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Abstract:

Vesicular Stomatitis Virus (VSV) has been shown to induce apoptosis in a caspase-dependent manner, but the precise apoptotic pathway remains unknown. We found that caspases 9 and 3, but not caspase 8, were activated during VSV-induced apoptosis in infected Vero cells. Since caspase 9 is related to the mitochondrial apoptotic pathway, we analyzed some mitochondrial events such as changes in the mitochondrial transmembrane potential (Δψm) and mitochondrial release of apoptogenic proteins such as cytochrome c and the apoptosis inducing factor (AIF). We found that VSV infection triggers the dissipation of the Δψm and the release of both cytochrome c and AIF from the mitochondrial intermembrane space very early in the VSV infection. These results indicate that the trigger of apoptosis in VSV-infected cells occurs through the early activation of the mitochondrial apoptotic pathway. On the other hand, intracellular levels of the anti-apoptotic proteins, such as Bcl-2 and Bcl-xL, and the pro-apoptotic protein Bax, were assessed during viral infection. These analyses showed that as viral infection proceeded, the cellular level of Bcl-xL decreased, while the levels of Bax and Bcl-2 remained unaffected. The significance of the Bcl-xL modulation is also discussed. © 2004 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Early activation of the mitochondrial apoptotic pathway in Vesicular Stomatitis Virus-infected cells
Autor:Gadaleta, P.; Perfetti, X.; Mersich, S.; Coulombié, F.
Filiación:Department of Biological Chemistry, School of Sciences, University of Buenos Aires, Buenos Aires 1428, Argentina
Palabras clave:AIF; Apoptosis; Bcl-2 family proteins; Caspases; Cytochrome c; Mitochondrial transmembrane potential; VSV; apoptosis inducing factor; caspase 3; caspase 8; caspase 9; cytochrome c; protein Bax; protein bcl 2; protein bcl xl; animal cell; apoptosis; article; controlled study; enzyme activation; enzyme activity; host pathogen interaction; in vitro study; membrane potential; mitochondrial membrane; mitochondrion; nonhuman; priority journal; protein secretion; RNA virus; signal transduction; Vero cell; Vesicular stomatitis virus; virus infection; Vesicular stomatitis virus
Año:2005
Volumen:109
Número:1
Página de inicio:65
Página de fin:69
DOI: http://dx.doi.org/10.1016/j.virusres.2004.10.007
Título revista:Virus Research
Título revista abreviado:Virus Res.
ISSN:01681702
CODEN:VIRED
CAS:caspase 3, 169592-56-7; caspase 8; caspase 9, 180189-96-2; cytochrome c, 9007-43-6, 9064-84-0; protein bcl 2, 219306-68-0; protein bcl xl, 151033-38-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v109_n1_p65_Gadaleta

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Citas:

---------- APA ----------
Gadaleta, P., Perfetti, X., Mersich, S. & Coulombié, F. (2005) . Early activation of the mitochondrial apoptotic pathway in Vesicular Stomatitis Virus-infected cells. Virus Research, 109(1), 65-69.
http://dx.doi.org/10.1016/j.virusres.2004.10.007
---------- CHICAGO ----------
Gadaleta, P., Perfetti, X., Mersich, S., Coulombié, F. "Early activation of the mitochondrial apoptotic pathway in Vesicular Stomatitis Virus-infected cells" . Virus Research 109, no. 1 (2005) : 65-69.
http://dx.doi.org/10.1016/j.virusres.2004.10.007
---------- MLA ----------
Gadaleta, P., Perfetti, X., Mersich, S., Coulombié, F. "Early activation of the mitochondrial apoptotic pathway in Vesicular Stomatitis Virus-infected cells" . Virus Research, vol. 109, no. 1, 2005, pp. 65-69.
http://dx.doi.org/10.1016/j.virusres.2004.10.007
---------- VANCOUVER ----------
Gadaleta, P., Perfetti, X., Mersich, S., Coulombié, F. Early activation of the mitochondrial apoptotic pathway in Vesicular Stomatitis Virus-infected cells. Virus Res. 2005;109(1):65-69.
http://dx.doi.org/10.1016/j.virusres.2004.10.007