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Abstract:

The entry of dengue virus-1 (DENV-1) strain Hawaii into mosquito C6/36 cells was analyzed using a variety of biochemical inhibitors together with electron microscopy. The treatment with ammonium chloride, chlorpromazine, dansylcadaverine and dynasore inhibited virus yields, determined by infectivity titrations, whereas nystatin and methyl-β-cyclodextrin did not have any effect. The effect of the clathrin and dynamin inhibitors on DENV-1 entry was corroborated by detection of internalized virions using immunofluorescence staining. Furthermore, electron micrographs showed the incoming virions attached to electron-dense invaginations of the plasma membrane and within coated vesicles that resembled clathrin-coated pits and vesicles, respectively. The susceptibility to clathrin and dynamin inhibitors of clinical isolates from recent outbreaks was comparable to that shown by the cell culture-adapted reference strain. Similarly, DENV-3 strain H87 and DENV-4 strain 8124 were also inhibited in the presence of ammonium chloride, chlorpromazine and dynasore, allowing conclude that the infectious entry of DENV serotypes to mosquito cells occurs by low pH-dependent clathrin-mediated endocytosis. © 2011 Elsevier B.V.

Registro:

Documento: Artículo
Título:Infectious dengue-1 virus entry into mosquito C6/36 cells
Autor:Acosta, E.G.; Castilla, V.; Damonte, E.B.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Piso 4, 1428 Buenos Aires, Argentina
Palabras clave:Clathrin-mediated endocytosis; Dengue virus-1; Mosquito cells; Virus entry; ammonium chloride; chlorpromazine; clathrin; dansylcadaverine; dynamin; enzyme inhibitor; methyl beta cyclodextrin; reagent; animal cell; article; cell membrane; coated vesicle; controlled study; dengue; Dengue virus 1; Dengue virus 3; Dengue virus 4; electron microscopy; endocytosis; immunofluorescence; insect cell; insect cell culture; mosquito; nonhuman; pH; priority journal; virion; virus entry; virus isolation; virus strain; virus titration; Animals; Cell Line; Clathrin-Coated Vesicles; Culicidae; Dengue Virus; Endocytosis; Microscopy, Electron; Virus Internalization; Dengue virus
Año:2011
Volumen:160
Número:1-2
Página de inicio:173
Página de fin:179
DOI: http://dx.doi.org/10.1016/j.virusres.2011.06.008
Título revista:Virus Research
Título revista abreviado:Virus Res.
ISSN:01681702
CODEN:VIRED
CAS:ammonium chloride, 12125-02-9; chlorpromazine, 50-53-3, 69-09-0; dansylcadaverine, 10121-91-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v160_n1-2_p173_Acosta

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Citas:

---------- APA ----------
Acosta, E.G., Castilla, V. & Damonte, E.B. (2011) . Infectious dengue-1 virus entry into mosquito C6/36 cells. Virus Research, 160(1-2), 173-179.
http://dx.doi.org/10.1016/j.virusres.2011.06.008
---------- CHICAGO ----------
Acosta, E.G., Castilla, V., Damonte, E.B. "Infectious dengue-1 virus entry into mosquito C6/36 cells" . Virus Research 160, no. 1-2 (2011) : 173-179.
http://dx.doi.org/10.1016/j.virusres.2011.06.008
---------- MLA ----------
Acosta, E.G., Castilla, V., Damonte, E.B. "Infectious dengue-1 virus entry into mosquito C6/36 cells" . Virus Research, vol. 160, no. 1-2, 2011, pp. 173-179.
http://dx.doi.org/10.1016/j.virusres.2011.06.008
---------- VANCOUVER ----------
Acosta, E.G., Castilla, V., Damonte, E.B. Infectious dengue-1 virus entry into mosquito C6/36 cells. Virus Res. 2011;160(1-2):173-179.
http://dx.doi.org/10.1016/j.virusres.2011.06.008