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Abstract:

The aim of the present study was to analyze the influence of virus origin, mammalian or mosquito cell-derived, on antiviral susceptibility of DENV-2 to entry inhibitors and the association of this effect with any alteration in the mode of entry into the cell. To this end, ten serial passages of DENV-2 were performed in mosquito C6/36 cells or monkey Vero cells and the antiviral susceptibility of each virus passage to sulfated polysaccharides (SPs), like heparin and carrageenans, was evaluated by a virus plaque reduction assay. After serial passaging in Vero cells, DENV-2 became increasingly resistant to SP inhibition whereas the antiviral susceptibility was not altered in virus propagated in C6/36 cells. The change in antiviral susceptibility was associated to a differential mode of entry into the host cell. The route of endocytic entry for productive Vero cell infection was altered from a non-classical clathrin independent pathway for C6/36-grown virus to a clathrin-mediated endocytosis when the virus was serially propagated in Vero cells. Our results show the impact of the cellular system used for successive propagation of DENV on the initial interaction between the host cell and the virion in the next round of infection and the relevant consequences it might have during the in vitro evaluation of entry inhibitors. © 2014 Elsevier B.V.

Registro:

Documento: Artículo
Título:Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells
Autor:Acosta, E.G.; Piccini, L.E.; Talarico, L.B.; Castilla, V.; Damonte, E.B.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
IQUIBICEN-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Universitaria, Pabellón 2, Piso 4, 1428 Buenos Aires, Argentina
Department of Infectious Diseases, Molecular Virology, Heidel-berg University, 69120 Heidelberg, Germany
Fundación Infant, 1406 Buenos Aires, Argentina
Palabras clave:Antiviral activity; Carrageenan; Clathrin-mediated endocytosis; Dengue virus; Entry inhibitor; Sulfated polysaccharide; carrageenan; chlorpromazine; heparin; antivirus agent; carrageenan; heparin; virus RNA; antiviral activity; antiviral susceptibility; article; controlled study; Dengue virus 1; Dengue virus 2; endocytosis; host cell; in vitro study; nonhuman; priority journal; Vero cell; virus entry; adaptation; animal; antiviral resistance; cell line; Chlorocebus aethiops; Dengue virus; DNA sequence; drug effects; genetics; growth, development and aging; molecular genetics; mosquito; nucleotide sequence; viral plaque assay; virus culture; Adaptation, Biological; Animals; Antiviral Agents; Carrageenan; Cell Line; Cercopithecus aethiops; Culicidae; Dengue Virus; DNA Mutational Analysis; Drug Resistance, Viral; Endocytosis; Heparin; Molecular Sequence Data; RNA, Viral; Sequence Analysis, DNA; Serial Passage; Viral Plaque Assay; Virus Internalization
Año:2014
Volumen:184
Página de inicio:39
Página de fin:43
DOI: http://dx.doi.org/10.1016/j.virusres.2014.02.011
Título revista:Virus Research
Título revista abreviado:Virus Res.
ISSN:01681702
CODEN:VIRED
CAS:carrageenan, 9000-07-1, 9049-05-2, 9061-82-9, 9064-57-7; chlorpromazine, 50-53-3, 69-09-0; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; Antiviral Agents; Carrageenan; Heparin; RNA, Viral
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v184_n_p39_Acosta

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Citas:

---------- APA ----------
Acosta, E.G., Piccini, L.E., Talarico, L.B., Castilla, V. & Damonte, E.B. (2014) . Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells. Virus Research, 184, 39-43.
http://dx.doi.org/10.1016/j.virusres.2014.02.011
---------- CHICAGO ----------
Acosta, E.G., Piccini, L.E., Talarico, L.B., Castilla, V., Damonte, E.B. "Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells" . Virus Research 184 (2014) : 39-43.
http://dx.doi.org/10.1016/j.virusres.2014.02.011
---------- MLA ----------
Acosta, E.G., Piccini, L.E., Talarico, L.B., Castilla, V., Damonte, E.B. "Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells" . Virus Research, vol. 184, 2014, pp. 39-43.
http://dx.doi.org/10.1016/j.virusres.2014.02.011
---------- VANCOUVER ----------
Acosta, E.G., Piccini, L.E., Talarico, L.B., Castilla, V., Damonte, E.B. Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells. Virus Res. 2014;184:39-43.
http://dx.doi.org/10.1016/j.virusres.2014.02.011