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Abstract:

Closely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. Three isolates of BoHV-5, previously classified as subtype “BoHV-5b”, were phylogenetically studied and analyzed via eight PCR sequencing assays dispersed at regular intervals throughout the genome to discriminate between BoHV-1 and BoHV-5. In the phylogenetic analysis, differences of clustering were found in the UL27 gene which encodes the glycoprotein B (gB). We detected two recombination breakpoints in the open reading frame of the UL27 gene. We compared the amino acid sequences of the gB of BoHV-1.1 and 1.2, BoHV-5a and recombinant formerly named BoHV-5b (chimeric gB) and subsequently performed molecular modeling. All structures were alike and, simultaneously, similar to the chimeric gB. Neutralizing antibodies against BoHV-1, BoHV-5 and recombinant viruses were analyzed via serum virus neutralization test using polyclonal sera and a monoclonal antibody against gB to demonstrate an absence of viral escape for both assays. Our results show that homologous recombination between two related species of ruminant alphaherpesviruses can occur in natural field conditions. We found three recombinant field isolates, previously classified as BoHV-5b subtypes, between BoHV-1 and BoHV-5. © 2017 Elsevier B.V.

Registro:

Documento: Artículo
Título:Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
Autor:Maidana, S.S.; Craig, P.O.; Craig, M.I.; Ludwig, L.; Mauroy, A.; Thiry, E.; Romera, S.A.
Filiación:Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto de tecnología Agropecuaria (INTA), N. Repetto y Los Reseros S/N, CC25, Castelar, Buenos Aires B1712WAA, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Rivadavia 1917, Ciudad Autónoma de Buenos Aires, C1033AAJ, Argentina
Cátedra de Inmunogenética, Facultad de Ciencias exactas, Químicas y Naturales, Universidad de Morón, Cabildo 134, Morón, Buenos Aires B1708JPD, Argentina
Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN-UBA/CONICET) y Departamento de Química Biológica, FCEN, UBA, Argentina
Veterinary Virology and Animal Viral Diseases, Fundamental and Applied Research on Animal Health center and Faculty of Veterinary Medicine, University of Liège, Boulevard de Colonster, 20, B43b, Liège, B-4000, Belgium
Cátedra de Inmunología, Universidad del Salvador, Champagnat 1599, Ruta Panamericana, Km 54.5 PilarProvincia de Buenos Aires B1630AHU, Argentina
Palabras clave:Alphaherpesviruses; Bovine herpesvirus 1 (BoHV-1); Bovine herpesvirus 5 (BoHV-5); Glycoprotein B; Natural recombination; neutralizing antibody; virus DNA; Alphaherpesvirinae; amino acid sequence; animal cell; Article; bovine alphaherpesvirus 1; bovine alphaherpesvirus 5; comparative study; DNA sequence; genetic code; genetic recombination; homologous recombination; MDBK cell line; molecular model; nonhuman; open reading frame; phylogenetic tree; phylogeny; priority journal; virus isolation; virus neutralization; virus recombinant; animal; bovine; Bovine herpesvirus 1; Bovine herpesvirus 5; chemistry; classification; cluster analysis; genetic variation; genetics; genotype; homologous recombination; isolation and purification; polymerase chain reaction; Animals; Cattle; Cluster Analysis; DNA, Viral; Genetic Variation; Genotype; Herpesvirus 1, Bovine; Herpesvirus 5, Bovine; Homologous Recombination; Phylogeny; Polymerase Chain Reaction; Sequence Analysis, DNA
Año:2017
Volumen:242
Página de inicio:122
Página de fin:130
DOI: http://dx.doi.org/10.1016/j.virusres.2017.09.018
Título revista:Virus Research
Título revista abreviado:Virus Res.
ISSN:01681702
CODEN:VIRED
CAS:DNA, Viral
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v242_n_p122_Maidana

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Citas:

---------- APA ----------
Maidana, S.S., Craig, P.O., Craig, M.I., Ludwig, L., Mauroy, A., Thiry, E. & Romera, S.A. (2017) . Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5. Virus Research, 242, 122-130.
http://dx.doi.org/10.1016/j.virusres.2017.09.018
---------- CHICAGO ----------
Maidana, S.S., Craig, P.O., Craig, M.I., Ludwig, L., Mauroy, A., Thiry, E., et al. "Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5" . Virus Research 242 (2017) : 122-130.
http://dx.doi.org/10.1016/j.virusres.2017.09.018
---------- MLA ----------
Maidana, S.S., Craig, P.O., Craig, M.I., Ludwig, L., Mauroy, A., Thiry, E., et al. "Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5" . Virus Research, vol. 242, 2017, pp. 122-130.
http://dx.doi.org/10.1016/j.virusres.2017.09.018
---------- VANCOUVER ----------
Maidana, S.S., Craig, P.O., Craig, M.I., Ludwig, L., Mauroy, A., Thiry, E., et al. Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5. Virus Res. 2017;242:122-130.
http://dx.doi.org/10.1016/j.virusres.2017.09.018