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Abstract:

An advanced encapsulation matrix that efficiently protects microalgae from harmful UV light without causing toxicity to the entrapped culture is developed based on the electrostatic adsorption of the dye Rhodamine B on silica preformed particles during sol-gel synthesis. The three microalgae (Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii) were previously immobilized in alginate following the Two-step procedure. Once entrapped in the silica gel, Rhodamine B act as an inner cut-off filter, protecting the encapsulated organisms from UV radiation. This matrix allows the sterilization of encapsulation devices without affecting the viability of the entrapped microalgae cells. The condensation of Si(IV) in the presence of silica particles with adsorbed dye generates silica matrices with good mechanical stability. Furthermore; no appreciable differences in microstructure, as assessed by SAXS (Small Angle X-ray Scattering), are caused by the addition of the dye. © 2014 Elsevier B.V.

Registro:

Documento: Artículo
Título:Rhodamine B doped silica encapsulation matrices for the protection of photosynthetic organisms
Autor:Perullini, M.; Durrieu, C.; Jobbágy, M.; Bilmes, S.A.
Filiación:INQUIMAE-DQIAQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II, C1428EHA, Buenos Aires, Argentina
Laboratoire des Sciences de l'Environnement, Ecole Nationale des Travaux Publics de l'Etat, rue Maurice Audin, 69518 Vaulx-en-Velin, Cedex, France
Palabras clave:Living-cell encapsulation; Microalgae; Sol-gel; UV-protection; Algae; Microorganisms; Silica gel; Sol-gel process; Sol-gels; Algae; Mechanical stability; Microorganisms; Silica gel; Sol-gel process; Sol-gels; X ray scattering; Chlamydomonas reinhardtii; Electrostatic adsorption; Encapsulation matrices; Micro-algae; Photosynthetic organisms; Pseudokirchneriella subcapitata; Small angle X-ray scattering; UV-protection; Living cell; UV protection; Silica; Silica; alginic acid; rhodamine B; silicon dioxide; rhodamine; rhodamine B; silicon dioxide; silica gel; adsorption; article; cell encapsulation; Chlamydomonas reinhardtii; Chlorella vulgaris; isotherm; microalga; nonhuman; priority journal; Pseudokirchneriella; radiation protection; toxicity; ultraviolet radiation; chemistry; drug effects; phase transition; photosynthesis; radiation response; ultraviolet radiation; Article; biosensor; cell viability; Chlamydomonas reinhardtii; Chlorella vulgaris; clinical assessment; clinical effectiveness; cytotoxicity; deterioration; gel filtration; harmful alga; molecular stability; radiation sterilization; Selenastrum capricornutum; synthesis; toxicity testing; X ray crystallography; Chlamydomonas reinhardtii; Chlorella vulgaris; Phase Transition; Photosynthesis; Rhodamines; Silicon Dioxide; Ultraviolet Rays; Chlamydomonas reinhardtii; Chlorella vulgaris; Selenastrum capricornutum
Año:2014
Volumen:184
Página de inicio:94
Página de fin:99
DOI: http://dx.doi.org/10.1016/j.jbiotec.2014.05.013
Título revista:Journal of Biotechnology
Título revista abreviado:J. Biotechnol.
ISSN:01681656
CODEN:JBITD
CAS:alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; rhodamine B, 81-88-9; silicon dioxide, 10279-57-9, 14464-46-1, 14808-60-7, 15468-32-3, 60676-86-0, 7631-86-9; silica gel, 63231-67-4; rhodamine B; Rhodamines; Silicon Dioxide
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v184_n_p94_Perullini

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Citas:

---------- APA ----------
Perullini, M., Durrieu, C., Jobbágy, M. & Bilmes, S.A. (2014) . Rhodamine B doped silica encapsulation matrices for the protection of photosynthetic organisms. Journal of Biotechnology, 184, 94-99.
http://dx.doi.org/10.1016/j.jbiotec.2014.05.013
---------- CHICAGO ----------
Perullini, M., Durrieu, C., Jobbágy, M., Bilmes, S.A. "Rhodamine B doped silica encapsulation matrices for the protection of photosynthetic organisms" . Journal of Biotechnology 184 (2014) : 94-99.
http://dx.doi.org/10.1016/j.jbiotec.2014.05.013
---------- MLA ----------
Perullini, M., Durrieu, C., Jobbágy, M., Bilmes, S.A. "Rhodamine B doped silica encapsulation matrices for the protection of photosynthetic organisms" . Journal of Biotechnology, vol. 184, 2014, pp. 94-99.
http://dx.doi.org/10.1016/j.jbiotec.2014.05.013
---------- VANCOUVER ----------
Perullini, M., Durrieu, C., Jobbágy, M., Bilmes, S.A. Rhodamine B doped silica encapsulation matrices for the protection of photosynthetic organisms. J. Biotechnol. 2014;184:94-99.
http://dx.doi.org/10.1016/j.jbiotec.2014.05.013