Artículo

Castañares, E.; Albuquerque, D.R.; Dinolfo, M.I.; Pinto, V.F.; Patriarca, A.; Stenglein, S.A. "Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina" (2014) International Journal of Food Microbiology. 179:57-63
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Abstract:

Fusarium graminearum is one of the most important pathogens isolated from small cereal grains with Fusarium Head Blight symptoms. The presence of this fungus is often linked to the occurrence of several mycotoxins in barley and wheat. The aim of our study was to characterize trichothecene genotypes and production profiles of F. graminearum sensu stricto isolates obtained from barley grains in Argentina. A total of 110 F. graminearum s.s. isolates were analyzed by PCR assays to predict deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-ADON), 3-acetyldeoxynivalenol (3-ADON) and nivalenol (NIV) production, and all isolates were found to belong to the same molecular 15-ADON genotype. Trichothecene production in autoclaved rice was analyzed by using gas chromatography (GC) and confirmed by GC-MS. Of the 110 isolates, 95% were able to produce DON, 71% produced 15-ADON, 63% 3-ADON and 52% NIV. With the exception of a single isolate, all isolates that produced NIV, also produced DON. However, the NIV production was very low, ranging from 0.13 to 0.30. μg/g. Six different production profiles of DON and its acetyl-derivatives were detected, the predominant being simultaneous production of DON, 3-ADON and 15-ADON, followed by DON production, and DON and 15-ADON co-production. This work is the first attempt to characterize the trichothecene genotypes and production profiles of F. graminearum s.s. isolates from Argentinean barley. © 2014 Elsevier B.V.

Registro:

Documento: Artículo
Título:Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina
Autor:Castañares, E.; Albuquerque, D.R.; Dinolfo, M.I.; Pinto, V.F.; Patriarca, A.; Stenglein, S.A.
Filiación:Laboratorio de Biología Funcional y Biotecnología (BIOLAB)-CICBA-INBIOTEC, Facultad de Agronomía de Azul-UNCPBA, Av. República de Italia 780, 7300 Azul, Buenos Aires, Argentina
Laboratorio de Microbiología de Alimentos, PROPLAME-PRHIDEB, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, UBA, C1428EGA Buenos Aires, Argentina
Cátedra de Microbiología, Facultad de Agronomía de Azul-UNCPBA, Av. República de Italia 780, 7300, Azul, Buenos Aires, Argentina
Palabras clave:Chemical production profiles; Fusarium graminearum; Genotype; Hordeum vulgare; Trichothecenes; 15 acetylvomitoxin; 3 acetyldeoxynivalenol; nivalenol; trichothecene; unclassified drug; vomitoxin; Argentina; article; barley; controlled study; Fusarium graminearum; gas chromatography; genotype; mass fragmentography; mycotoxicosis; nonhuman; Chemical production profiles; Fusarium graminearum; Genotype; Hordeum vulgare; Trichothecenes; Argentina; Fusarium; Genotype; Hordeum; Mycotoxins; Polymerase Chain Reaction
Año:2014
Volumen:179
Página de inicio:57
Página de fin:63
DOI: http://dx.doi.org/10.1016/j.ijfoodmicro.2014.03.024
Título revista:International Journal of Food Microbiology
Título revista abreviado:Int. J. Food Microbiol.
ISSN:01681605
CODEN:IJFMD
CAS:nivalenol, 23282-20-4; trichothecene, 51724-48-2; vomitoxin, 51481-10-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v179_n_p57_Castanares

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Citas:

---------- APA ----------
Castañares, E., Albuquerque, D.R., Dinolfo, M.I., Pinto, V.F., Patriarca, A. & Stenglein, S.A. (2014) . Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina. International Journal of Food Microbiology, 179, 57-63.
http://dx.doi.org/10.1016/j.ijfoodmicro.2014.03.024
---------- CHICAGO ----------
Castañares, E., Albuquerque, D.R., Dinolfo, M.I., Pinto, V.F., Patriarca, A., Stenglein, S.A. "Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina" . International Journal of Food Microbiology 179 (2014) : 57-63.
http://dx.doi.org/10.1016/j.ijfoodmicro.2014.03.024
---------- MLA ----------
Castañares, E., Albuquerque, D.R., Dinolfo, M.I., Pinto, V.F., Patriarca, A., Stenglein, S.A. "Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina" . International Journal of Food Microbiology, vol. 179, 2014, pp. 57-63.
http://dx.doi.org/10.1016/j.ijfoodmicro.2014.03.024
---------- VANCOUVER ----------
Castañares, E., Albuquerque, D.R., Dinolfo, M.I., Pinto, V.F., Patriarca, A., Stenglein, S.A. Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina. Int. J. Food Microbiol. 2014;179:57-63.
http://dx.doi.org/10.1016/j.ijfoodmicro.2014.03.024