Artículo

Perullini, M.; Ferro, Y.; Durrieu, C.; Jobbágy, M.; Bilmes, S.A. "Sol-gel silica platforms for microalgae-based optical biosensors" (2014) Journal of Biotechnology. 179(1):65-70
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Abstract:

An advanced hybrid biosensing platform with improved optical quality is developed based on the acidic encapsulation of microalgi in silica matrices synthesized by TAFR (tetraethoxysilane derived alcohol free route). The three microalgi (Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii) were previously immobilized in alginate following the two-step procedure. Tuning the alginate protecting function with the aid of Tris-HCl buffer, the sol-gel synthesis was conducted at pH 4.0 well below the tolerance limit imposed by the encapsulated microalgae. The acidic condensation of Si(IV) generates silica matrices with outstanding optical properties that suit the requirements of biosensors based on optical detection methods. © 2014 Elsevier B.V.

Registro:

Documento: Artículo
Título:Sol-gel silica platforms for microalgae-based optical biosensors
Autor:Perullini, M.; Ferro, Y.; Durrieu, C.; Jobbágy, M.; Bilmes, S.A.
Filiación:INQUIMAE-DQIAQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. Ciudad Universitaria, Pab. II, C1428EHA Buenos Aires, Argentina
Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés, UMR 5023 ENTPE, Université de Lyon, France
Palabras clave:Biosensor; Esterase activity; Microalgae; Optical quality; Sol-gel; Algae; Microorganisms; Silica; Sol-gel process; Sol-gels; Biosensing platforms; Chlamydomonas reinhardtii; Esterase activities; Micro-algae; Optical bio-sensors; Optical detection methods; Optical qualities; Pseudokirchneriella subcapitata; Biosensors; alginic acid; calcium alginate; silicate; silicon dioxide; tetraethoxysilane; esterase; glucuronic acid; hexuronic acid; silica gel; water pollutant; algal growth; article; biocompatibility; Chlamydomonas reinhardtii; Chlorella vulgaris; elementary particle; encapsulation; fluorescence; gelation; growth inhibition; hydrogel; nonhuman; optical biosensor; pH; polymerization; priority journal; Selenastrum capricornutum; synthesis; chemistry; enzymology; genetic procedures; isolation and purification; metabolism; microalga; procedures; small angle scattering; water pollutant; X ray diffraction; Alginates; Biosensing Techniques; Esterases; Glucuronic Acid; Hexuronic Acids; Microalgae; Scattering, Small Angle; Silica Gel; Water Pollutants, Chemical; X-Ray Diffraction
Año:2014
Volumen:179
Número:1
Página de inicio:65
Página de fin:70
DOI: http://dx.doi.org/10.1016/j.jbiotec.2014.02.007
Título revista:Journal of Biotechnology
Título revista abreviado:J. Biotechnol.
ISSN:01681656
CODEN:JBITD
CAS:alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; calcium alginate, 9005-35-0; silicate, 12627-13-3; silicon dioxide, 10279-57-9, 14464-46-1, 14808-60-7, 15468-32-3, 60676-86-0, 7631-86-9; esterase, 9013-79-0; glucuronic acid, 36116-79-7, 576-37-4, 6556-12-3; silica gel, 63231-67-4; Alginates; alginic acid; Esterases; Glucuronic Acid; Hexuronic Acids; Silica Gel; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v179_n1_p65_Perullini

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Citas:

---------- APA ----------
Perullini, M., Ferro, Y., Durrieu, C., Jobbágy, M. & Bilmes, S.A. (2014) . Sol-gel silica platforms for microalgae-based optical biosensors. Journal of Biotechnology, 179(1), 65-70.
http://dx.doi.org/10.1016/j.jbiotec.2014.02.007
---------- CHICAGO ----------
Perullini, M., Ferro, Y., Durrieu, C., Jobbágy, M., Bilmes, S.A. "Sol-gel silica platforms for microalgae-based optical biosensors" . Journal of Biotechnology 179, no. 1 (2014) : 65-70.
http://dx.doi.org/10.1016/j.jbiotec.2014.02.007
---------- MLA ----------
Perullini, M., Ferro, Y., Durrieu, C., Jobbágy, M., Bilmes, S.A. "Sol-gel silica platforms for microalgae-based optical biosensors" . Journal of Biotechnology, vol. 179, no. 1, 2014, pp. 65-70.
http://dx.doi.org/10.1016/j.jbiotec.2014.02.007
---------- VANCOUVER ----------
Perullini, M., Ferro, Y., Durrieu, C., Jobbágy, M., Bilmes, S.A. Sol-gel silica platforms for microalgae-based optical biosensors. J. Biotechnol. 2014;179(1):65-70.
http://dx.doi.org/10.1016/j.jbiotec.2014.02.007