Abstract:
An advanced hybrid biosensing platform with improved optical quality is developed based on the acidic encapsulation of microalgi in silica matrices synthesized by TAFR (tetraethoxysilane derived alcohol free route). The three microalgi (Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii) were previously immobilized in alginate following the two-step procedure. Tuning the alginate protecting function with the aid of Tris-HCl buffer, the sol-gel synthesis was conducted at pH 4.0 well below the tolerance limit imposed by the encapsulated microalgae. The acidic condensation of Si(IV) generates silica matrices with outstanding optical properties that suit the requirements of biosensors based on optical detection methods. © 2014 Elsevier B.V.
Registro:
Documento: |
Artículo
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Título: | Sol-gel silica platforms for microalgae-based optical biosensors |
Autor: | Perullini, M.; Ferro, Y.; Durrieu, C.; Jobbágy, M.; Bilmes, S.A. |
Filiación: | INQUIMAE-DQIAQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. Ciudad Universitaria, Pab. II, C1428EHA Buenos Aires, Argentina Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés, UMR 5023 ENTPE, Université de Lyon, France
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Palabras clave: | Biosensor; Esterase activity; Microalgae; Optical quality; Sol-gel; Algae; Microorganisms; Silica; Sol-gel process; Sol-gels; Biosensing platforms; Chlamydomonas reinhardtii; Esterase activities; Micro-algae; Optical bio-sensors; Optical detection methods; Optical qualities; Pseudokirchneriella subcapitata; Biosensors; alginic acid; calcium alginate; silicate; silicon dioxide; tetraethoxysilane; esterase; glucuronic acid; hexuronic acid; silica gel; water pollutant; algal growth; article; biocompatibility; Chlamydomonas reinhardtii; Chlorella vulgaris; elementary particle; encapsulation; fluorescence; gelation; growth inhibition; hydrogel; nonhuman; optical biosensor; pH; polymerization; priority journal; Selenastrum capricornutum; synthesis; chemistry; enzymology; genetic procedures; isolation and purification; metabolism; microalga; procedures; small angle scattering; water pollutant; X ray diffraction; Alginates; Biosensing Techniques; Esterases; Glucuronic Acid; Hexuronic Acids; Microalgae; Scattering, Small Angle; Silica Gel; Water Pollutants, Chemical; X-Ray Diffraction |
Año: | 2014
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Volumen: | 179
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Número: | 1
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Página de inicio: | 65
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Página de fin: | 70
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DOI: |
http://dx.doi.org/10.1016/j.jbiotec.2014.02.007 |
Título revista: | Journal of Biotechnology
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Título revista abreviado: | J. Biotechnol.
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ISSN: | 01681656
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CODEN: | JBITD
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CAS: | alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; calcium alginate, 9005-35-0; silicate, 12627-13-3; silicon dioxide, 10279-57-9, 14464-46-1, 14808-60-7, 15468-32-3, 60676-86-0, 7631-86-9; esterase, 9013-79-0; glucuronic acid, 36116-79-7, 576-37-4, 6556-12-3; silica gel, 63231-67-4; Alginates; alginic acid; Esterases; Glucuronic Acid; Hexuronic Acids; Silica Gel; Water Pollutants, Chemical
