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Abstract:

Growth rate, lag phase and OTA production of a mixed inoculum of four Aspergillus carbonarius strains were compared to the behaviour of the single strains at 30 °C on Czapek Yeast Extract Agar. Significant differences between radial growth rates and lag phases of the different isolates were observed; however, no significant differences between growth rates of each strain and the mixed inoculum were detected. When the four strains were inoculated simultaneously, the lag phase was the same than the higher value obtained for individual strains, suggesting mycelial interactions between the A. carbonarius isolates. The four strains differed in maximum OTA yield, and the toxin accumulation by the mixed inoculum showed intermediate levels at each time point. The effects of water activity (0.83, 0.85, 0.87, 0.89, 0.90, 0.93 and 0.95) and temperature (15, 20, 25, 30, and 35 °C) on OTA production by the mixed inoculum were studied at 7, 14, 21 and 28. days of incubation. The limiting water activity for OTA production by the mixed inoculum was 0.87, showing xerotolerant behaviour of the strains isolated from dried vine fruits. Results obtained were similar to those reported for single A. carbonarius strains from European countries, Israel, Australia and South America. The similar trend in the response of the different isolates to the variation of environmental parameters may be of interest for the building of predictive models. © 2010 Elsevier B.V.

Registro:

Documento: Artículo
Título:Ochratoxin A production by a mixed inoculum of Aspergillus carbonarius at different conditions of water activity and temperature
Autor:Romero, S.M.; Pinto, V.F.; Patriarca, A.; Vaamonde, G.
Filiación:Laboratorio de Microbiología de Alimentos, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II, 3 Piso (1428), Buenos Aires, Argentina
Palabras clave:Aspergillus carbonarius; Mixed inoculum; Ochratoxin A; Temperature; Water activity; ochratoxin; water; ochratoxin; article; Aspergillus carbonarius; Australia; controlled study; environmental parameters; fungal metabolism; fungal strain; fungus growth; fungus isolation; geographical variation (species); growth rate; inoculation; Israel; mixed cell culture; nonhuman; South America; temperature sensitivity; toxin synthesis; trend study; water flow; Aspergillus; growth, development and aging; metabolism; temperature; Aspergillus; Ochratoxins; Temperature; Water; Aspergillus carbonarius; Aspergillus; Ochratoxins; Temperature; Water
Año:2010
Volumen:140
Número:2-3
Página de inicio:277
Página de fin:281
DOI: http://dx.doi.org/10.1016/j.ijfoodmicro.2010.04.013
Título revista:International Journal of Food Microbiology
Título revista abreviado:Int. J. Food Microbiol.
ISSN:01681605
CODEN:IJFMD
CAS:ochratoxin, 303-47-9, 37203-43-3; water, 7732-18-5; Ochratoxins; Water, 7732-18-5; ochratoxin A, 303-47-9; ochratoxin A; Ochratoxins; Water
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v140_n2-3_p277_Romero

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Citas:

---------- APA ----------
Romero, S.M., Pinto, V.F., Patriarca, A. & Vaamonde, G. (2010) . Ochratoxin A production by a mixed inoculum of Aspergillus carbonarius at different conditions of water activity and temperature. International Journal of Food Microbiology, 140(2-3), 277-281.
http://dx.doi.org/10.1016/j.ijfoodmicro.2010.04.013
---------- CHICAGO ----------
Romero, S.M., Pinto, V.F., Patriarca, A., Vaamonde, G. "Ochratoxin A production by a mixed inoculum of Aspergillus carbonarius at different conditions of water activity and temperature" . International Journal of Food Microbiology 140, no. 2-3 (2010) : 277-281.
http://dx.doi.org/10.1016/j.ijfoodmicro.2010.04.013
---------- MLA ----------
Romero, S.M., Pinto, V.F., Patriarca, A., Vaamonde, G. "Ochratoxin A production by a mixed inoculum of Aspergillus carbonarius at different conditions of water activity and temperature" . International Journal of Food Microbiology, vol. 140, no. 2-3, 2010, pp. 277-281.
http://dx.doi.org/10.1016/j.ijfoodmicro.2010.04.013
---------- VANCOUVER ----------
Romero, S.M., Pinto, V.F., Patriarca, A., Vaamonde, G. Ochratoxin A production by a mixed inoculum of Aspergillus carbonarius at different conditions of water activity and temperature. Int. J. Food Microbiol. 2010;140(2-3):277-281.
http://dx.doi.org/10.1016/j.ijfoodmicro.2010.04.013