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Abstract:

Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a gliotoxinogenic species into section Fumigati commonly found in contaminated animal environments. In dairy herds, exposed areas of lactating cows, as mammalian glandule, can be easily contaminated by them. This study was aimed to identify A. fumigatus sensu lato strains (identified based on morphology) isolated from raw cow milk at species level, by morphological and molecular techniques, and to estimate their genetic variability. Forty-five A. fumigatus strains showed similar RAPD profiles (generated with PELF and URP1F primers) to each other and to A. fumigatus sensu stricto reference strains; also, they were almost identical to clinical human and feed-borne A. fumigatus strains included in the assay, since their similarity coefficient ranged from 0.7 to 1.00. Therefore, all strains were characterized as belonging to A. fumigatus sensu stricto species. This result was supported by sequencing the benA gene of selected strains and by maximum parsimony analysis. In addition, RAPD fingerprinting demonstrated intra-specific genetic variability into the A. fumigatus sensu stricto cluster. The results found in this study strengthen the fact that A. fumigatus sensu stricto is the predominant species in the Aspergillus section Fumigati found in animal environments such as dairy herd environments, while other species such as A. novofumigatus, A. fumigatiaffinis, A. udagawae and A. lentulus may be rarely isolated. Since no differences between animal and human strains were observed they can become pathogenic also for farm handlers’. Moreover, the presence of A. fumigatus sensu stricto in raw cow milk is probably a very important risk factor since milk and its by-products are generally indented for human consumption, then gliotoxin could be transferred to them. © 2018

Registro:

Documento: Artículo
Título:Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina: Molecular typing of A. fumigatus from raw cow milk
Autor:Pena, G.A.; Alonso, V.; Manini, M.V.; Pellegrino, M.; Cavaglieri, L.R.
Filiación:Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36 Km. 601, Río Cuarto, Córdoba, Argentina
Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Rivadavia 1917, Buenos Aires, Argentina
Palabras clave:A. fumigatus sensu stricto; Genetic variability estimation; Molecular typing; Raw cow milk; gliotoxin; Argentina; Article; Aspergillus fumigatus; cluster analysis; fungal strain; fungus isolation; genetic distance; genetic variability; milk; molecular typing; nonhuman; parsimony analysis; random amplified polymorphic DNA; raw food
Año:2018
Volumen:275
Página de inicio:1
Página de fin:7
DOI: http://dx.doi.org/10.1016/j.ijfoodmicro.2018.03.018
Título revista:International Journal of Food Microbiology
Título revista abreviado:Int. J. Food Microbiol.
ISSN:01681605
CODEN:IJFMD
CAS:gliotoxin, 37273-88-4, 67-99-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v275_n_p1_Pena

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Citas:

---------- APA ----------
Pena, G.A., Alonso, V., Manini, M.V., Pellegrino, M. & Cavaglieri, L.R. (2018) . Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina: Molecular typing of A. fumigatus from raw cow milk. International Journal of Food Microbiology, 275, 1-7.
http://dx.doi.org/10.1016/j.ijfoodmicro.2018.03.018
---------- CHICAGO ----------
Pena, G.A., Alonso, V., Manini, M.V., Pellegrino, M., Cavaglieri, L.R. "Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina: Molecular typing of A. fumigatus from raw cow milk" . International Journal of Food Microbiology 275 (2018) : 1-7.
http://dx.doi.org/10.1016/j.ijfoodmicro.2018.03.018
---------- MLA ----------
Pena, G.A., Alonso, V., Manini, M.V., Pellegrino, M., Cavaglieri, L.R. "Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina: Molecular typing of A. fumigatus from raw cow milk" . International Journal of Food Microbiology, vol. 275, 2018, pp. 1-7.
http://dx.doi.org/10.1016/j.ijfoodmicro.2018.03.018
---------- VANCOUVER ----------
Pena, G.A., Alonso, V., Manini, M.V., Pellegrino, M., Cavaglieri, L.R. Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina: Molecular typing of A. fumigatus from raw cow milk. Int. J. Food Microbiol. 2018;275:1-7.
http://dx.doi.org/10.1016/j.ijfoodmicro.2018.03.018