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v179_n1_p65_Perullini |
Referencias:
- Détermination de l'inhibition de croissance de Scenedesmus subspicatus par une substance (1980) Norme experimentale NT90-304, , Association Française de Normalisation, Paris, France, AFNOR
- Blondeau, M., Coradin, T., Living materials from sol-gel chemistry: current challenges and perspectives (2012) J. Mater. Chem., 22, p. 22335
- Boninsegna, S., Bosetti, P., Carturan, G., Dellagiacoma, G., Dal Monte, R., Rossi, M., Encapsulation of individual pancreatic islets by sol-gel SiO 2 : a novel procedure for perspective cellular grafts (2003) J. Biotechnol., 100 (3), pp. 277-286
- Brayner, R., Couté, A., Livage, J., Perrette, C., Sicard, C., Micro-algal biosensors (2011) Anal. Bioanal. Chem., 401, pp. 581-597
- Brinker, C.J., Scherer, G.W., (1990) Sol-Gel Science: The Physics and Chemistry of Sol-Gel Processing, , Academic Press, Inc., San Diego, CA, USA
- CA Patent 2256369. A Process For Encapsulating Viable Animal Cells, 1997-12-04. Inventors: Carturan, Giovanni, Dal Monte Renzo and Muraca Mauricio. Applicant: Biosil. A.G; Chouteau, C., Dzyadevych, S., Durrieu, C., Chovelon, J.M., A bi-enzymatic whole celle biosensor for heavy metal and pesticides detection in water samples (2005) Biosens. Bioelectron., 21, pp. 273-281
- Coiffier, A., Coradin, T., Roux, C., Bouvet, O.M., Livage, J., Sol-gel encapsulation of bacteria: a comparison between alkoxide and aqueous routes (2001) J. Mater. Chem., 11, pp. 2039-2044
- Depagne, C., Roux, C., Coradin, T., How to design cell-based biosensors using the sol-gel process (2011) Anal. Bioanal.Chem., 400 (4), pp. 965-976
- Dickson, D.J., Lassetter, B., Glassy, B., Page, C.J., Yokochi, A.F.T., Ely, R.L., Diffusion of dissolved ions from wet silica sol-gel monoliths: implications for biological encapsulation (2013) Colloids Surf., B, 102, pp. 611-619
- Ferrer, M.L., Del Monte, F., Levy, D., A novel and simple alcohol-free sol-gel route for encapsulation of labile proteins (2002) Chem. Mater., 14, pp. 3619-3621
- Ferro, Y., Perullini, M., Jobbagy, M., Bilmes, S.A., Durrieu, C., Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels (2012) Sensors, 12 (12), pp. 16879-16891
- Gorman, D.S., Levine, R.P., Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi (1965) PNAS, 54, pp. 1665-1669
- Ionescu, R.E., Abu-Rabeah, K., Cosnier, S., Durrieu, C., Chovelon, J.-M., Marks, R.S., Amperometric algal Chlorella vulgaris cell biosensors based on alginate and polypyrrole-alginate gels (2006) Electroanalysis, 18 (11), pp. 1041-1046
- Kuncova, G., Podrazky, O., Ripp, S., Trögl, J., Sayler, G.S., Demnerova, K., Vankova, R., Monitoring of the viability of cells immobilized by sol-gel process (2004) J. Sol-Gel Sci. Technol., 31, pp. 1-8
- Livage, J., Coradin, T., Living cells in oxide glasses (2006) Rev. Mineral. Geochem., 64 (1), pp. 315-332
- Mandelbrot, B.B., (1983) The Fractal Geometry of Nature, , Freeman, San Francisco, CA
- Moreno-Garrido, I., Microalgae immobilization: current techniques and uses (2008) Bioresour. Technol., 99, pp. 3949-3964
- Nguyen-Ngoc, H., Durrieu, C., Tran-Minh, C., Synchronous-scan fluorescence of algal cells for toxicity assessment of heavy metals and herbicides (2009) Ecotoxicol. Environ. Saf., 72, pp. 316-320
- Hanh, N.-N., Canh, T.-M., Sol-gel process for vegetal cell encapsulation (2007) Mater. Sci. Eng., 27 (4), pp. 607-611
