Abstract:
Museum specimens are often the only source of genetic material for many species of cetaceans. Most of the protocols that have been developed to extract DNA from teeth and bone samples involve mechanical or chemical disintegration of the material. An alternative method to the mechanical reduction process is presented here for 212 Commerson's dolphin (Cephalorhynchus commersonii) samples collected in Tierra del Fuego, Argentina, from 1974 to 2004. From the total samples, 82.5% (n = 175) allow for amplification to occur of a minimum length of 200 pb for the mtDNA control region, 66.5% (n = 141) of 400 bp, and 51% (n = 108) of 500 bp. A total of 11 haplotypes were defined from 10 polymorphic sites. From the initial number of samples, 64.6% could be sexed via molecular markers. This method is effective for processing large quantities of degraded samples over a short time period, analysing a representative number of mtDNA haplotypes, and allowing robust estimation of historical genetic diversity and trends over time.
Registro:
Documento: |
Artículo
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Título: | Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina |
Autor: | Pimper, L.E.; Remis, M.I.; Goodall, R.N.P.; Baker, C.S. |
Filiación: | Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Buenos Aires, Argentina Museo Acatushún de Aves y Mamíferos Marinos Australes (AMMA), Sarmiento 44, 9410 Ushuaia, Tierra del Fuego, Argentina Centro Austral de Investigaciones Científicas (CADIC), Bernardo Houssay 200, 9410 Ushuaia, Tierra del Fuego, Argentina School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand Marine Mammal Institute, Hatfield Marine Science Center, Oregon State University, 2030 SE Marine Science Drive, Newport, OR 97365, United States
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Palabras clave: | Bones; Cephalorhynchus commersonii; Commerson's dolphin; DNA extraction; Population genetic analysis; Teeth; biodegradation; biomarker; bone; DNA; dolphin; genetic analysis; population genetics; sex determination; tooth; Argentina; South America; Tierra del Fuego [(PRV) Argentina]; Cephalorhynchus commersonii; Cetacea |
Año: | 2009
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Volumen: | 35
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Número: | 3
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Página de inicio: | 330
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Página de fin: | 333
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DOI: |
http://dx.doi.org/10.1578/AM.35.3.2009.330 |
Título revista: | Aquatic Mammals
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Título revista abreviado: | Aquatic Mamm.
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ISSN: | 01675427
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01675427_v35_n3_p330_Pimper |
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Citas:
---------- APA ----------
Pimper, L.E., Remis, M.I., Goodall, R.N.P. & Baker, C.S.
(2009)
. Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina. Aquatic Mammals, 35(3), 330-333.
http://dx.doi.org/10.1578/AM.35.3.2009.330---------- CHICAGO ----------
Pimper, L.E., Remis, M.I., Goodall, R.N.P., Baker, C.S.
"Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina"
. Aquatic Mammals 35, no. 3
(2009) : 330-333.
http://dx.doi.org/10.1578/AM.35.3.2009.330---------- MLA ----------
Pimper, L.E., Remis, M.I., Goodall, R.N.P., Baker, C.S.
"Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina"
. Aquatic Mammals, vol. 35, no. 3, 2009, pp. 330-333.
http://dx.doi.org/10.1578/AM.35.3.2009.330---------- VANCOUVER ----------
Pimper, L.E., Remis, M.I., Goodall, R.N.P., Baker, C.S. Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina. Aquatic Mamm. 2009;35(3):330-333.
http://dx.doi.org/10.1578/AM.35.3.2009.330