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Abstract:

We have determined the presence of transforming growth factor-β (TGF-β)-like polypeptides in mammary adenocarcinomas induced by medroxyprogesterone acetate (MPA) in BALB/c mice. In hormone-dependent tumors (HD) from nontreated and MPA-treated mice a high molecular weight (43 kDa) transforming activity was purified by Bio-Gel P-60 chromatography. This TGF was able to confer the neoplastic phenotype on NRK-49F cells without the addition of epidermal growth factor (EGF), though its activity was potentiated by EGF. It did not compete for binding to the EGF receptor, had no mitogenic activity on monolayer cultures of NRK fibroblasts, and was a potent inhibitor of DNA synthesis induced in these cells by EGF and insulin. In HD and hormone-independent tumors (HI) another TGF with a Mr of 13 kDa was isolated. This transforming activity showed the same biological properties as 43 kDa TGF, with the exception that in the absence of EGF it did not stimulate soft agar growth of NRK-49F cells. The synthesis of both factors in 'in vivo' HD tumors seems to be under MPA control, since it is much lower in HD tumors from MPA-treated mice. Further purification of the 13 and 43 kDa TGFs by hydrophobic interaction HPLC demonstrated that each one eluted in a different position, and that their elution profile differed from the TGF-β from human platelets. The biological activity of the 13 and 43 kDa TGFs was not neutralized by a specific anti-TGF-β antibody. © 1990 Kluwer Academic Publishers.

Registro:

Documento: Artículo
Título:Transforming growth factor-β activities in 'in vivo' lines of hormone-dependent and independent mammary adenocarcinomas induced by medroxyprogesterone acetate in BALB/c mice
Autor:Elizalde, P.V.; Lanari, C.; Kordon, E.; Tezón, J.; Charreau, E.H.
Filiación:Instituto de Biología y Medicina Experimental, Obligado 2490, Buenos Aires, 1428, Argentina
Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, J.A. Pacheco de Melo 3081, Buenos Aires, 1425, Argentina
Departamento de Química Biológica, FCEN., Universidad de Buenos Aires, Argentina
Palabras clave:inhibitory growth control; mammary adenocarcinomas; medroxyprogesterone acetate; transforming growth factor-β; medroxyprogesterone acetate; transforming growth factor beta; animal experiment; article; breast adenocarcinoma; female; mouse; nonhuman; subcutaneous drug administration; Adenocarcinoma; Animal; Cell Division; Cell Transformation, Neoplastic; Chromatography, High Pressure Liquid; DNA Replication; Drug Interactions; Epidermal Growth Factor; Fibroblasts; Gene Expression Regulation, Neoplastic; Insulin; Mammary Neoplasms, Experimental; Medroxyprogesterone; Medroxyprogesterone 17-Acetate; Mice; Mice, Inbred BALB C; Neoplasm Proteins; Neoplasms, Hormone-Dependent; Rats; Support, Non-U.S. Gov't; Transforming Growth Factor beta
Año:1990
Volumen:16
Número:1
Página de inicio:29
Página de fin:39
DOI: http://dx.doi.org/10.1007/BF01806573
Título revista:Breast Cancer Research and Treatment
Título revista abreviado:Breast Cancer Res Tr
ISSN:01676806
CODEN:BCTRD
CAS:Epidermal Growth Factor, 62229-50-9; Insulin, 11061-68-0; Medroxyprogesterone 17-Acetate, 71-58-9; Medroxyprogesterone, 520-85-4; Neoplasm Proteins; Transforming Growth Factor beta
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676806_v16_n1_p29_Elizalde

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Citas:

---------- APA ----------
Elizalde, P.V., Lanari, C., Kordon, E., Tezón, J. & Charreau, E.H. (1990) . Transforming growth factor-β activities in 'in vivo' lines of hormone-dependent and independent mammary adenocarcinomas induced by medroxyprogesterone acetate in BALB/c mice. Breast Cancer Research and Treatment, 16(1), 29-39.
http://dx.doi.org/10.1007/BF01806573
---------- CHICAGO ----------
Elizalde, P.V., Lanari, C., Kordon, E., Tezón, J., Charreau, E.H. "Transforming growth factor-β activities in 'in vivo' lines of hormone-dependent and independent mammary adenocarcinomas induced by medroxyprogesterone acetate in BALB/c mice" . Breast Cancer Research and Treatment 16, no. 1 (1990) : 29-39.
http://dx.doi.org/10.1007/BF01806573
---------- MLA ----------
Elizalde, P.V., Lanari, C., Kordon, E., Tezón, J., Charreau, E.H. "Transforming growth factor-β activities in 'in vivo' lines of hormone-dependent and independent mammary adenocarcinomas induced by medroxyprogesterone acetate in BALB/c mice" . Breast Cancer Research and Treatment, vol. 16, no. 1, 1990, pp. 29-39.
http://dx.doi.org/10.1007/BF01806573
---------- VANCOUVER ----------
Elizalde, P.V., Lanari, C., Kordon, E., Tezón, J., Charreau, E.H. Transforming growth factor-β activities in 'in vivo' lines of hormone-dependent and independent mammary adenocarcinomas induced by medroxyprogesterone acetate in BALB/c mice. Breast Cancer Res Tr. 1990;16(1):29-39.
http://dx.doi.org/10.1007/BF01806573