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Abstract:

Here, we present a new toxicity bioassay (CO2-TOX), able to detect toxic or inhibitory compounds in water samples, based on the quantification of Pseudomonas putida KT2440 CO2 production. The metabolically produced CO2 was measured continuously and directly in the liquid assay media, with a potentiometric gas electrode. The optimization studies were performed using as a model toxicant 3,5-DCP (3,5-dichlorophenol); later, heavy metals (Pb2+, Cu2+, or Zn2+) and a metalloid (As5+) were assayed. The response to toxics was evident after 15 min of incubation and at relatively low concentrations (e.g., 1.1 mg/L of 3,5-DCP), showing that the CO2-TOX bioassay is fast and sensitive. The EC50 values obtained were 4.93, 0.12, 6.05, 32.17, and 37.81 mg/L for 3,5-DCP, Cu2+, Zn2+, As5+, and Pb2+, respectively, at neutral pH. Additionally, the effect of the pH of the sample and the use of lyophilized bacteria were also analyzed showing that the bioassay can be implemented in different conditions. Moreover, highly turbid samples and samples with very low oxygen levels were measured successfully with the new instrumental bioassay described here. Finally, simulated samples containing 3,5-DCP or a heavy metal mixture were tested using the proposed bioassay and a standard ISO bioassay, showing that our test is more sensible to the phenol but less sensible to the metal mixtures. Therefore, we propose CO2-TOX as a rapid, sensitive, low-cost, and robust instrumental bioassay that could perform as an industrial wastewater-process monitor among other applications. © 2015, Springer International Publishing Switzerland.

Registro:

Documento: Artículo
Título:A new P. putida instrumental toxicity bioassay
Autor:Figueredo, F.; Abrevaya, X.C.; Cortón, E.
Filiación:Laboratory of Biosensors and Bioanalysis (LABB), Departamento de Química Biológica e IQUIBICEN-CONICET, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, 1428, Argentina
Instituto de Astronomía y Física del Espacio, CONICET–UBA, Pabellón IAFE, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, 1428, Argentina
Palabras clave:Acute toxicity; CO2 potentiometric electrode; Lyophilized bacteria; Respirometry; Bacteria; Copper; Electrodes; Health hazards; Heavy metals; Lead; Mixtures; Potentiometers (electric measuring instruments); Toxicity; Zinc; 3 ,5-dichlorophenol; Acute toxicity; Industrial wastewaters; Inhibitory compounds; Low concentrations; Optimization studies; Potentiometric electrode; Respirometry; Bioassay; 3,5 dichlorophenol; arsenic; carbon dioxide; copper ion; lead; oxygen; surface water; water; zinc ion; 3,5-dichlorophenol; chlorophenol; dangerous goods; phenol; phenol derivative; waste water; water pollutant; bioassay; detection method; electrode; heavy metal; inhibitor; metabolism; microbial activity; optimization; quantitative analysis; toxicity; Article; breathing rate; carbon dioxide breathing; carbon dioxide toxicity bioassay; chemical binding; concentration response; controlled study; cost effectiveness analysis; EC50; incubation time; lyophilisate; metal mixture; nonhuman; pH; process optimization; Pseudomonas cell multiplication inhibition test; Pseudomonas putida; sensitivity analysis; standardization; survival rate; toxicity testing; turbidity; waste water management; bioassay; dangerous goods; drug effects; electrode; environmental monitoring; industry; potentiometry; procedures; Pseudomonas putida; toxicity; toxicity testing; waste water; water pollutant; Pseudomonas putida; Biological Assay; Chlorophenols; Electrodes; Environmental Monitoring; Hazardous Substances; Industry; Oxygen; Phenol; Phenols; Potentiometry; Pseudomonas putida; Toxicity Tests; Waste Water; Water Pollutants, Chemical
Año:2015
Volumen:187
Número:5
DOI: http://dx.doi.org/10.1007/s10661-015-4499-1
Título revista:Environmental Monitoring and Assessment
Título revista abreviado:Environ. Monit. Assess.
ISSN:01676369
CODEN:EMASD
CAS:3,5 dichlorophenol, 591-35-5; arsenic, 7440-38-2; carbon dioxide, 124-38-9, 58561-67-4; lead, 7439-92-1, 13966-28-4; oxygen, 7782-44-7; water, 7732-18-5; zinc ion, 23713-49-7; chlorophenol, 25167-80-0; phenol, 108-95-2, 3229-70-7; 3,5-dichlorophenol; Chlorophenols; Hazardous Substances; Oxygen; Phenol; Phenols; Waste Water; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676369_v187_n5_p_Figueredo

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Citas:

---------- APA ----------
Figueredo, F., Abrevaya, X.C. & Cortón, E. (2015) . A new P. putida instrumental toxicity bioassay. Environmental Monitoring and Assessment, 187(5).
http://dx.doi.org/10.1007/s10661-015-4499-1
---------- CHICAGO ----------
Figueredo, F., Abrevaya, X.C., Cortón, E. "A new P. putida instrumental toxicity bioassay" . Environmental Monitoring and Assessment 187, no. 5 (2015).
http://dx.doi.org/10.1007/s10661-015-4499-1
---------- MLA ----------
Figueredo, F., Abrevaya, X.C., Cortón, E. "A new P. putida instrumental toxicity bioassay" . Environmental Monitoring and Assessment, vol. 187, no. 5, 2015.
http://dx.doi.org/10.1007/s10661-015-4499-1
---------- VANCOUVER ----------
Figueredo, F., Abrevaya, X.C., Cortón, E. A new P. putida instrumental toxicity bioassay. Environ. Monit. Assess. 2015;187(5).
http://dx.doi.org/10.1007/s10661-015-4499-1