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Abstract:

The movement protein (MP) TGBp1 of the potexvirus Potato virus X (PVX) is a multifunctional protein required for cell-to-cell movement within the host plant. Recent work on other plant viruses has indicated that MP phosphorylation by host kinases can regulate MP function. In this study, we demonstrate that recombinant and native TGBp1 are phosphorylated by Nicotiana tabacum extracts from both PVX-infected and non-infected leaves. The phosphorylation activity present in plant extracts has distinctive characteristics of casein kinase 2 (CK2): it is inhibited by heparin, stimulated by polylysine, and uses either ATP or GTP as phosphoryl donors. We also demonstrate that TGBp1 is efficiently phosphorylated by recombinant tobacco CK2 α subunit and by partially purified tobacco CK2. Phosphopeptide mass mapping reveals that TGBp1 is phosphorylated in Ser-165, which is localized within a CK2 consensus sequence. Our results strongly suggest that a N. tabacum kinase of the CK2 family is involved in TBGp1 phosphorylation during the course of viral infection. © 2008.

Registro:

Documento: Artículo
Título:Phosphorylation of the TGBp1 movement protein of Potato virus X by a Nicotiana tabacum CK2-like activity
Autor:Módena, N.A.; Zelada, A.M.; Conte, F.; Mentaberry, A.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, CONICET, FCEN-UBA, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:CK2; Phosphorylation; PVX; Triple gene block; Viral mobilization; casein kinase II; protein tgbp1; unclassified drug; virus protein; alpha chain; article; controlled study; in vitro study; nonhuman; nucleotide sequence; Potexvirus; priority journal; protein expression; protein function; protein phosphorylation; tobacco; viral plant disease; virus infection; Casein Kinase II; Molecular Sequence Data; Phosphorylation; Potexvirus; Recombinant Proteins; Serine; Tobacco; Viral Proteins; Virus Replication; Nicotiana tabacum; Potato virus X; Potexvirus
Año:2008
Volumen:137
Número:1
Página de inicio:16
Página de fin:23
DOI: http://dx.doi.org/10.1016/j.virusres.2008.04.007
Título revista:Virus Research
Título revista abreviado:Virus Res.
ISSN:01681702
CODEN:VIRED
CAS:Casein Kinase II, EC 2.7.1.37; Recombinant Proteins; Serine, 56-45-1; Viral Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v137_n1_p16_Modena

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Citas:

---------- APA ----------
Módena, N.A., Zelada, A.M., Conte, F. & Mentaberry, A. (2008) . Phosphorylation of the TGBp1 movement protein of Potato virus X by a Nicotiana tabacum CK2-like activity. Virus Research, 137(1), 16-23.
http://dx.doi.org/10.1016/j.virusres.2008.04.007
---------- CHICAGO ----------
Módena, N.A., Zelada, A.M., Conte, F., Mentaberry, A. "Phosphorylation of the TGBp1 movement protein of Potato virus X by a Nicotiana tabacum CK2-like activity" . Virus Research 137, no. 1 (2008) : 16-23.
http://dx.doi.org/10.1016/j.virusres.2008.04.007
---------- MLA ----------
Módena, N.A., Zelada, A.M., Conte, F., Mentaberry, A. "Phosphorylation of the TGBp1 movement protein of Potato virus X by a Nicotiana tabacum CK2-like activity" . Virus Research, vol. 137, no. 1, 2008, pp. 16-23.
http://dx.doi.org/10.1016/j.virusres.2008.04.007
---------- VANCOUVER ----------
Módena, N.A., Zelada, A.M., Conte, F., Mentaberry, A. Phosphorylation of the TGBp1 movement protein of Potato virus X by a Nicotiana tabacum CK2-like activity. Virus Res. 2008;137(1):16-23.
http://dx.doi.org/10.1016/j.virusres.2008.04.007