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Abstract:

Screening of indigenous bacteria for the presence of genetic information for the biosynthesis of storage polymers was performed in river water samples using PCR amplification combined with Southern blotting. Polyhydroxyalkanoate synthase genes and polyphosphate kinase genes were detected using template DNA from different laboratory and river isolated bacterial strains, and from total river water DNA. This approach could be a suitable alternative to the study of bacterial communities based on the analysis of genes instead of microorganisms.

Registro:

Documento: Artículo
Título:Detection of reserve polymer synthesis genes in natural bacterial populations
Autor:López, N.I.; Pettinari, M.J.; Méndez, B.S.
Filiación:Depto. de Ciencias Biológicas, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Depto. de Quim. Biológica, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, 1428-Buenos Aires, Argentina
Palabras clave:bacterial survival; natural water; pha genes; polyhydroxyalkanoate; polyphosphate; ppk genes; polymer; polyphosphate; river water; acinetobacter; article; bacillus; bacterium isolation; biosynthesis; controlled study; ecology; escherichia coli; genetic analysis; nonhuman; polymerase chain reaction; priority journal
Año:1997
Volumen:22
Número:2
Página de inicio:129
Página de fin:136
DOI: http://dx.doi.org/10.1016/S0168-6496(96)00083-9
Título revista:FEMS Microbiology Ecology
Título revista abreviado:FEMS MICROBIOL. ECOL.
ISSN:01686496
CODEN:FMECE
CAS:polyphosphate, 13813-62-2, 8017-16-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01686496_v22_n2_p129_Lopez

Referencias:

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Citas:

---------- APA ----------
López, N.I., Pettinari, M.J. & Méndez, B.S. (1997) . Detection of reserve polymer synthesis genes in natural bacterial populations. FEMS Microbiology Ecology, 22(2), 129-136.
http://dx.doi.org/10.1016/S0168-6496(96)00083-9
---------- CHICAGO ----------
López, N.I., Pettinari, M.J., Méndez, B.S. "Detection of reserve polymer synthesis genes in natural bacterial populations" . FEMS Microbiology Ecology 22, no. 2 (1997) : 129-136.
http://dx.doi.org/10.1016/S0168-6496(96)00083-9
---------- MLA ----------
López, N.I., Pettinari, M.J., Méndez, B.S. "Detection of reserve polymer synthesis genes in natural bacterial populations" . FEMS Microbiology Ecology, vol. 22, no. 2, 1997, pp. 129-136.
http://dx.doi.org/10.1016/S0168-6496(96)00083-9
---------- VANCOUVER ----------
López, N.I., Pettinari, M.J., Méndez, B.S. Detection of reserve polymer synthesis genes in natural bacterial populations. FEMS MICROBIOL. ECOL. 1997;22(2):129-136.
http://dx.doi.org/10.1016/S0168-6496(96)00083-9