/0168-9452(90)90029-N
---------- MLA ----------
Ballicora, M.A., Wolosiuk, R.A. "Effect of alkaline pH on the activity and the structure of chloroplast fructose-1,6-bisphosphatase" . Plant Science, vol. 70, no. 1, 1990, pp. 35-41.
http://dx.doi.org/10.1016/0168-9452(90)90029-N
---------- VANCOUVER ----------
Ballicora, M.A., Wolosiuk, R.A. Effect of alkaline pH on the activity and the structure of chloroplast fructose-1,6-bisphosphatase. Plant Sci. 1990;70(1):35-41.
http://dx.doi.org/10.1016/0168-9452(90)90029-N