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Regalado, J.J.; Carmona-Martin, E.; López-Granero, M.; Jiménez-Araujo, A.; Castro, P.; Encina, C.L. "Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk" (2018) Plant Cell, Tissue and Organ Culture. 132(3):573-578
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Abstract:

Asparagus macrorrhizus: is a new species, which has been recently described. It is limited to the area surrounding the “Mar Menor” lagoon, in Murcia (Spain), and is the only “Critically Endangered” species of the genus Asparagus. Despite being protected, the number of plants has decreased in the last years due to the urbanization of its natural habitat. This species is a valuable genetic resource for asparagus breeding because of its special characteristics. So, the development of a micropropagation protocol is crucial to its conservation and use in breeding programs. The micropropagation protocol from asparagus rhizome buds previously developed by our research group has been adapted for A. macrorrhizus. Rhizome buds of A. macrorrhizus were extracted, disinfected, and then cultured on Asparagus Rhizome Bud Medium (ARBM) consisting of MS medium supplemented with 0.3 mg l− 1 NAA, 0.1 mg l− 1 KIN, 2 mg l− 1 ancymidol and 6% sucrose. A percentage of 69.7 ± 8.0% of the rhizome buds developed shoots, but only 17.4 ± 7.9% of them rooted. To increase this low rooting rate, the shoots were cultured on Macrorrhizus Rooting Media (MRM) supplemented with three different concentrations of IBA. The highest rooting rate (55.0 ± 7.9%) was reached when shoots were incubated in MRM-2 consisting of MS medium supplemented with 2 mg l− 1 IBA and 4% sucrose. The acclimatization rate of the micropropagated plantlets was 90%. The method developed in this study allows the micropropagation of A. macrorrhizus, offering a new option to preserve this almost extinct species. © 2017, Springer Science+Business Media B.V., part of Springer Nature.

Registro:

Documento: Artículo
Título:Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk
Autor:Regalado, J.J.; Carmona-Martin, E.; López-Granero, M.; Jiménez-Araujo, A.; Castro, P.; Encina, C.L.
Filiación:Laboratorio de Cultivo de Tejidos y Biotecnología, Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, (CSIC-UMA), Algarrobo-Costa, Málaga 29750, Spain
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Laboratorio de Cultivo Experimental de Plantas y Microalgas, No. 68, Ciudad Autónoma de Buenos Aires, Argentina
CONICET - Universidad de Buenos Aires, Instituto de Micología y Botánica (INMIBO), Ciudad Autónoma de Buenos Aires, Argentina
Dpto. Genética, ETSIAM, Universidad de Córdoba, Campus de Rabanales, Edif. C-5, Córdoba, 14071, Spain
Dpto. Fitoquímica de Alimentos. Instituto de la Grasa (CSIC) – Universidad Pablo de Olavide, Edificio 46. Ctra. de Utrera, km. 1, Sevilla, 41013, Spain
Palabras clave:Asparagus spp; Endangered species; Flow cytometry; Germplasm preservation; Rhizome buds; Conservation; Flow cytometry; Sugar (sucrose); Asparagus spp; Critically endangered; Endangered species; Extinction risks; Genetic resources; Germplasms; Micropropagated plantlets; Rhizome buds; Plants (botany); Biodiversity; Genetic Engineering; Plants; Risk Assessment
Año:2018
Volumen:132
Número:3
Página de inicio:573
Página de fin:578
DOI: http://dx.doi.org/10.1007/s11240-017-1346-9
Título revista:Plant Cell, Tissue and Organ Culture
Título revista abreviado:Plant Cell Tissue Organ Cult.
ISSN:01676857
CODEN:PTCED
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v132_n3_p573_Regalado

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Citas:

---------- APA ----------
Regalado, J.J., Carmona-Martin, E., López-Granero, M., Jiménez-Araujo, A., Castro, P. & Encina, C.L. (2018) . Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk. Plant Cell, Tissue and Organ Culture, 132(3), 573-578.
http://dx.doi.org/10.1007/s11240-017-1346-9
---------- CHICAGO ----------
Regalado, J.J., Carmona-Martin, E., López-Granero, M., Jiménez-Araujo, A., Castro, P., Encina, C.L. "Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk" . Plant Cell, Tissue and Organ Culture 132, no. 3 (2018) : 573-578.
http://dx.doi.org/10.1007/s11240-017-1346-9
---------- MLA ----------
Regalado, J.J., Carmona-Martin, E., López-Granero, M., Jiménez-Araujo, A., Castro, P., Encina, C.L. "Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk" . Plant Cell, Tissue and Organ Culture, vol. 132, no. 3, 2018, pp. 573-578.
http://dx.doi.org/10.1007/s11240-017-1346-9
---------- VANCOUVER ----------
Regalado, J.J., Carmona-Martin, E., López-Granero, M., Jiménez-Araujo, A., Castro, P., Encina, C.L. Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk. Plant Cell Tissue Organ Cult. 2018;132(3):573-578.
http://dx.doi.org/10.1007/s11240-017-1346-9