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Abstract:

Alternaria species have the ability to produce a variety of secondary metabolite, which plays important roles in food safety. Argentina is the second largest exporter of fresh and processed food products to Europe, however, few studies on Alternaria mycotoxins and other bioactive secondary metabolites have been carried out on Argentinean cereals, fruit and vegetables. Knowing the full chemical potential and the distribution of Alternaria spp. on crops, it is necessary to establish a toxicological risk assessment for food products for human consumption. In the present study, 87 Alternaria strains from different substrates (tomato, wheat, blueberries and walnuts) were characterized according to morphology and metabolite production. Aggressive dereplication (accurate mass, isotopic patterns and lists of all described compounds from Alternaria) was used for high-throughput evaluation of the chemical potential. Four strains belonged to the Alternaria infectoria sp.-grp., 6 to the Alternaria arborescens sp.-grp., 6 showed a sporulation pattern similar to that of "M" according to Simmons, 1 to that of Alternaria vaccinii, and the remaining 70 constituted a diverse group belonging to morphological groups "G" and "H". The cluster analysis yielded 16 almost identical dendrograms and grouped the Alternaria strains into four clusters and 11 singletons and outlier groups. The chemical analysis showed that AOH and AME were the most common metabolites produced, followed by TEN, ALXs and TeA. The A. infectoria sp.-grp. had no metabolites in common with the rest of the strains. Several secondary metabolites isolated from large-spored Alternaria species or other fungal genera were detected, such as dehydrocurvularin, pyrenochaetic acid and alternarienonic acid. The strains isolated from tomato produced lower amounts of metabolites than strains from blueberries, walnut and wheat, although individual strains from tomato produced the highest amount of some metabolites. The A. infectoria sp.-grp. was unique to cereals, whereas strains classified as belonging to the A. arborescens sp.-grp or having sporulation pattern "M" were only isolated from tomatoes. Otherwise, no clear association between substrate and identity could be found. The analyses in the study show that at least 75% of the Argentinean strains are able to produce potential mycotoxins. © 2014 Elsevier B.V.

Registro:

Documento: Artículo
Título:Characterization of Alternaria strains from Argentinean blueberry, tomato, walnut and wheat
Autor:Andersen, B.; Nielsen, K.F.; Fernández Pinto, V.; Patriarca, A.
Filiación:Department of Systems Biology, Søltofts Plads, Technical University of Denmark, Kgs. Lyngby, DK-2800, Denmark
Laboratorio de Microbiología de Alimentos, Departamento de Química Orgánica, Ciudad Universitaria, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Pabellón II, 3 Piso, Buenos Aires, C1428EGA, Argentina
Palabras clave:Alternaria; Metabolite profiling; Morphology; alpha,beta dehydrocurvularin; alternarienonic acid; mycotoxin; pyrenochaetic acid; toxin; unclassified drug; Alternaria; Article; blueberry; chemical analysis; mass; metabolite; morphology; nonhuman; phylogenetic tree; sporogenesis; tomato; walnut; wheat; Alternaria; Argentina; blueberry; chemistry; classification; food control; fruit; genetics; hazard assessment; isolation and purification; microbiology; nut; physiology; tomato; walnut; wheat; Alternaria; Alternaria arborescens; Juglans; Lewia infectoria; Lycopersicon esculentum; Triticum aestivum; Vaccinium; Alternaria; Argentina; Blueberry Plant; Food Microbiology; Fruit; Hazard Analysis and Critical Control Points; Juglans; Lycopersicon esculentum; Mycotoxins; Nuts; Triticum
Año:2015
Volumen:196
Página de inicio:1
Página de fin:10
DOI: http://dx.doi.org/10.1016/j.ijfoodmicro.2014.11.029
Título revista:International Journal of Food Microbiology
Título revista abreviado:Int. J. Food Microbiol.
ISSN:01681605
CODEN:IJFMD
CAS:alpha,beta dehydrocurvularin, 21178-57-4; Mycotoxins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v196_n_p1_Andersen

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Citas:

---------- APA ----------
Andersen, B., Nielsen, K.F., Fernández Pinto, V. & Patriarca, A. (2015) . Characterization of Alternaria strains from Argentinean blueberry, tomato, walnut and wheat. International Journal of Food Microbiology, 196, 1-10.
http://dx.doi.org/10.1016/j.ijfoodmicro.2014.11.029
---------- CHICAGO ----------
Andersen, B., Nielsen, K.F., Fernández Pinto, V., Patriarca, A. "Characterization of Alternaria strains from Argentinean blueberry, tomato, walnut and wheat" . International Journal of Food Microbiology 196 (2015) : 1-10.
http://dx.doi.org/10.1016/j.ijfoodmicro.2014.11.029
---------- MLA ----------
Andersen, B., Nielsen, K.F., Fernández Pinto, V., Patriarca, A. "Characterization of Alternaria strains from Argentinean blueberry, tomato, walnut and wheat" . International Journal of Food Microbiology, vol. 196, 2015, pp. 1-10.
http://dx.doi.org/10.1016/j.ijfoodmicro.2014.11.029
---------- VANCOUVER ----------
Andersen, B., Nielsen, K.F., Fernández Pinto, V., Patriarca, A. Characterization of Alternaria strains from Argentinean blueberry, tomato, walnut and wheat. Int. J. Food Microbiol. 2015;196:1-10.
http://dx.doi.org/10.1016/j.ijfoodmicro.2014.11.029