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Abstract:

The retroviral Gag protein is the only viral product that is necessary for the assembly of virions in mammalian cells. We have established an in vitro assembly system to study the assembly properties of purified feline immunodeficiency virus (FIV) Gag protein expressed in bacteria. Under fully defined conditions, the FIV Gag protein assembles into spherical particles of 33. nm in diameter which are morphologically similar to authentic immature particles, albeit smaller than virions. The in vitro assembly of FIV Gag into particles was found to be resistant to the addition of Triton X-100 and required the presence of RNA. Notably, we found that an amino acid substitution in the nucleocapsid domain of Gag that impairs RNA binding and blocks virion production in vivo, also abrogates Gag assembly in vitro. The development of an in vitro assembly system for FIV Gag protein will facilitate the study of the mechanisms by which this protein assembles into immature particles. © 2010 Elsevier B.V.

Registro:

Documento: Artículo
Título:In vitro assembly of the feline immunodeficiency virus Gag polyprotein
Autor:Affranchino, J.L.; González, S.A.
Filiación:Laboratorio de Virología, CONICET - Facultad de Ciencias Exactas y Naturales, Universidad de Belgrano (UB), Villanueva 1324, C1426BMJ Buenos Aires, Argentina
Palabras clave:Feline immunodeficiency virus; Gag polyprotein; In vitro assembly; Lentiviruses; Gag polyprotein; RNA; triton x 100; unclassified drug; virus protein; amino acid substitution; article; controlled study; Feline immunodeficiency virus; in vitro study; nonhuman; particle size; priority journal; protein assembly; protein domain; protein expression; RNA binding; virion; virus inhibition; virus nucleocapsid; virus particle; Amino Acid Substitution; Detergents; Escherichia coli; Gene Products, gag; Immunodeficiency Virus, Feline; Mutagenesis, Site-Directed; Octoxynol; Recombinant Proteins; RNA, Viral; RNA-Binding Proteins; Virion; Virus Assembly; Feline immunodeficiency virus; Mammalia
Año:2010
Volumen:150
Número:1-2
Página de inicio:153
Página de fin:157
DOI: http://dx.doi.org/10.1016/j.virusres.2010.03.012
Título revista:Virus Research
Título revista abreviado:Virus Res.
ISSN:01681702
CODEN:VIRED
CAS:RNA, 63231-63-0; Detergents; Gene Products, gag; Octoxynol, 9002-93-1; RNA, Viral; RNA-Binding Proteins; Recombinant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v150_n1-2_p153_Affranchino

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Citas:

---------- APA ----------
Affranchino, J.L. & González, S.A. (2010) . In vitro assembly of the feline immunodeficiency virus Gag polyprotein. Virus Research, 150(1-2), 153-157.
http://dx.doi.org/10.1016/j.virusres.2010.03.012
---------- CHICAGO ----------
Affranchino, J.L., González, S.A. "In vitro assembly of the feline immunodeficiency virus Gag polyprotein" . Virus Research 150, no. 1-2 (2010) : 153-157.
http://dx.doi.org/10.1016/j.virusres.2010.03.012
---------- MLA ----------
Affranchino, J.L., González, S.A. "In vitro assembly of the feline immunodeficiency virus Gag polyprotein" . Virus Research, vol. 150, no. 1-2, 2010, pp. 153-157.
http://dx.doi.org/10.1016/j.virusres.2010.03.012
---------- VANCOUVER ----------
Affranchino, J.L., González, S.A. In vitro assembly of the feline immunodeficiency virus Gag polyprotein. Virus Res. 2010;150(1-2):153-157.
http://dx.doi.org/10.1016/j.virusres.2010.03.012