Artículo

Chasseing, N.A.; Trejo, Y.G.; Bordenave, R.H.; Bullorsky, E.O.; Diaz, N.B.; Rumi, L.S. "Bone marrow fibroblastic progenitors in patients with advanced breast cancer" (1997) Breast Cancer Research and Treatment. 45(3):211-218
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Abstract:

Bone marrow fibroblast colony-forming cells (CFU-F) were studied in fifteen consecutive untreated breast cancer patients (BCP) with clinical stages III and IV, and in sixteen normal controls (NC). A decreased number of CFU-F was observed in BCP compared to NC (p < 0.004). Confluence of the adherent cell layer was observed in all normal bone marrow mononuclear cells (MC) cultures, while a lower proportion of cultures from BCP (11/15) showed confluent adherent cell layers. When MC cultures of BCP were treated with indomethacin (Indo, 10 -6 M) 50% of them increased the number of CFU-F compared to the value obtained without treatment. In addition, a significant increase in the release of PGE2 in BCP cultures was observed before Indo treatment. Moreover, after MC were fractionated into adherent and non-adherent progenitors, the CFU-F decreased in both types of fractions of BCP compared to NC value (p < 0.02 and < 0.05, respectively). The number of light density MC per 10 ml of bone marrow aspirate and the number of trypsin-sensitive adherent progenitors were lower than NC in BCP (p < 0.02 and 0.013, respectively). Total MC and fibroblasts (fourth passage) were cultivated to evaluate the production of interleukin-1β (IL-1β) by ELISA methodology. Results indicated no difference of IL-1β spontaneous release when total MC cultures of both groups were compared. However, the levels of this cytokine were lower (< 10 pg/ml) in fibroblast culture supernatants of BCP compared to NC (1217 ± 74 pg/ml). Fibroblast cultures from BCP showed low or no release of IL-1β after muramyl-dipeptide (MDP, 1 μg/ml) stimulation. In conclusion, the defective proliferative and confluence capacity of BCP fibroblastic progenitors may be related to the decrease in the production of IL-1β by these precursors.

Registro:

Documento: Artículo
Título:Bone marrow fibroblastic progenitors in patients with advanced breast cancer
Autor:Chasseing, N.A.; Trejo, Y.G.; Bordenave, R.H.; Bullorsky, E.O.; Diaz, N.B.; Rumi, L.S.
Filiación:Consejo Nac. de Invest. Cie. y T., Argentina
Inst. de Biol. Medicina Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Deparment of Oncology, Hospital Zonal de Agudos I. Iriarte, Quilmes, Pcia. de Buenos Aires, Argentina
Department of Hematology, Hospital Británico, Buenos Aires, Argentina
Hematology Division, Department of Clinical Biochemistry, Universidad de Buenos Aires, Argentina
Inst. de Biol. y Med. Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Fibroblasts and interleukin-β; indometacin; interleukin 1beta; muramyl dipeptide; prostaglandin E2; trypsin; adherent cell; advanced cancer; article; bone marrow biopsy; bone marrow cell; breast cancer; cancer staging; cell culture; cell stimulation; clinical article; colony forming cell; controlled study; enzyme linked immunosorbent assay; female; fibroblast; fibroblast culture; human; human cell; mononuclear cell; priority journal; prostaglandin release; stem cell; supernatant; Biopsy, Needle; Bone Marrow Cells; Breast; Breast Neoplasms; Cells, Cultured; Dinoprostone; Female; Fibroblasts; Humans; Interleukin-1; Stem Cells
Año:1997
Volumen:45
Número:3
Página de inicio:211
Página de fin:218
DOI: http://dx.doi.org/10.1023/A:1005757218114
Título revista:Breast Cancer Research and Treatment
Título revista abreviado:BREAST CANCER RES. TREAT.
ISSN:01676806
CODEN:BCTRD
CAS:indometacin, 53-86-1, 74252-25-8, 7681-54-1; prostaglandin E2, 363-24-6; trypsin, 9002-07-7; Dinoprostone, 363-24-6; Interleukin-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676806_v45_n3_p211_Chasseing

