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Abstract:

We describe an encapsulation–dehydration procedure with prefreezing steps for the cryopreservation of rhizome bud explants of Asparagus officinalis L. cv. Morado de Huétor. With this procedure, survival of Rhizome buds was at least 84 and 42% developed to complete plantlets at 8 weeks. Flow cytometry and EST-SSR molecular markers were used to assess genetic stability of the regenerated material. Effects of preculture time in a medium rich in sucrose and prefreezing treatments (0 °C or/and − 20 °C) on plant recovery were evaluated. Rhizome Buds of the “Morado de Huétor” landrace were incubated in preculture medium (MS + 0.3 M sucrose) for 48 h, encapsulated in alginate beads and desiccated until a water content of 35%, prefrozen for one hour at 0 °C plus one hour at − 20 °C, followed by cryopreservation in liquid nitrogen, and then were rewarmed and recovered in ARBM medium for 6 weeks and finally incubated in ARBM-0 for 4 weeks. Analyses of ploidy and molecular stability of plantlets recovered from cryopreserved rhizome buds of two selected genotypes showed no differences compared with the mother plants. Cryopreservation of RB explants of A. officinalis with this Encapsulation–Dehydration procedure will be useful in long-term preservation programs. © 2018, Springer Science+Business Media B.V., part of Springer Nature.

Registro:

Documento: Artículo
Título:Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability
Autor:Carmona-Martín, E.; Regalado, J.J.; Perán-Quesada, R.; Encina, C.L.
Filiación:Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, CSIC-UMA, Algarrobo-Costa, Málaga 29750, Spain
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Instituto de Micología y Botánica, UBA-CONICET, CABA, Buenos Aires, Argentina
Departamento de Biología Vegetal, Facultad de Ciencias, Campus de Teatinos s/n, Málaga, Spain
Palabras clave:Asparagus; Cryopreservation; Encapsulation–dehydration; Flow cytometry; Molecular markers; Rhizome bud; Dehydration; Flow cytometry; Recovery; Sugar (sucrose); Asparagus; Cryo-preservation; Dehydration procedure; Genetic stability; Long-term preservation; Molecular marker; Molecular stability; Rhizome bud; Biological materials preservation; Buds; Dehydration; Explants; Moisture Content; Peltogyne; Preservation; Recovery; Stability
Año:2018
Volumen:133
Número:3
Página de inicio:395
Página de fin:403
DOI: http://dx.doi.org/10.1007/s11240-018-1392-y
Título revista:Plant Cell, Tissue and Organ Culture
Título revista abreviado:Plant Cell Tissue Organ Cult.
ISSN:01676857
CODEN:PTCED
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v133_n3_p395_CarmonaMartin

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Citas:

---------- APA ----------
Carmona-Martín, E., Regalado, J.J., Perán-Quesada, R. & Encina, C.L. (2018) . Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability. Plant Cell, Tissue and Organ Culture, 133(3), 395-403.
http://dx.doi.org/10.1007/s11240-018-1392-y
---------- CHICAGO ----------
Carmona-Martín, E., Regalado, J.J., Perán-Quesada, R., Encina, C.L. "Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability" . Plant Cell, Tissue and Organ Culture 133, no. 3 (2018) : 395-403.
http://dx.doi.org/10.1007/s11240-018-1392-y
---------- MLA ----------
Carmona-Martín, E., Regalado, J.J., Perán-Quesada, R., Encina, C.L. "Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability" . Plant Cell, Tissue and Organ Culture, vol. 133, no. 3, 2018, pp. 395-403.
http://dx.doi.org/10.1007/s11240-018-1392-y
---------- VANCOUVER ----------
Carmona-Martín, E., Regalado, J.J., Perán-Quesada, R., Encina, C.L. Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability. Plant Cell Tissue Organ Cult. 2018;133(3):395-403.
http://dx.doi.org/10.1007/s11240-018-1392-y