- Perullini, M., Jobbágy, M., Soller-Illia, G.J.A.A., Bilmes, S.A., Cellular growth at cavities created inside silica monoliths synthesized by sol-gel (2005) Chem. Mater., 17, pp. 3806-3808
- Perullini, M., Rivero, M., Jobbágy, M., Mentaberry, A., Bilmes, S.A., Plant cell proliferation inside an inorganic host (2007) J. Biotechnol., 127, pp. 542-548
- Perullini, M., Jobbágy, M., Bermúdez Moretti, M., Correa García, S., Bilmes, S.A., Optimizing silica encapsulation of living cells: in situ evaluation of cellular stress (2008) Chem. Mater., 20, pp. 3015-3021
- Perullini, M., Jobbágy, M., Bilmes, S.A., Torriani, I.L., Candal, R., Effect of synthesis conditions on the microstructure of TEOS derived silica hydrogels synthesized by the alcohol-free sol-gel route (2011) J. Sol-Gel Sci. Technol., 59, pp. 174-180
- Perullini, M., Amoura, M., Jobbágy, M., Roux, C., Livage, J., Coradin, T., Bilmes, S.A., Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach (2011) J. Mater. Chem., 21 (22), pp. 8026-8031
- Perullini, M., Jobbágy, M., Japas, M.L., Bilmes, S.A., New Method for the Simultaneous determination of Diffusion and Adsorption of dyes in Silica Hydrogels (2014) J. Colloid Interf. Sci., , doi:10.1016/j.jcis.2014.03.030
- http://kur.web.psi.ch/sans1/SANSSoft/sasfit.html; Schaefer, D.W., Keefer, K.D., Fractal geometry of silica condensation polymers (1984) Phys. Rev. Lett., 53 (14), pp. 1383-1386
- Singh, J., Mittal, S.K., Whole cell based amperometric sensor with relative selectivity for zinc ions (2012) Anal. Methods, 4, pp. 1326-1331
- Soltmann, U., Böttcher, H., Utilization of sol-gel ceramics for the immobilization of living microorganisms (2008) J. Sol-Gel Sci. Technol., 48, pp. 66-72
- Sicard, C., Perullini, M., Spedalieri, C., Coradin, T., Brayner, R., Livage, J., Jobbagy, M., Bilmes, S.A., CeO 2 nanoparticles for the protection of photosynthetic organisms immobilized in silica gels (2011) Chem. Mater., 23, pp. 1374-1378
- Rechnitz, G.A., Ho, M.Y., Biosensors based on cell and tissue material (1990) J. Biotechnol., 15, pp. 201-218
- Rogers, K.R., Biosensors for environmental application (1995) Biosens. Bioelectron., 10, pp. 533-541
- http://rsb.info.nih.gov/ij/download.html; Rachlin, J.W., Grosso, A., The effects of pH on the growth of Chlorella vulgaris and its interactions with cadmium toxicity (1991) Arch. Environ. Contam. Toxicol., 20 (4), pp. 505-508
- Zarzycki, J., Fractal properties of gels (1987) J. Non-Cryst. Solids, (1), pp. 173-184
Citas:
---------- APA ----------
Perullini, M., Ferro, Y., Durrieu, C., Jobbágy, M. & Bilmes, S.A.
(2014)
. Sol-gel silica platforms for microalgae-based optical biosensors. Journal of Biotechnology, 179(1), 65-70.
http://dx.doi.org/10.1016/j.jbiotec.2014.02.007---------- CHICAGO ----------
Perullini, M., Ferro, Y., Durrieu, C., Jobbágy, M., Bilmes, S.A.
"Sol-gel silica platforms for microalgae-based optical biosensors"
. Journal of Biotechnology 179, no. 1
(2014) : 65-70.
http://dx.doi.org/10.1016/j.jbiotec.2014.02.007---------- MLA ----------
Perullini, M., Ferro, Y., Durrieu, C., Jobbágy, M., Bilmes, S.A.
"Sol-gel silica platforms for microalgae-based optical biosensors"
. Journal of Biotechnology, vol. 179, no. 1, 2014, pp. 65-70.
http://dx.doi.org/10.1016/j.jbiotec.2014.02.007---------- VANCOUVER ----------
Perullini, M., Ferro, Y., Durrieu, C., Jobbágy, M., Bilmes, S.A. Sol-gel silica platforms for microalgae-based optical biosensors. J. Biotechnol. 2014;179(1):65-70.
http://dx.doi.org/10.1016/j.jbiotec.2014.02.007