Artículo
López-Malo, A.; Alzamora, S.M.; Palou, E. "Aspergillus flavus dose-response curves to selected natural and synthetic antimicrobials" (2002) International Journal of Food Microbiology. 73(2-3):213-218
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Abstract:
The effects of selected concentrations of antimicrobials from natural (vanillin, thymol, eugenol, carvacrol or citral) or synthetic (potassium sorbate or sodium benzoate) origin on Aspergillus flavus lag time inoculated in laboratory media formulated at water activity (aw) 0.99 and pH 4.5 or 3.5, were evaluated. Time to detect a colony with a diameter >0.5 mm was determined. Mold response was modeled using the Fermi function. Antimicrobial minimal inhibitory concentration (MIC) was defined as the minimal required inhibiting mold growth for 2 months. Fermi function successfully captured A. flavus dose-response curves to the tested antimicrobials with a highly satisfactory fit. Fermi equation coefficients, Pc and k, were used to compare antimicrobials and assess the effect of pH. Important differences in Pc and k were observed among antimicrobials, being natural antimicrobials less pH dependent than synthetic antimicrobials. A large Pc value represents a small antimicrobial effect on A. flavus lag time; thus, high concentrations are needed to delay growth. A. flavus exhibited higher sensitivity to thymol, eugenol, carvacrol, potassium sorbate (at pH 3.5), and sodium benzoate (at pH 3.5) than to vanillin or citral. MICs varied from 200 ppm of sodium benzoate at pH 3.5 to 1800 ppm of citral at both evaluated pHs. © 2002 Elsevier Science B.V. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | Aspergillus flavus dose-response curves to selected natural and synthetic antimicrobials |
| Autor: | López-Malo, A.; Alzamora, S.M.; Palou, E. |
| Filiación: | Departamento de Ingeniería Química y Alimentos, Universidad de Las Américas-Puebla, Cholula 72820, Mexico Departamento de Industrias, FCEyN, Universidad de Buenos Aires, Ciudad Universitaria, Capital Federal, Buenos Aires, Argentina |
| Palabras clave: | Antimicrobials; Aspergillus flavus; Dose-response; antiinfective agent; benzoic acid; carvacrol; citral; eugenol; sorbate potassium; thymol; vanillin; water; antimicrobial activity; article; Aspergillus flavus; concentration response; controlled study; drug effect; drug response; fungus growth; growth inhibition; minimum inhibitory concentration; mould; nonhuman; pH; Anti-Infective Agents; Aspergillus flavus; Colony Count, Microbial; Dose-Response Relationship, Drug; Food Preservatives; Hydrogen-Ion Concentration; Microbial Sensitivity Tests; Sorbic Acid; Thymol; Time Factors; Water; Aspergillus; Aspergillus flavus |
| Año: | 2002 |
| Volumen: | 73 |
| Número: | 2-3 |
| Página de inicio: | 213 |
| Página de fin: | 218 |
| DOI: | http://dx.doi.org/10.1016/S0168-1605(01)00639-0 |
| Título revista: | International Journal of Food Microbiology |
| Título revista abreviado: | Int. J. Food Microbiol. |
| ISSN: | 01681605 |
| CODEN: | IJFMD |
| CAS: | Anti-Infective Agents; Food Preservatives; Sorbic Acid, 110-44-1; Thymol, 89-83-8; Water, 7732-18-5 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v73_n2-3_p213_LopezMalo |
Referencias:
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Citas:
---------- APA ----------
López-Malo, A., Alzamora, S.M. & Palou, E. (2002) . Aspergillus flavus dose-response curves to selected natural and synthetic antimicrobials. International Journal of Food Microbiology, 73(2-3), 213-218.http://dx.doi.org/10.1016/S0168-1605(01)00639-0
---------- CHICAGO ----------
López-Malo, A., Alzamora, S.M., Palou, E. "Aspergillus flavus dose-response curves to selected natural and synthetic antimicrobials" . International Journal of Food Microbiology 73, no. 2-3 (2002) : 213-218.http://dx.doi.org/10.1016/S0168-1605(01)00639-0
---------- MLA ----------
López-Malo, A., Alzamora, S.M., Palou, E. "Aspergillus flavus dose-response curves to selected natural and synthetic antimicrobials" . International Journal of Food Microbiology, vol. 73, no. 2-3, 2002, pp. 213-218.http://dx.doi.org/10.1016/S0168-1605(01)00639-0
---------- VANCOUVER ----------
López-Malo, A., Alzamora, S.M., Palou, E. Aspergillus flavus dose-response curves to selected natural and synthetic antimicrobials. Int. J. Food Microbiol. 2002;73(2-3):213-218.http://dx.doi.org/10.1016/S0168-1605(01)00639-0
