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Alternaria spp. are major contaminants of wheat crops, causing both economic losses for producers and health risk for consumers due to the accumulation of toxic metabolites. The application of synthetic fungicides in the field may trigger mycotoxin accumulation, since fungistatic levels of those compounds might cause fungal responses to stress. Hence, new alternatives are needed for its control. The aim of this work was to compare the effects of a natural antifungal compound, the antifungal protein PgAFP, and a synthetic commercial one, on Alternaria tenuissima sp.-grp. growth, mycotoxin biosynthesis (tenuazonic acid and alternariols) and the expression of a stress-related gene associated with cell wall integrity (CWI) pathway, in a wheat-based medium at two water activities (a w ; 0.95 and 0.98 a w ) conditions associated with the ripening of this grain. The application of both antifungals produced comparable fungistatic effects on Alternaria spp. growth. However, the presence of PgAFP produced a significant reduction in mycotoxins accumulation, whereas this effect was not observed with the commercial antifungal. To our knowledge, this is the first study on the influence of fungicides on the expression of a key gene involved in CWI stress-related pathway in relation to Alternaria mycotoxins accumulation. This information is useful when developing new antifungal methods for foods. The application of PgAFP would be a promising natural strategy for its application in wheat for the control of Alternaria spp. © 2018 Elsevier B.V.


Documento: Artículo
Título:Differential response to synthetic and natural antifungals by Alternaria tenuissima in wheat simulating media: Growth, mycotoxin production and expression of a gene related to cell wall integrity
Autor:da Cruz Cabral, L.; Delgado, J.; Patriarca, A.; Rodríguez, A.
Filiación:Food Hygiene and Safety, Meat and Meat Products Research Institute, Faculty of Veterinary Science, University of Extremadura, Avda. de las Ciencias, s/n., Cáceres, 10003, Spain
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica, Laboratorio de Microbiología de Alimentos, Buenos Aires, Argentina
Palabras clave:Alternariols; Antifungal protein; Cereals; Control; CWI; Tenuazonic acid; alternariol; complementary DNA; fungal DNA; fungal protein; fungicide; genomic DNA; mycotoxin; pgafp protein; tenuazonic acid; unclassified drug; alternariol; antifungal agent; fungal protein; fungicide; lactone; mycotoxin; tenuazonic acid; water; Alternaria; Alternaria tenuissima; Article; comparative study; controlled study; fungal cell wall; fungal gene; fungal strain; fungicidal activity; fungus growth; fungus spore; gene expression; gene repression; grain; nonhuman; Penicillium chrysogenum; Rho1 gene; spore germination; stress; toxin synthesis; Triticum aestivum; wheat; Alternaria; biosynthesis; cell wall; growth, development and aging; metabolism; microbiology; pathogenicity; wheat; Alternaria; Antifungal Agents; Cell Wall; Fungal Proteins; Fungicides, Industrial; Lactones; Mycotoxins; Penicillium chrysogenum; Tenuazonic Acid; Triticum; Water
Página de inicio:48
Página de fin:55
Título revista:International Journal of Food Microbiology
Título revista abreviado:Int. J. Food Microbiol.
CAS:alternariol, 641-38-3; tenuazonic acid, 610-88-8, 75652-74-3; lactone, 1338-03-0; water, 7732-18-5; alternariol; Antifungal Agents; Fungal Proteins; Fungicides, Industrial; Lactones; Mycotoxins; Tenuazonic Acid; Water


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---------- APA ----------
da Cruz Cabral, L., Delgado, J., Patriarca, A. & Rodríguez, A. (2019) . Differential response to synthetic and natural antifungals by Alternaria tenuissima in wheat simulating media: Growth, mycotoxin production and expression of a gene related to cell wall integrity. International Journal of Food Microbiology, 292, 48-55.
---------- CHICAGO ----------
da Cruz Cabral, L., Delgado, J., Patriarca, A., Rodríguez, A. "Differential response to synthetic and natural antifungals by Alternaria tenuissima in wheat simulating media: Growth, mycotoxin production and expression of a gene related to cell wall integrity" . International Journal of Food Microbiology 292 (2019) : 48-55.
---------- MLA ----------
da Cruz Cabral, L., Delgado, J., Patriarca, A., Rodríguez, A. "Differential response to synthetic and natural antifungals by Alternaria tenuissima in wheat simulating media: Growth, mycotoxin production and expression of a gene related to cell wall integrity" . International Journal of Food Microbiology, vol. 292, 2019, pp. 48-55.
---------- VANCOUVER ----------
da Cruz Cabral, L., Delgado, J., Patriarca, A., Rodríguez, A. Differential response to synthetic and natural antifungals by Alternaria tenuissima in wheat simulating media: Growth, mycotoxin production and expression of a gene related to cell wall integrity. Int. J. Food Microbiol. 2019;292:48-55.