Artículo
Beuchat, L.R; Frändberg, E.; Deak, T.; Alzamora, S.M.; Chen, J.; Guerrero, S.; López-Malo, A.; Ohlsson, I.; Olsen, M.; Peinado, J.M.; Schnurer, J.; De Siloniz, M.I.; Tornai-Lehoczki, J. "Performance of mycological media in enumerating desiccated food spoilage yeasts: An interlaboratory study" (2001) International Journal of Food Microbiology. 70(1-2):89-96
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Abstract:
Dichloran 18% glycerol agar (DG18) was originally formulated to enumerate nonfastidious xerophilic moulds in foods containing rapidly growing Eurotium species. Some laboratories are now using DG18 as a general purpose medium for enumerating yeasts and moulds, although its performance in recovering yeasts from dry foods has not been evaluated. An interlaboratory study compared DG18 with dichloran rose bengal chloramphenicol agar (DRBC), plate count agar supplemented with chloramphenicol (PCAC), tryptone glucose yeast extract chloramphenicol agar (TGYC), acidified potato dextrose agar (APDA), and orange serum agar (OSA) for their suitability to enumerate 14 species of lyophilized yeasts. The coefficient of variation for among-laboratories repeatability within yeast was 1.39% and reproducibility of counts among laboratories was 7.1%. The order of performance of media for recovering yeasts was TGYC>PCAC=OSA>APDA>DRBC>DG18. A second study was done to determine the combined effects of storage time and temperature on viability of yeasts and suitability of media for recovery. Higher viability was retained at -18°C than at 5°C or 25°C for up to 42 weeks, although the difference in mean counts of yeasts stored at -18°C and 25°C was only 0.78 log10 cfu/ml of rehydrated suspension. TGYC was equal to PCAC and superior to the other four media in recovering yeasts stored at -18°C, 5°C, or 25°C for up to 42 weeks. Results from both the interlaboratory study and the storage study support the use of TGYC for enumerating desiccated yeasts. DG18 is not recommended as a general purpose medium for recovering yeasts from a desiccated condition. Copyright © 2001 Elsevier Science B.V.
Registro:
| Documento: | Artículo |
| Título: | Performance of mycological media in enumerating desiccated food spoilage yeasts: An interlaboratory study |
| Autor: | Beuchat, L.R; Frändberg, E.; Deak, T.; Alzamora, S.M.; Chen, J.; Guerrero, S.; López-Malo, A.; Ohlsson, I.; Olsen, M.; Peinado, J.M.; Schnurer, J.; De Siloniz, M.I.; Tornai-Lehoczki, J. |
| Filiación: | Center for Food Safety and Department of Food Science and Technology, University of Georgia, 1109 Experiment Street, Griffin, GA 30223-1797, United States Biology Division, National Food Administration, 751 26 Uppsala, Sweden Department of Microbiology and Biotechnology, Szent Istvan University, Somloi Ut 14-16, 1118 Budapest, Hungary Pabellón de Industrias, Fac. de Cie. Exact. y Nat., Univ. de Buenos Aires, 1428 Buenos Aires, Argentina Departmento de Ingenieria Quimica y Alimentos, Universidad de la Americas-Puebla, Sta. Catarina Mártir, Puebla 72820, Mexico Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, 750 07 Uppsala, Sweden Departmento de Microbiologia, Facultad de Biologia, Universidad Completense de Madrid, 20840 Madrid, Spain National Collection of Agricultural and Industrial Microorganisms, Szent Istvan University, Somloi Ut 14-16, 1118 Budapest, Hungary |
| Palabras clave: | Mycological media; TGYC; Yeast; chloramphenicol; glucose; peptone; rose bengal; article; culture medium; desiccation; food spoilage; freeze drying; fungus growth; milk; mould; nonhuman; storage; temperature; yeast; Agar; Colony Count, Microbial; Culture Media; Food Contamination; Food Microbiology; Microbiological Techniques; Reproducibility of Results; Sensitivity and Specificity; Temperature; Time Factors; Water; Yeasts |
| Año: | 2001 |
| Volumen: | 70 |
| Número: | 1-2 |
| Página de inicio: | 89 |
| Página de fin: | 96 |
| DOI: | http://dx.doi.org/10.1016/S0168-1605(01)00519-0 |
| Título revista: | International Journal of Food Microbiology |
| Título revista abreviado: | Int. J. Food Microbiol. |
| ISSN: | 01681605 |
| CODEN: | IJFMD |
| CAS: | Agar, 9002-18-0; Culture Media; Water, 7732-18-5 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v70_n1-2_p89_Beuchat |
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Citas:
---------- APA ----------
Beuchat, L.R, Frändberg, E., Deak, T., Alzamora, S.M., Chen, J., Guerrero, S., López-Malo, A.,..., Tornai-Lehoczki, J. (2001) . Performance of mycological media in enumerating desiccated food spoilage yeasts: An interlaboratory study. International Journal of Food Microbiology, 70(1-2), 89-96.http://dx.doi.org/10.1016/S0168-1605(01)00519-0
---------- CHICAGO ----------
Beuchat, L.R, Frändberg, E., Deak, T., Alzamora, S.M., Chen, J., Guerrero, S., et al.. "Performance of mycological media in enumerating desiccated food spoilage yeasts: An interlaboratory study" . International Journal of Food Microbiology 70, no. 1-2 (2001) : 89-96.http://dx.doi.org/10.1016/S0168-1605(01)00519-0
---------- MLA ----------
Beuchat, L.R, Frändberg, E., Deak, T., Alzamora, S.M., Chen, J., Guerrero, S., et al.. "Performance of mycological media in enumerating desiccated food spoilage yeasts: An interlaboratory study" . International Journal of Food Microbiology, vol. 70, no. 1-2, 2001, pp. 89-96.http://dx.doi.org/10.1016/S0168-1605(01)00519-0
---------- VANCOUVER ----------
Beuchat, L.R, Frändberg, E., Deak, T., Alzamora, S.M., Chen, J., Guerrero, S., et al.. Performance of mycological media in enumerating desiccated food spoilage yeasts: An interlaboratory study. Int. J. Food Microbiol. 2001;70(1-2):89-96.http://dx.doi.org/10.1016/S0168-1605(01)00519-0
