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Abstract:

C167, a mutant derived from the XJC13 strain of Junin virus, is highly attenuated in its pathogenic properties for newborn mice. Whereas 102 PFU of XJC13 injected intracerebrally killed 100% of two-day-old mice, the mutant showed no detectable lethality. Survival of mice infected with C167 was associated with a reduced and delayed virus replication in brain and a defective spread of virus from the site of inoculation to the other tissues, including spleen, kidney, thymus, liver, peritoneal cells and serum. As an apparent consequence of the restricted replication of C167 in mice, no detectable interferon induction and low levels of neutralizing antibodies were observed. Analysis of multiplication kinetics of C167 and XJC13 in different cell cultures in vitro has confirmed that the attenuated phenotype of C167 was related to a specific inefficient replication in murine cells. This host-range restriction was due to a combination of adsorption and penetration blockage. © 1988.

Registro:

Documento: Artículo
Título:Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells
Autor:Scolaro, L.A.; Mersich, S.E.; Damonte, E.B.
Filiación:Laboratorio de Virologia, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Uniuersidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Arenavirus; Argentine hemorrhagic fever; Attenuated mutant; Junin virus; Mouse virulence; Restricted multiplication in murine cells; animal experiment; arenavirus; cell culture; junin virus; mouse; nonhuman; priority journal; virus attenuation; virus mutant; virus replication; virus virulence; Animal; Animals, Newborn; Arenaviridae; Arenaviruses, New World; Hemorrhagic Fever, American; Interferons; Mice; Mutation; Support, Non-U.S. Gov't; Virulence; Virus Replication
Año:1989
Volumen:13
Número:4
Página de inicio:283
Página de fin:294
DOI: http://dx.doi.org/10.1016/0168-1702(89)90074-9
Título revista:Virus Research
Título revista abreviado:Virus Res.
ISSN:01681702
CODEN:VIRED
CAS:Interferons, 9008-11-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v13_n4_p283_Scolaro

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Citas:

---------- APA ----------
Scolaro, L.A., Mersich, S.E. & Damonte, E.B. (1989) . Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells. Virus Research, 13(4), 283-294.
http://dx.doi.org/10.1016/0168-1702(89)90074-9
---------- CHICAGO ----------
Scolaro, L.A., Mersich, S.E., Damonte, E.B. "Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells" . Virus Research 13, no. 4 (1989) : 283-294.
http://dx.doi.org/10.1016/0168-1702(89)90074-9
---------- MLA ----------
Scolaro, L.A., Mersich, S.E., Damonte, E.B. "Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells" . Virus Research, vol. 13, no. 4, 1989, pp. 283-294.
http://dx.doi.org/10.1016/0168-1702(89)90074-9
---------- VANCOUVER ----------
Scolaro, L.A., Mersich, S.E., Damonte, E.B. Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells. Virus Res. 1989;13(4):283-294.
http://dx.doi.org/10.1016/0168-1702(89)90074-9