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Perullini, M.; Rivero, M.M.; Jobbágy, M.; Mentaberry, A.; Bilmes, S.A. "Plant cell proliferation inside an inorganic host" (2007) Journal of Biotechnology. 127(3):542-548
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Abstract:

In recent years, much attention has been paid to plant cell culture as a tool for the production of secondary metabolites and the expression of recombinant proteins. Plant cell immobilization offers many advantages for biotechnological processes. However, the most extended matrices employed, such as calcium-alginate, cannot fully protect entrapped cells. Sol-gel chemistry of silicates has emerged as an outstanding strategy to obtain biomaterials in which living cells are truly protected. This field of research is rapidly developing and a large number of bacteria and yeast-entrapping ceramics have already been designed for different applications. But even mild thermal and chemical conditions employed in sol-gel synthesis may result harmful to cells of higher organisms. Here we present a method for the immobilization of plant cells that allows cell growth at cavities created inside a silica matrix. Plant cell proliferation was monitored for a 6-month period, at the end of which plant calli of more than 1 mm in diameter were observed inside the inorganic host. The resulting hybrid device had good mechanical stability and proved to be an effective barrier against biological contamination, suggesting that it could be employed for long-term plant cell entrapment applications. © 2006 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Plant cell proliferation inside an inorganic host
Autor:Perullini, M.; Rivero, M.M.; Jobbágy, M.; Mentaberry, A.; Bilmes, S.A.
Filiación:INQUIMAE-DQIAQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II, C1428EHA Buenos Aires, Argentina
Laboratorio de Agrobiotecnología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II, C1428EHA Buenos Aires, Argentina
Palabras clave:Plant cell immobilization; Silica matrix; Sol-gel; Bacteria; Plant cell culture; Plants (botany); Silica; Sol-gels; Biological contamination; Plant cell immobilization; Silica matrix; Sol-gel chemistry; Biotechnology; analytic method; article; cell growth; cell proliferation; contamination; extracellular matrix; host; immobilized cell; microbial contamination; nonhuman; plant cell; priority journal; Cell Culture Techniques; Cell Proliferation; Cells, Immobilized; Phloem; Silicon Dioxide; Tobacco
Año:2007
Volumen:127
Número:3
Página de inicio:542
Página de fin:548
DOI: http://dx.doi.org/10.1016/j.jbiotec.2006.07.024
Título revista:Journal of Biotechnology
Título revista abreviado:J. Biotechnol.
ISSN:01681656
CODEN:JBITD
CAS:Silicon Dioxide, 7631-86-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v127_n3_p542_Perullini

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Citas:

---------- APA ----------
Perullini, M., Rivero, M.M., Jobbágy, M., Mentaberry, A. & Bilmes, S.A. (2007) . Plant cell proliferation inside an inorganic host. Journal of Biotechnology, 127(3), 542-548.
http://dx.doi.org/10.1016/j.jbiotec.2006.07.024
---------- CHICAGO ----------
Perullini, M., Rivero, M.M., Jobbágy, M., Mentaberry, A., Bilmes, S.A. "Plant cell proliferation inside an inorganic host" . Journal of Biotechnology 127, no. 3 (2007) : 542-548.
http://dx.doi.org/10.1016/j.jbiotec.2006.07.024
---------- MLA ----------
Perullini, M., Rivero, M.M., Jobbágy, M., Mentaberry, A., Bilmes, S.A. "Plant cell proliferation inside an inorganic host" . Journal of Biotechnology, vol. 127, no. 3, 2007, pp. 542-548.
http://dx.doi.org/10.1016/j.jbiotec.2006.07.024
---------- VANCOUVER ----------
Perullini, M., Rivero, M.M., Jobbágy, M., Mentaberry, A., Bilmes, S.A. Plant cell proliferation inside an inorganic host. J. Biotechnol. 2007;127(3):542-548.
http://dx.doi.org/10.1016/j.jbiotec.2006.07.024