Artículo

Palomino, M.M.; Allievi, M.C.; Prado-Acosta, M.; Sanchez-Rivas, C.; Ruzal, S.M. "New method for electroporation of Lactobacillus species grown in high salt" (2010) Journal of Microbiological Methods. 83(2):164-167
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Abstract:

We here describe a new method for electroporation of Lactobacillus species, obligately homofermentative and facultatively heterofermentative, based on the cell-wall weakening resulting from growth in high-salt media. For L. casei, optimum transformation efficiency of up to 105 transformants per microgram of plasmid DNA was achieved following growth in the presence of 0.9M NaCl. Plasmids of different sizes and replication origins were also similarly transformed. These competent cells could be used either directly or stored frozen, up to 1month, for future use, with similar efficiency. This protocol was assayed with different Lactobacillus species: L. delbrueckii subsp. lactis, L. paracasei, L. plantarum and L. acidophilus, and it was found that they were transformed with similar efficiency. © 2010 Elsevier B.V.

Registro:

Documento: Artículo
Título:New method for electroporation of Lactobacillus species grown in high salt
Autor:Palomino, M.M.; Allievi, M.C.; Prado-Acosta, M.; Sanchez-Rivas, C.; Ruzal, S.M.
Filiación:Universidad de Buenos Aires, FCEyN, Dpto. de Química Biológica, Bs As, Argentina
Palabras clave:Electroporation; High salt growth; Lactobacillus; plasmid DNA; sodium chloride; article; bacterial cell wall; bacterial growth; bacterial strain; bacterium transformation; controlled study; cryopreservation; electroporation; fermentation; Lactobacillus; Lactobacillus acidophilus; Lactobacillus delbrueckii; Lactobacillus paracasei; Lactobacillus plantarum; nonhuman; priority journal; salt tolerance; Lactobacillus; Lactobacillus acidophilus; Lactobacillus casei; Lactobacillus delbrueckii subsp. lactis; Lactobacillus paracasei; Lactobacillus plantarum
Año:2010
Volumen:83
Número:2
Página de inicio:164
Página de fin:167
DOI: http://dx.doi.org/10.1016/j.mimet.2010.08.017
Título revista:Journal of Microbiological Methods
Título revista abreviado:J. Microbiol. Methods
ISSN:01677012
CODEN:JMIMD
CAS:sodium chloride, 7647-14-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01677012_v83_n2_p164_Palomino

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Citas:

---------- APA ----------
Palomino, M.M., Allievi, M.C., Prado-Acosta, M., Sanchez-Rivas, C. & Ruzal, S.M. (2010) . New method for electroporation of Lactobacillus species grown in high salt. Journal of Microbiological Methods, 83(2), 164-167.
http://dx.doi.org/10.1016/j.mimet.2010.08.017
---------- CHICAGO ----------
Palomino, M.M., Allievi, M.C., Prado-Acosta, M., Sanchez-Rivas, C., Ruzal, S.M. "New method for electroporation of Lactobacillus species grown in high salt" . Journal of Microbiological Methods 83, no. 2 (2010) : 164-167.
http://dx.doi.org/10.1016/j.mimet.2010.08.017
---------- MLA ----------
Palomino, M.M., Allievi, M.C., Prado-Acosta, M., Sanchez-Rivas, C., Ruzal, S.M. "New method for electroporation of Lactobacillus species grown in high salt" . Journal of Microbiological Methods, vol. 83, no. 2, 2010, pp. 164-167.
http://dx.doi.org/10.1016/j.mimet.2010.08.017
---------- VANCOUVER ----------
Palomino, M.M., Allievi, M.C., Prado-Acosta, M., Sanchez-Rivas, C., Ruzal, S.M. New method for electroporation of Lactobacillus species grown in high salt. J. Microbiol. Methods. 2010;83(2):164-167.
http://dx.doi.org/10.1016/j.mimet.2010.08.017