Referencias:

  • Tavassoli, M., Hardy, C.L., Molecular basis of homing of intravenously transplanted stem cells to the marrow (1990) Blood, 76, pp. 1059-1070
  • Mayani, H., Guilbert, L.J., Janowska-Wieczorek, A., Biology of hemopoietic microenvironment (1992) Eur J Haematol, 49, pp. 225-233
  • Andreoni, C., Moreau, I., Rigal, D., Long-term culture of human bone marrow (1990) I. Characterization of Adherent Cells in Flow Cytometry. Exp Hematol, 18, pp. 431-437
  • Bentley, S.A., The role and composition of the adherent layer in long-term bone marrow culture (1984) Long-term Bone Marrow Culture, pp. 141-156. , Wright DG, Greenberger J (eds) Alan R Liss Inc, New York
  • Greenberger, J.S., The hemopoietic microenvironment (1991) Crit Rev Oncol Hematol, 11, pp. 65-84
  • Castro Malaspina, H., Ebbel, W., Wang, S.Y., Human bone marrow fibroblast colony forming units (CFU-F) (1984) Myelofibrosis and the Biology of Connective Tissue, pp. 209-236. , Berk PD, Castro Malaspina H, Wasserman LR (eds) Alan R Liss Inc, New York
  • Friedenstein, A.J., Chailakhyan, R.K., Latsinik, N.V., Panasyuk, A.F., Keiliss-Borok, I.V., Stromal cells responsible for transferring the microenvironment of the hematopoietic tissue (1974) Transplantation, 17, pp. 331-340
  • Castro Malaspina, H., Gay, R.E., Resnick, G., Kapoor, N., Meyers, P., Chiarieri, D., McKenzie, S., Moore, M.A.S., Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny (1980) Blood, 56, pp. 289-301
  • Croizat, H., Eskinazi, D., Axelrad, A.A., Stromal cell colonies produced by non-adherent cells from long term human bone marrow cultures enhance erythropoietic burst formation (1986) Exp Hematol, 14, pp. 324-326
  • Clarke, E., McCann, S.R., Stromal colonies can be grown from the non-adherent cells in human long term bone marrow cultures (1991) Eur J Haematol, 46, pp. 296-300
  • Tsai, S., Patel, V., Beaumont, E., Lodish, H.F., Nathan, D.G., Sieff, C.A., Differential binding of erythroid and myeloid progenitors to fibroblasts and fibronectin (1987) Blood, 69, pp. 1587-1594
  • Moreau, I., Andreoni, C., Caux, C., Saeland, S., Rigal, D., Modification of human long-term bone marrow cultures: Establishment of a functional stromal microenvironment devoid of myeloid progenitors (1992) Eur J Haematol, 49, pp. 29-35
  • Kovacs, E.J., Fibrogenic cytokines: The role of immune mediators in the development of scar tissue (1991) Immunol Today, 12, pp. 17-23
  • Ershler, W.B., Ross, J., Finlay, J.L., Shahidi, N.T., Bone marrow microenvironment defect in congenital hypoplastic anemia (1980) N Engl J Med, 302, pp. 1321-1327
  • Hirala, J., Katsuno, M., Kaneko, S., Clinical significance of human bone marrow stromal cell colonies in acute leukemias (1986) Leuk Res, 12, pp. 1441-1445
  • Takahashi, M., Keating, A., Singer, J.W., A functional defect in irradiated adherent layers from chronic myelogenous leukemia long-term marrow cultures (1985) Exp Hematol, 13, pp. 926-931
  • Coutinho, L.H., Geary, C.G., Chang, J., Harrison, C., Testa, N.G., Functional studies of bone marrow haematopoietic and stroma cells in the myelodysplastic syndrome (MDS) (1990) British J Haematol, 75, pp. 16-25
  • Testa, N.G., Hendry, J.H., Molineux, G., Long term hone marrow damage in experimental systems and in patients after radiation or chemotherapy (1985) Anti Cancer Res, 5, p. 101
  • Knospe, W.H., Long term bone marrow damage after irradiation (1988) Hematopoiesis: Long Term Effects of Chemotherapy and Radiation, pp. 93-130. , Testa NG, Gale RP (eds) Marcel Dekker Inc
  • Pike, B.L., Robinson, W.A., Human bone marrow colony growth in agar-gel (1970) J Cell Physiol, 76, pp. 77-84
  • Welte, K., Platzer, E., Lu, L., Gabrilove, J., Levi, E., Mertelsman, R., Moore, M.A.S., Purification and biochemical characterization of human pluripotent hematopoietic colony stimulating factor (1985) Proc Natl Acad Sci USA, 82, pp. 1526-1530
  • Eaves, A.C., Eaves, C.J., Maintenance and proliferation control of primitive hemopoietic progenitors in long-term cultures of marrow cells (1988) Blood Cells, 14, pp. 355-368
  • Juneja, H.S., Gardner, F.H., Minguell, J.J., Helmer, R.E., Abnormal marrow fibroblasts in aplastic anemia (1984) Exp Hematol, 12, pp. 221-230
  • Korn, J.H., Halushka, P.V., Leroy, E.C., Mononuclear cell modulation of connective tissue function. Suppression of fibroblast growth by stimulation of endogenous prostaglandin production (1980) J Clin Invest, 65, pp. 543-554
  • Freundlich, B., Bomalaski, J.S., Neilson, E., Jimenez, S.A., Regulation of fibroblast proliferation and collagen synthesis by cytokines (1986) Immunol Today, 7, pp. 303-307
  • Paulsson, Y., Hammacher, A., Heldin, C., Westermark, B., Possible autocrine feed-back in the prereplicative phase of human fibroblasts (1987) Nature, 328, pp. 715-717
  • Lin, J.X., Vilcek, J., Tumor necrosis factor and interleukin-1 cause a rapid and transient stimulation of c-fos and c-myc mRNA levels in human fibroblasts (1987) J Biol Chem, 262, pp. 11908-11911

