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Abstract:

The hepatitis B virus X protein (HBV-X) is a multifunctional regulatory protein associated with the pathogenesis of liver disease in chronic HBV infection. Basal core promoter mutations (BCP), associated with the clinical course of chronic HBV infection, affect HBV-X at 130–131 positions. The role of these mutations on HBV-X biological activity remains largely unknown. The aim of this study was to analyze the impact of the presence of different amino acids at 130–131 positions of HBV-X on the biological activity of the protein. Transient expression of wild type and mutant F1b and F4 HBV-X increased cell mortality by the induction of apoptosis in human hepatoma cells. The wild type and mutant HBV-X differentially modulate the expression of pro-apoptotic (Bax) and anti-apoptotic (Bcl-2 and Bcl-X) regulatory proteins of the Bcl-2 family. Furthermore, the expression of HBV-X variants of both subgenotypes induced autophagy of human tumoral hepatocytes. In conclusion, HBV-X variants of the Latin American HBV F genotype promotes human hepatocytes death by the induction of apoptosis and autophagy. The results of this work describe some of the molecular mechanisms by which HBV-X variants contribute to the pathogenesis of liver diseases in the infected liver and help to the biological characterization of genotype F, responsible of the majority of HBV infections in Argentina. © 2017

Registro:

Documento: Artículo
Título:X protein variants of the autochthonous Latin American hepatitis B virus F genotype promotes human hepatocyte death by the induction of apoptosis and autophagy
Autor:Elizalde, M.M.; Campos, R.H.; Barbini, L.
Filiación:Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Microbiología, Inmunología y Biotecnología, Cátedra de Virología, Buenos Aires, Argentina
Universidad Nacional de Mar del Plata, Facultad de Ciencias Exactas y Naturales, Departamento de Química, Mar del Plata, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Palabras clave:Apoptosis; Autophagy; Basal core promoter mutation; Genotype F; Hepatitis B virus; Hepatitis B virus X protein; cell DNA; hepatitis B virus X protein; protein Bax; protein bcl 2; protein bcl x; apoptosis; Article; autophagosome; autophagy; cell membrane; cell nucleus membrane; cell structure; cell vacuole; chromatin condensation; controlled study; fluorescence microscopy; hepatitis B; Hepatitis B virus; Hepatitis B virus genotype F; hepatoma cell; human; human cell; liver cell; nonhuman; priority journal; signal transduction; transient expression; transient transfection; wild type
Año:2017
Volumen:242
Página de inicio:156
Página de fin:165
DOI: http://dx.doi.org/10.1016/j.virusres.2017.09.025
Título revista:Virus Research
Título revista abreviado:Virus Res.
ISSN:01681702
CODEN:VIRED
CAS:protein bcl 2, 219306-68-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v242_n_p156_Elizalde

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Citas:

---------- APA ----------
Elizalde, M.M., Campos, R.H. & Barbini, L. (2017) . X protein variants of the autochthonous Latin American hepatitis B virus F genotype promotes human hepatocyte death by the induction of apoptosis and autophagy. Virus Research, 242, 156-165.
http://dx.doi.org/10.1016/j.virusres.2017.09.025
---------- CHICAGO ----------
Elizalde, M.M., Campos, R.H., Barbini, L. "X protein variants of the autochthonous Latin American hepatitis B virus F genotype promotes human hepatocyte death by the induction of apoptosis and autophagy" . Virus Research 242 (2017) : 156-165.
http://dx.doi.org/10.1016/j.virusres.2017.09.025
---------- MLA ----------
Elizalde, M.M., Campos, R.H., Barbini, L. "X protein variants of the autochthonous Latin American hepatitis B virus F genotype promotes human hepatocyte death by the induction of apoptosis and autophagy" . Virus Research, vol. 242, 2017, pp. 156-165.
http://dx.doi.org/10.1016/j.virusres.2017.09.025
---------- VANCOUVER ----------
Elizalde, M.M., Campos, R.H., Barbini, L. X protein variants of the autochthonous Latin American hepatitis B virus F genotype promotes human hepatocyte death by the induction of apoptosis and autophagy. Virus Res. 2017;242:156-165.
http://dx.doi.org/10.1016/j.virusres.2017.09.025