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Regalado, J.J.; Carmona-Martín, E.; Querol, V.; Veléz, C.G.; Encina, C.L.; Pitta-Alvarez, S.I. "Production of compact petunias through polyploidization" (2017) Plant Cell, Tissue and Organ Culture. 129(1):61-71
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Abstract:

Petunia, a commercially important ornamental plant worldwide, has been subjected to breeding programs that have yielded a high number of varieties. One of the key factors in the commercial value of these varieties is plant compactness. Currently, compact petunias are obtained through the application of expensive, harmful and short-lasting chemicals. To avoid the use of these chemicals, transgenic plants that over-express dwarf-inducing genes have been recently proposed as an alternative, but the current legislation regarding transgenic plants restricts their commercialization. In this work, we studied the effect of polyploidization in the plant architecture of Petunia axillaris, an Argentine native petunia. We developed a new polyploidization protocol that consisted in culturing petunia leaves in RL medium (MS medium supplemented with 30 g l−1 sucrose, 1 mg l−1 BA and 0.2 mg l−1 IAA) supplemented with 0.2 g l−1 colchicine for 15 days. This protocol allowed the regeneration of stable autotetraploid petunias (polyploidization rate: 29.0 ± 8,2%), which were 54% more compact than the diploid ones. Furthermore, they exhibited no variations in agronomical traits compared to the initial genotypes, except for a short delay in blooming. These autotetraploid plants can be used in different breeding programs and the polyploidization method developed can be tested in others cultivars of the genus Petunia for the same purpose. © 2017, Springer Science+Business Media Dordrecht.

Registro:

Documento: Artículo
Título:Production of compact petunias through polyploidization
Autor:Regalado, J.J.; Carmona-Martín, E.; Querol, V.; Veléz, C.G.; Encina, C.L.; Pitta-Alvarez, S.I.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Instituto de Micología y Botánica, UBA-CONICET, CABA, Piso 4, Pabellón II, Lab 68, Av. Int. Güiraldes 2620, Buenos Aires, C1428EHA, Argentina
Laboratorio de Cultivo de Tejidos y Biotecnología, Instituto de Horticultura Subtropical y Mediterránea La Mayora, CSIC-UMA, Algarrobo-Costa, Malaga 29750, Spain
Palabras clave:Autotetraploids; Colchicine; Organogenesis; Ornamentals; Petunia axillaris; Plant architecture; Plant compactness, dwarfing; Chromosomes; Autotetraploids; Colchicine; Organogenesis; Ornamentals; Petunia axillaris; Plant architecture; Plant compactness, dwarfing; Plants (botany); Breeding; Plants
Año:2017
Volumen:129
Número:1
Página de inicio:61
Página de fin:71
DOI: http://dx.doi.org/10.1007/s11240-016-1156-5
Título revista:Plant Cell, Tissue and Organ Culture
Título revista abreviado:Plant Cell Tissue Organ Cult.
ISSN:01676857
CODEN:PTCED
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v129_n1_p61_Regalado

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Citas:

---------- APA ----------
Regalado, J.J., Carmona-Martín, E., Querol, V., Veléz, C.G., Encina, C.L. & Pitta-Alvarez, S.I. (2017) . Production of compact petunias through polyploidization. Plant Cell, Tissue and Organ Culture, 129(1), 61-71.
http://dx.doi.org/10.1007/s11240-016-1156-5
---------- CHICAGO ----------
Regalado, J.J., Carmona-Martín, E., Querol, V., Veléz, C.G., Encina, C.L., Pitta-Alvarez, S.I. "Production of compact petunias through polyploidization" . Plant Cell, Tissue and Organ Culture 129, no. 1 (2017) : 61-71.
http://dx.doi.org/10.1007/s11240-016-1156-5
---------- MLA ----------
Regalado, J.J., Carmona-Martín, E., Querol, V., Veléz, C.G., Encina, C.L., Pitta-Alvarez, S.I. "Production of compact petunias through polyploidization" . Plant Cell, Tissue and Organ Culture, vol. 129, no. 1, 2017, pp. 61-71.
http://dx.doi.org/10.1007/s11240-016-1156-5
---------- VANCOUVER ----------
Regalado, J.J., Carmona-Martín, E., Querol, V., Veléz, C.G., Encina, C.L., Pitta-Alvarez, S.I. Production of compact petunias through polyploidization. Plant Cell Tissue Organ Cult. 2017;129(1):61-71.
http://dx.doi.org/10.1007/s11240-016-1156-5