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Abstract:

The early events in Junín virus (JUNV) infection are not thoroughly understood. We have previously shown that JUNV enter cells by clathrin-mediated endocytosis. In this report we examine the role of microfilaments and microtubules during early virus infection. Inhibitory effects of drugs affecting main cytoskeletal components on JUNV entry into Vero cells were analyzed. Drugs that disrupted microfilaments or stabilized microtubules inhibited early steps of virus entry. In contrast, drugs that stabilized microfilaments or depolymerized microtubules were not able to block virus entry very efficiently. Furthermore, real time PCR was performed to detect viral entry and we found more than 10-fold less RNA when microfilaments were depolymerized while a 100-fold diminution was seen when microtubules were stabilized. Taken together our results demonstrate that JUNV relies on an intact actin network during early infection in Vero cells while a dynamic microtubule network is also needed. This represents an important contribution to the characterization of arenavirus multiplication cycle. © 2008 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Involvement of cytoskeleton in Junín virus entry
Autor:Martinez, M.G.; Cordo, S.M.; Candurra, N.A.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellon II, Piso 4, 1428 Buenos Aires, Argentina
Palabras clave:Actin; Cytoskeleton; Junín virus; Tubulin; cytochalasin D; jaspamide; latrunculin A; nocodazole; paclitaxel; virus RNA; animal cell; article; controlled study; Junin virus; microfilament; microtubule; nonhuman; priority journal; reverse transcription polymerase chain reaction; RNA synthesis; Vero cell; virus entry; virus infection; Animals; Arenaviridae Infections; Bicyclo Compounds, Heterocyclic; Cercopithecus aethiops; Cytoskeleton; Depsipeptides; Humans; Junin virus; Thiazolidines; Vero Cells; Virus Internalization; Arenavirus; Junin virus
Año:2008
Volumen:138
Número:1-2
Página de inicio:17
Página de fin:25
DOI: http://dx.doi.org/10.1016/j.virusres.2008.08.004
Título revista:Virus Research
Título revista abreviado:Virus Res.
ISSN:01681702
CODEN:VIRED
CAS:cytochalasin D, 22144-77-0; jaspamide, 102396-24-7; latrunculin A, 76343-93-6; nocodazole, 31430-18-9; paclitaxel, 33069-62-4; Bicyclo Compounds, Heterocyclic; Depsipeptides; jasplakinolide, 102396-24-7; latrunculin A, 76343-93-6; Thiazolidines
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v138_n1-2_p17_Martinez

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Citas:

---------- APA ----------
Martinez, M.G., Cordo, S.M. & Candurra, N.A. (2008) . Involvement of cytoskeleton in Junín virus entry. Virus Research, 138(1-2), 17-25.
http://dx.doi.org/10.1016/j.virusres.2008.08.004
---------- CHICAGO ----------
Martinez, M.G., Cordo, S.M., Candurra, N.A. "Involvement of cytoskeleton in Junín virus entry" . Virus Research 138, no. 1-2 (2008) : 17-25.
http://dx.doi.org/10.1016/j.virusres.2008.08.004
---------- MLA ----------
Martinez, M.G., Cordo, S.M., Candurra, N.A. "Involvement of cytoskeleton in Junín virus entry" . Virus Research, vol. 138, no. 1-2, 2008, pp. 17-25.
http://dx.doi.org/10.1016/j.virusres.2008.08.004
---------- VANCOUVER ----------
Martinez, M.G., Cordo, S.M., Candurra, N.A. Involvement of cytoskeleton in Junín virus entry. Virus Res. 2008;138(1-2):17-25.
http://dx.doi.org/10.1016/j.virusres.2008.08.004