Regalado, J.J.; Vignale, M.V.; Novas, M.V.; Pitta-Alvarez, S.I.; Iannone, L.J."Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum" (2017) Plant Cell, Tissue and Organ Culture. 130(1):37-46
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Italian ryegrass (Lolium multiflorum) is an annual grass considered as one of the most important temperate forage grasses in the world. However, it is recalcitrant to plant tissue culture techniques hindering its genetic manipulation. Epichloë occultans is an endophytic fungus associated with L. multiflorum. This symbiosis causes improvements in physiological and ecological traits of the host plants. The objective of this work was to study the effect of E. occultans on L. multiflorum micropropagation. We compared the response of endophyte-infected (E+) and endophyte-free (E−) seeds in different micropropagation stages. The E+ seeds were more successful than E− seeds in in vitro germination (83 ± 5 vs. 63 ± 6%), callus induction (78 ± 5 vs. 57 ± 6%), callus proliferation (average diameter of 21.5 ± 1.3 mm in two subcultures vs 17.3 ± 0.8 mm in three subcultures) and plant regeneration from callus (83 ± 7 vs. 30 ± 8%). These results indicate that E. occultans enhances significantly L. multiflorum micropropagation. The use of endophyte-infected (E+) seeds can be a solution to make this grass more amenable to different biotechnological tools, such as the genetic transformation. © 2017, Springer Science+Business Media Dordrecht.


Documento: Artículo
Título:Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum
Autor:Regalado, J.J.; Vignale, M.V.; Novas, M.V.; Pitta-Alvarez, S.I.; Iannone, L.J.
Filiación:Laboratorio de Cultivo Experimental de Plantas y Microalgas, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, No. 68, Ciudad Autónoma de Buenos Aires, Argentina
Laboratorio de Micología y Fitopatología, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, No. 69, Ciudad Autónoma de Buenos Aires, Argentina
Instituto de Micología y Botánica (INMIBO), Universidad de Buenos Aires-CONICET, Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Callus culture; Fungal endophytes; Grasses; Italian ryegrass; Organogenesis; Plant regeneration; Nucleic acids; Plants (botany); Tissue culture; Callus cultures; Fungal endophytes; Grasses; Italian ryegrass; Organogenesis; Plant regeneration; Seed; Efficiency; Fungi; Plants; Reforestation
Página de inicio:37
Página de fin:46
Título revista:Plant Cell, Tissue and Organ Culture
Título revista abreviado:Plant Cell Tissue Organ Cult.


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---------- APA ----------
Regalado, J.J., Vignale, M.V., Novas, M.V., Pitta-Alvarez, S.I. & Iannone, L.J. (2017) . Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum. Plant Cell, Tissue and Organ Culture, 130(1), 37-46.
---------- CHICAGO ----------
Regalado, J.J., Vignale, M.V., Novas, M.V., Pitta-Alvarez, S.I., Iannone, L.J. "Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum" . Plant Cell, Tissue and Organ Culture 130, no. 1 (2017) : 37-46.
---------- MLA ----------
Regalado, J.J., Vignale, M.V., Novas, M.V., Pitta-Alvarez, S.I., Iannone, L.J. "Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum" . Plant Cell, Tissue and Organ Culture, vol. 130, no. 1, 2017, pp. 37-46.
---------- VANCOUVER ----------
Regalado, J.J., Vignale, M.V., Novas, M.V., Pitta-Alvarez, S.I., Iannone, L.J. Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum. Plant Cell Tissue Organ Cult. 2017;130(1):37-46.