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Abstract:

Our previous studies reported the inhibitory action against arenaviruses of antiretroviral zinc finger-reactive compounds provided by the National Cancer Institute (USA). These compounds were able to inactivate virions as well as to reduce virus yields from infected cells. Here, the inactivation of the arenavirus Junín (JUNV), agent of Argentine hemorrhagic fever, by the aromatic disulfide NSC20625 was analyzed. The treatment of purified JUNV with this compound eliminated infectivity apparently through irreversible modifications in the matrix Z protein detected by: (a) alterations in the electrophoretic migration profile of Z under non-reducing conditions; (b) an electrodense labeling in the internal layer beneath the envelope and around the matrix Z protein, in negatively stained preparations; (c) changes in the subcellular localization of Z in cells transfected with a recombinant fusion protein JUNVZ-eGFP. The infection of Vero cells with JUNV inactivated particles was blocked at the uncoating of viral nucleocapsid from endosomes, providing new evidence for a functional role of Z in this stage of arenavirus cycle. Furthermore, the inactivated JUNV particles retained the immunoreactivity of the surface glycoprotein GP1 suggesting that this disulfide may be useful in the pursuit of an inactivating agent to obtain a vaccine antigen or diagnostic tool. © 2009 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Characterization of Junín virus particles inactivated by a zinc finger-reactive compound
Autor:García, C.C.; Ellenberg, P.C.; Artuso, M.C.; Scolaro, L.A.; Damonte, E.B.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellon 2, Piso 4, 1428 Buenos Aires, Argentina
Palabras clave:Arenavirus; Junín virus; Matrix protein; Virus inactivation; Zinc finger; disulfide; disulfide nsc20625; enhanced green fluorescent protein; fatty acid binding protein; hybrid protein; matrix protein; unclassified drug; animal cell; article; cellular distribution; controlled study; electrophoresis; endosome; genetic transfection; hemorrhagic fever; Junin virus; nonhuman; priority journal; virogenesis; virus inactivation; virus infectivity; virus nucleocapsid; virus particle; zinc finger motif; Animals; Anti-HIV Agents; Arenaviridae Infections; Azo Compounds; Carrier Proteins; Cercopithecus aethiops; Disulfides; Green Fluorescent Proteins; Junin virus; Microscopy, Electron, Transmission; Nucleocapsid Proteins; Recombinant Fusion Proteins; Vero Cells; Virion; Virus Inactivation; Zinc Fingers; Arenavirus; Junin virus
Año:2009
Volumen:143
Número:1
Página de inicio:106
Página de fin:113
DOI: http://dx.doi.org/10.1016/j.virusres.2009.03.010
Título revista:Virus Research
Título revista abreviado:Virus Res.
ISSN:01681702
CODEN:VIRED
CAS:disulfide, 16734-12-6; 1,1-azobisformamide, 123-77-3; Anti-HIV Agents; Azo Compounds; Carrier Proteins; Disulfides; Green Fluorescent Proteins, 147336-22-9; Nucleocapsid Proteins; Recombinant Fusion Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v143_n1_p106_Garcia

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Citas:

---------- APA ----------
García, C.C., Ellenberg, P.C., Artuso, M.C., Scolaro, L.A. & Damonte, E.B. (2009) . Characterization of Junín virus particles inactivated by a zinc finger-reactive compound. Virus Research, 143(1), 106-113.
http://dx.doi.org/10.1016/j.virusres.2009.03.010
---------- CHICAGO ----------
García, C.C., Ellenberg, P.C., Artuso, M.C., Scolaro, L.A., Damonte, E.B. "Characterization of Junín virus particles inactivated by a zinc finger-reactive compound" . Virus Research 143, no. 1 (2009) : 106-113.
http://dx.doi.org/10.1016/j.virusres.2009.03.010
---------- MLA ----------
García, C.C., Ellenberg, P.C., Artuso, M.C., Scolaro, L.A., Damonte, E.B. "Characterization of Junín virus particles inactivated by a zinc finger-reactive compound" . Virus Research, vol. 143, no. 1, 2009, pp. 106-113.
http://dx.doi.org/10.1016/j.virusres.2009.03.010
---------- VANCOUVER ----------
García, C.C., Ellenberg, P.C., Artuso, M.C., Scolaro, L.A., Damonte, E.B. Characterization of Junín virus particles inactivated by a zinc finger-reactive compound. Virus Res. 2009;143(1):106-113.
http://dx.doi.org/10.1016/j.virusres.2009.03.010