Abstract:
Mal de Río Cuarto virus (MRCV) is a plant virus of the genus Fijivirus within the family Reoviridae that infects several monocotyledonous species and is transmitted by planthoppers in a persistent and propagative manner. Other members of the family replicate in viral inclusion bodies (VIBs) termed viroplasms that are formed in the cytoplasm of infected plant and insect cells. In this study, the protein coded by the first ORF of MRCV segment S9 (P9-1) was shown to establish cytoplasmic inclusion bodies resembling viroplasms after transfection of Spodoptera frugiperda insect cells. In accordance, MRCV P9-1 self-associates giving rise to high molecular weight complexes when expressed in bacteria. Strong self-interaction was also evidenced by yeast two-hybrid assays. Furthermore, biochemical characterization showed that MRCV P9-1 bound single stranded RNA and had ATPase activity. Finally, the MRCV P9-1 region required for the formation of VIB-like structures was mapped to the protein carboxy-terminal half. This extensive functional and biochemical characterization of MRCV P9-1 revealed further similarities between plant and animal reovirus viroplasm proteins. © 2010 Elsevier B.V.
Registro:
Documento: |
Artículo
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Título: | Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts |
Autor: | Maroniche, G.A.; Mongelli, V.C.; Peralta, A.V.; Distéfano, A.J.; Llauger, G.; Taboga, O.A.; Hopp, E.H.; del Vas, M. |
Filiación: | Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (IB-INTA), Las Cabañas y Los Reseros, s/n. Hurlingham CP 1686, Buenos Aires, Argentina Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA Buenos Aires, Argentina
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Palabras clave: | ATPase; Fijivirus; Inclusion body; MRCV; Plant reovirus; Self-interaction; SsRNA binding; Viroplasm; adenosine triphosphatase; single stranded RNA; virus protein; article; bacterium; controlled study; enzyme activity; Lepidoptera; Mal de Rio Cuarto virus; molecular weight; nonhuman; nucleotide sequence; open reading frame; priority journal; Reovirus; virus inclusion; yeast; animal; chemistry; genetics; metabolism; protein motif; Reoviridae; Spodoptera; virology; Amino Acid Motifs; Animals; Inclusion Bodies, Viral; Open Reading Frames; Reoviridae; Spodoptera; Viral Proteins; Animalia; Archaeorrhyncha; Fijivirus; Hexapoda; Mal de Rio Cuarto virus; Orthoreovirus; Reoviridae; Spodoptera frugiperda; Amino Acid Motifs; Animals; Inclusion Bodies, Viral; Open Reading Frames; Reoviridae; Spodoptera; Viral Proteins |
Año: | 2010
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Volumen: | 152
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Número: | 1-2
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Página de inicio: | 96
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Página de fin: | 103
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DOI: |
http://dx.doi.org/10.1016/j.virusres.2010.06.010 |
Título revista: | Virus Research
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Título revista abreviado: | Virus Res.
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ISSN: | 01681702
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CODEN: | VIRED
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CAS: | adenosine triphosphatase, 37289-25-1, 9000-83-3; Viral Proteins; Viral Proteins
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v152_n1-2_p96_Maroniche |
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Citas:
---------- APA ----------
Maroniche, G.A., Mongelli, V.C., Peralta, A.V., Distéfano, A.J., Llauger, G., Taboga, O.A., Hopp, E.H.,..., del Vas, M.
(2010)
. Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts. Virus Research, 152(1-2), 96-103.
http://dx.doi.org/10.1016/j.virusres.2010.06.010---------- CHICAGO ----------
Maroniche, G.A., Mongelli, V.C., Peralta, A.V., Distéfano, A.J., Llauger, G., Taboga, O.A., et al.
"Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts"
. Virus Research 152, no. 1-2
(2010) : 96-103.
http://dx.doi.org/10.1016/j.virusres.2010.06.010---------- MLA ----------
Maroniche, G.A., Mongelli, V.C., Peralta, A.V., Distéfano, A.J., Llauger, G., Taboga, O.A., et al.
"Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts"
. Virus Research, vol. 152, no. 1-2, 2010, pp. 96-103.
http://dx.doi.org/10.1016/j.virusres.2010.06.010---------- VANCOUVER ----------
Maroniche, G.A., Mongelli, V.C., Peralta, A.V., Distéfano, A.J., Llauger, G., Taboga, O.A., et al. Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts. Virus Res. 2010;152(1-2):96-103.
http://dx.doi.org/10.1016/j.virusres.2010.06.010