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Abstract:

In the present paper the in vitro antiviral activity of dehydroepiandrosterone (DHEA), epiandrosterone (EA) and 16 synthetic derivatives against Junin virus (JUNV) replication in Vero cells was studied. DHEA and EA caused a selective inhibition of the replication of JUNV and other members of the Arenaviridae family such as Pichinde virus and Tacaribe virus. The compounds were not virucidal to cell-free JUNV. The impairment of viral replication was not due to an inhibitory effect of the steroids on virus adsorption or internalization. An inhibitory effect of the compounds on JUNV protein synthesis and both intracellular and extracellular virus production was demonstrated. A partial inhibitory action on cell surface expression of JUNV glycoprotein G1 was also detected on DHEA- and EA-treated cultures. Like DHEA and EA, three compounds obtained from EA by chemical synthesis showed selectivity indexes higher than ribavirin, the only antiviral compound that has shown partial efficacy against arenavirus infections. © 2008 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junin virus replication in vitro
Autor:Acosta, E.G.; Bruttomesso, A.C.; Bisceglia, J.A.; Wachsman, M.B.; Galagovsky, L.R.; Castilla, V.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellon 2, Piso 4, C1428EGA Buenos Aires, Argentina
Departamento de Química Orgánica, UMYMFOR, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina
Palabras clave:Antiviral activity; Arenavirus; Dehydroepiandrosterone; DHEA; Junin virus; Steroid; 17 oxo 5alpha androstan 3beta yl acetate; 17 oxo 5alpha androstan 3beta,16beta yl diacetate; 17 oxo 5alpha androstan 5 en 3beta,16alpha yl diacetate; 17 oxoandrost 5 en 3beta yl acetate; 17 oxoandrost 5 en 3beta,16alpha yl diacetate; 17 oxoandrost 5 en 3beta,16beta yl diacetate; 17,17 ethylendioxy 3beta androstanol; 17,17 ethylendioxy 3beta androstanyl acetate; 17,17 ethylendioxy 3beta,6alpha androstanediol; 17,17 ethylendioxyandrost 15 en 3beta ol; 17,17 ethylendioxyandrost 5 en 3beta ol; 3beta 6alpha dihydroxy 17 androstanone; 3beta hydroxyandrost 15 en 17 one; 3beta,15alpha dihydroxy 5alpha androstan 17 one; 3beta,15beta dihydroxy 5alpha androstan 17 one; 3beta,16alpha dihydroxy 5alpha androstan 17 one; epiandrosterone; glycoprotein g1; prasterone; ribavirin; virus protein; animal cell; antiviral activity; Arenavirus; article; controlled study; cytotoxicity; drug inhibition; in vitro study; Junin virus; nonhuman; Pichinde arenavirus; priority journal; protein synthesis; Vero cell; virus particle; virus replication; virus transcription; Androsterone; Animals; Antiviral Agents; Cercopithecus aethiops; Dehydroepiandrosterone; Junin virus; Structure-Activity Relationship; Vero Cells; Viral Proteins; Virus Replication; Arenaviridae; Arenavirus; Junin virus; Pichinde virus; Tacaribe virus
Año:2008
Volumen:135
Número:2
Página de inicio:203
Página de fin:212
DOI: http://dx.doi.org/10.1016/j.virusres.2008.03.014
Título revista:Virus Research
Título revista abreviado:Virus Res.
ISSN:01681702
CODEN:VIRED
CAS:epiandrosterone, 481-29-8; prasterone, 53-43-0; ribavirin, 36791-04-5; Androsterone, 53-41-8; Antiviral Agents; Dehydroepiandrosterone, 53-43-0; Viral Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v135_n2_p203_Acosta

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Citas:

---------- APA ----------
Acosta, E.G., Bruttomesso, A.C., Bisceglia, J.A., Wachsman, M.B., Galagovsky, L.R. & Castilla, V. (2008) . Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junin virus replication in vitro. Virus Research, 135(2), 203-212.
http://dx.doi.org/10.1016/j.virusres.2008.03.014
---------- CHICAGO ----------
Acosta, E.G., Bruttomesso, A.C., Bisceglia, J.A., Wachsman, M.B., Galagovsky, L.R., Castilla, V. "Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junin virus replication in vitro" . Virus Research 135, no. 2 (2008) : 203-212.
http://dx.doi.org/10.1016/j.virusres.2008.03.014
---------- MLA ----------
Acosta, E.G., Bruttomesso, A.C., Bisceglia, J.A., Wachsman, M.B., Galagovsky, L.R., Castilla, V. "Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junin virus replication in vitro" . Virus Research, vol. 135, no. 2, 2008, pp. 203-212.
http://dx.doi.org/10.1016/j.virusres.2008.03.014
---------- VANCOUVER ----------
Acosta, E.G., Bruttomesso, A.C., Bisceglia, J.A., Wachsman, M.B., Galagovsky, L.R., Castilla, V. Dehydroepiandrosterone, epiandrosterone and synthetic derivatives inhibit Junin virus replication in vitro. Virus Res. 2008;135(2):203-212.
http://dx.doi.org/10.1016/j.virusres.2008.03.014