Citas:

---------- APA ----------
Chasseing, N.A., Trejo, Y.G., Bordenave, R.H., Bullorsky, E.O., Diaz, N.B. & Rumi, L.S. (1997) . Bone marrow fibroblastic progenitors in patients with advanced breast cancer. Breast Cancer Research and Treatment, 45(3), 211-218.
http://dx.doi.org/10.1023/A:1005757218114
---------- CHICAGO ----------
Chasseing, N.A., Trejo, Y.G., Bordenave, R.H., Bullorsky, E.O., Diaz, N.B., Rumi, L.S. "Bone marrow fibroblastic progenitors in patients with advanced breast cancer" . Breast Cancer Research and Treatment 45, no. 3 (1997) : 211-218.
http://dx.doi.org/10.1023/A:1005757218114
---------- MLA ----------
Chasseing, N.A., Trejo, Y.G., Bordenave, R.H., Bullorsky, E.O., Diaz, N.B., Rumi, L.S. "Bone marrow fibroblastic progenitors in patients with advanced breast cancer" . Breast Cancer Research and Treatment, vol. 45, no. 3, 1997, pp. 211-218.
http://dx.doi.org/10.1023/A:1005757218114
---------- VANCOUVER ----------
Chasseing, N.A., Trejo, Y.G., Bordenave, R.H., Bullorsky, E.O., Diaz, N.B., Rumi, L.S. Bone marrow fibroblastic progenitors in patients with advanced breast cancer. BREAST CANCER RES. TREAT. 1997;45(3):211-218.
http://dx.doi.org/10.1023/A:1005757218114