Abstract:
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are cellular factors involved in the replication of several viruses. In this study we analyzed the expression and intracellular localization of hnRNP A2 and hnRNP K in cell cultures infected with two viruses that cause human hemorrhagic fevers: dengue virus type 2 (DENV-2) and Junín virus (JUNV). We determined that DENV-2 promoted the cytoplasmic translocation of hnRNP K and to a lesser extent of hnRNP A2, meanwhile, JUNV infection induced an increase in hnRNP K cytoplasmic localization whereas hnRNP A2 remained mainly in the nucleus of infected cells. Both hnRNP K and hnRNP A2 were localized predominantly in the nucleus of JUNV persistently-infected cells even after superinfection with JUNV indicating that persistent infection does not alter nucleo-cytoplasmic transport of these hnRNPs. Total levels of hnRNP K expression were unaffected by DENV-2 or JUNV infection. In addition we determined, using small interfering RNAs, that hnRNP K knockout inhibits DENV-2 and JUNV multiplication. Our results indicate that DENV-2 and JUNV induce hnRNP K cytoplasmic translocation to favor viral multiplication. © 2015 Elsevier B.V.
Registro:
Documento: |
Artículo
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Título: | The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication |
Autor: | Brunetti, J.E.; Scolaro, L.A.; Castilla, V. |
Filiación: | Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Ciudad Autónoma de Buenos Aires, Piso 4, C1428EGA, Argentina
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Palabras clave: | Dengue virus; Heterogeneous nuclear ribonucleoproteins; HnRNP A2; HnRNP K; Junín virus; heterogeneous nuclear ribonucleoprotein; heterogeneous nuclear ribonucleoprotein A2; heterogeneous nuclear ribonucleoprotein K; host factor; unclassified drug; virus protein; heterogeneous nuclear ribonucleoprotein K; hnRNP A2; A549 cell line; animal cell; Article; cell culture; controlled study; cytoplasm; Dengue virus 2; genetic transfection; human; human cell; immunofluorescence test; Junin virus; nonhuman; persistent infection; plasmid; priority journal; protein expression; protein localization; superinfection; supernatant; Vero cell line; virogenesis; Western blotting; animal; cell line; cell nucleus; chemistry; Dengue virus; host pathogen interaction; Junin virus; metabolism; physiology; virus replication; Dengue virus; Dengue virus type 2; Animals; Cell Line; Cell Nucleus; Cytoplasm; Dengue Virus; Heterogeneous-Nuclear Ribonucleoprotein Group A-B; Heterogeneous-Nuclear Ribonucleoprotein K; Host-Pathogen Interactions; Humans; Junin virus; Virus Replication |
Año: | 2015
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Volumen: | 203
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Página de inicio: | 84
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Página de fin: | 91
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DOI: |
http://dx.doi.org/10.1016/j.virusres.2015.04.001 |
Título revista: | Virus Research
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Título revista abreviado: | Virus Res.
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ISSN: | 01681702
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CODEN: | VIRED
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CAS: | Heterogeneous-Nuclear Ribonucleoprotein Group A-B; Heterogeneous-Nuclear Ribonucleoprotein K; hnRNP A2
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v203_n_p84_Brunetti |
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Citas:
---------- APA ----------
Brunetti, J.E., Scolaro, L.A. & Castilla, V.
(2015)
. The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication. Virus Research, 203, 84-91.
http://dx.doi.org/10.1016/j.virusres.2015.04.001---------- CHICAGO ----------
Brunetti, J.E., Scolaro, L.A., Castilla, V.
"The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication"
. Virus Research 203
(2015) : 84-91.
http://dx.doi.org/10.1016/j.virusres.2015.04.001---------- MLA ----------
Brunetti, J.E., Scolaro, L.A., Castilla, V.
"The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication"
. Virus Research, vol. 203, 2015, pp. 84-91.
http://dx.doi.org/10.1016/j.virusres.2015.04.001---------- VANCOUVER ----------
Brunetti, J.E., Scolaro, L.A., Castilla, V. The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication. Virus Res. 2015;203:84-91.
http://dx.doi.org/10.1016/j.virusres.2015.04.001