Artículo
Salamone, D.; Barañao, L.; Santos, C.; Bussmann, L.; Artuso, J.; Werning, C.; Prync, A.; Carbonetto, C.; Dabsys, S.; Munar, C.; Salaberry, R.; Berra, G.; Berra, I.; Fernández, N.; Papouchado, M.; Foti, M.; Judewicz, N.; Mujica, I. (...) Melo, C. "High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow" (2006) Journal of Biotechnology. 124(2):469-472
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Abstract:
Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels of up to 5 g l-1. The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological and somatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable of producing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for the production of this hormone. © 2006.
Registro:
| Documento: | Artículo |
| Título: | High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow |
| Autor: | Salamone, D.; Barañao, L.; Santos, C.; Bussmann, L.; Artuso, J.; Werning, C.; Prync, A.; Carbonetto, C.; Dabsys, S.; Munar, C.; Salaberry, R.; Berra, G.; Berra, I.; Fernández, N.; Papouchado, M.; Foti, M.; Judewicz, N.; Mujica, I.; Muñoz, L.; Alvarez, S.F.; González, E.; Zimmermann, J.; Criscuolo, M.; Melo, C. |
| Filiación: | Biosidus S.A., Buenos Aires, Argentina Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, Argentina Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Instituto de Biología y Medicina Experimental-CONICET, Argentina Instituto Nacional de Tecnología Agropecuaria, Argentina Munar y Asociados, Pcia de Buenos Aires, Buenos Aires, Argentina Centro de Diagnóstico Molecular S.A., Buenos Aires, Argentina |
| Palabras clave: | Animal biotechnology; Recombinant hGH; Transgenic cloned cow; Biotechnology; Cost effectiveness; Dairy products; Drug products; Escherichia coli; Animal biotechnology; Recombinant hGH; Transgenic cloned cow; Hormones; human growth hormone; animal cell; animal experiment; article; breed; cattle; clone; cost effectiveness analysis; cow; Escherichia coli; fertility; fetus; hematological parameters; hormone synthesis; milk; nonhuman; priority journal; progeny; transgenic animal; Animals; Animals, Genetically Modified; Cattle; Cloning, Organism; Human Growth Hormone; Humans; Milk Proteins; Recombinant Proteins; Animalia; Bos taurus |
| Año: | 2006 |
| Volumen: | 124 |
| Número: | 2 |
| Página de inicio: | 469 |
| Página de fin: | 472 |
| DOI: | http://dx.doi.org/10.1016/j.jbiotec.2006.01.005 |
| Título revista: | Journal of Biotechnology |
| Título revista abreviado: | J. Biotechnol. |
| ISSN: | 01681656 |
| CODEN: | JBITD |
| CAS: | human growth hormone, 12629-01-5; Human Growth Hormone, 12629-01-5; Milk Proteins; Recombinant Proteins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v124_n2_p469_Salamone |
Referencias:
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Citas:
---------- APA ----------
Salamone, D., Barañao, L., Santos, C., Bussmann, L., Artuso, J., Werning, C., Prync, A.,..., Melo, C. (2006) . High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow. Journal of Biotechnology, 124(2), 469-472.http://dx.doi.org/10.1016/j.jbiotec.2006.01.005
---------- CHICAGO ----------
Salamone, D., Barañao, L., Santos, C., Bussmann, L., Artuso, J., Werning, C., et al. "High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow" . Journal of Biotechnology 124, no. 2 (2006) : 469-472.http://dx.doi.org/10.1016/j.jbiotec.2006.01.005
---------- MLA ----------
Salamone, D., Barañao, L., Santos, C., Bussmann, L., Artuso, J., Werning, C., et al. "High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow" . Journal of Biotechnology, vol. 124, no. 2, 2006, pp. 469-472.http://dx.doi.org/10.1016/j.jbiotec.2006.01.005
---------- VANCOUVER ----------
Salamone, D., Barañao, L., Santos, C., Bussmann, L., Artuso, J., Werning, C., et al. High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow. J. Biotechnol. 2006;124(2):469-472.http://dx.doi.org/10.1016/j.jbiotec.2006.01.005
