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Abstract:

Bromus auleticus (Trin.) is a grass native to the southern cone with important agronomical potential as fodder. Different breeding programs have been initiated with this grass, but plant tissue culture techniques could not be used because B. auleticus is recalcitrant. The aim of the present study was to develop a micropropagation protocol in the genus Bromus and to investigate if the association between B. auleticus and Epichloë endophytes affected in vitro culture and growth of micropropagated plantlets. In different micropropagation stages, better results were obtained with endophyte-infected (E+) seeds compared to endophyte-free (E−) seeds. The E+ seeds presented higher percentages of in vitro germination (82 ± 5 vs. 57 ± 6%), callus induction (72 ± 6 vs. 37 ± 6%), and plant regeneration from callus (89 ± 5 vs. 13 ± 5%). We also compared the biomass of shoot complexes and regenerated plantlets. After 4 weeks of culture, shoot complexes obtained from E+ seeds reached greater weight than the ones regenerated from E− seeds (173 ± 24 vs. 74 ± 9 mg). More than the 80% of the regenerated shoot complexes were rooted ex vitro and acclimated, regardless of their origin (E+ or E−). Finally, after 4 weeks of acclimatization, the plantlets regenerated from E+ seeds reached a greater weight than the ones from E− seeds, (461 ± 64 vs. 172 ± 25 mg). These results indicate that the use of endophyte-infected (E+) seeds enhances significantly B. auleticus micropropagation and promotes growth of the regenerated plantlets. © 2018, Springer Nature B.V.

Registro:

Documento: Artículo
Título:The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds
Autor:Regalado, J.J.; Berdion, V.; Vignale, M.V.; Novas, M.V.; Pitta-Alvarez, S.I.; Iannone, L.J.
Filiación:Laboratorio de Cultivo Experimental de Plantas y Microalgas, No. 68, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina
Laboratorio de Micología y Fitopatología, No. 69, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina
Instituto de Micología y Botánica (INMIBO), CONICET - Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Callus culture; Ex vitro rooting; Fungal endophytes; Native pastures; Organogenesis; Forestry; Plants (botany); Tissue culture; Callus cultures; Ex vitro rooting; Fungal endophytes; Native pastures; Organogenesis; Seed; Biomass; Callus; Forestry; Growth; Plants; Tissue Culture; Weight
Año:2018
Volumen:135
Número:2
Página de inicio:279
Página de fin:286
DOI: http://dx.doi.org/10.1007/s11240-018-1462-1
Título revista:Plant Cell, Tissue and Organ Culture
Título revista abreviado:Plant Cell Tissue Organ Cult.
ISSN:01676857
CODEN:PTCED
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v135_n2_p279_Regalado

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Citas:

---------- APA ----------
Regalado, J.J., Berdion, V., Vignale, M.V., Novas, M.V., Pitta-Alvarez, S.I. & Iannone, L.J. (2018) . The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds. Plant Cell, Tissue and Organ Culture, 135(2), 279-286.
http://dx.doi.org/10.1007/s11240-018-1462-1
---------- CHICAGO ----------
Regalado, J.J., Berdion, V., Vignale, M.V., Novas, M.V., Pitta-Alvarez, S.I., Iannone, L.J. "The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds" . Plant Cell, Tissue and Organ Culture 135, no. 2 (2018) : 279-286.
http://dx.doi.org/10.1007/s11240-018-1462-1
---------- MLA ----------
Regalado, J.J., Berdion, V., Vignale, M.V., Novas, M.V., Pitta-Alvarez, S.I., Iannone, L.J. "The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds" . Plant Cell, Tissue and Organ Culture, vol. 135, no. 2, 2018, pp. 279-286.
http://dx.doi.org/10.1007/s11240-018-1462-1
---------- VANCOUVER ----------
Regalado, J.J., Berdion, V., Vignale, M.V., Novas, M.V., Pitta-Alvarez, S.I., Iannone, L.J. The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds. Plant Cell Tissue Organ Cult. 2018;135(2):279-286.
http://dx.doi.org/10.1007/s11240-018-1462-1