Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Museum specimens are often the only source of genetic material for many species of cetaceans. Most of the protocols that have been developed to extract DNA from teeth and bone samples involve mechanical or chemical disintegration of the material. An alternative method to the mechanical reduction process is presented here for 212 Commerson's dolphin (Cephalorhynchus commersonii) samples collected in Tierra del Fuego, Argentina, from 1974 to 2004. From the total samples, 82.5% (n = 175) allow for amplification to occur of a minimum length of 200 pb for the mtDNA control region, 66.5% (n = 141) of 400 bp, and 51% (n = 108) of 500 bp. A total of 11 haplotypes were defined from 10 polymorphic sites. From the initial number of samples, 64.6% could be sexed via molecular markers. This method is effective for processing large quantities of degraded samples over a short time period, analysing a representative number of mtDNA haplotypes, and allowing robust estimation of historical genetic diversity and trends over time.

Registro:

Documento: Artículo
Título:Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina
Autor:Pimper, L.E.; Remis, M.I.; Goodall, R.N.P.; Baker, C.S.
Filiación:Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Buenos Aires, Argentina
Museo Acatushún de Aves y Mamíferos Marinos Australes (AMMA), Sarmiento 44, 9410 Ushuaia, Tierra del Fuego, Argentina
Centro Austral de Investigaciones Científicas (CADIC), Bernardo Houssay 200, 9410 Ushuaia, Tierra del Fuego, Argentina
School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand
Marine Mammal Institute, Hatfield Marine Science Center, Oregon State University, 2030 SE Marine Science Drive, Newport, OR 97365, United States
Palabras clave:Bones; Cephalorhynchus commersonii; Commerson's dolphin; DNA extraction; Population genetic analysis; Teeth; biodegradation; biomarker; bone; DNA; dolphin; genetic analysis; population genetics; sex determination; tooth; Argentina; South America; Tierra del Fuego [(PRV) Argentina]; Cephalorhynchus commersonii; Cetacea
Año:2009
Volumen:35
Número:3
Página de inicio:330
Página de fin:333
DOI: http://dx.doi.org/10.1578/AM.35.3.2009.330
Título revista:Aquatic Mammals
Título revista abreviado:Aquatic Mamm.
ISSN:01675427
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01675427_v35_n3_p330_Pimper

Referencias:

  • Baker, C.S., Flórez-González, L., Abernethy, R.B., Rosenbaum, H.C., Slade, R.W., Capella, J., Mitochondrial DNA variation and maternal gene flow among humpback whales of the southern hemisphere (1998) Marine Mammal Science, 14, pp. 721-737
  • Boom, R., Sol, C.J.A., Cansen, C.L., Wertheim-van Pillen, P.M.E., van der Noorda, J., Rapid and simple method for purification of nucleic acids (1990) Journal of Clinical Microbiology, 28, pp. 495-503
  • Dalebout, M.L., van Helden, A., Van Waerebeek, K., Baker, C.S., Molecular genetic identification of southern hemisphere beaked whales (Cetacea: Ziphiidae) (1998) Molecular Ecology, 7, pp. 687-694
  • Dalebout, M.L., Baker, C.S., Cockroft, V.G., Mead, J.G., Yamada, T.K., A comprehensive molecular taxonomy of beaked whales (Cetacea: Ziphiidae) using a validated mitochondrial and nuclear DNA database (2004) Journal of Heredity, 95, pp. 459-473
  • Excoffier, L, Laval, G, & Schneider, S, 2005, Arlequin version 3.0: An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online, 1, 47-50; Gilson, A., Syvanen, M., Levine, K., Banks, J., Deer gender determination by polymerase chain reaction: Validation study and application to tissues, bloodstains, and hair forensic samples from California (1998) California Fish and Game, 84, pp. 159-169
  • Goodall, R.N.P., Cameron, I.S., Exploitation of small cetaceans off southern South America (1980) Report of the International Whaling Commission, pp. 445-450. , 30
  • Goodall, R. N. P., Schiavini, A. C. M., & Fermani, C. (1994). Net fisheries and net mortality of small cetaceans off Tierra del Fuego, Argentina. In W. F. Perrin, G. P. Donovan, & J. Barlow (Eds.), Gillnets and cetaceans. Report of the International Whaling Commission (Special Issue 15), 295-306; Goodall, R. N. P., Galeazzi, A. R., Leatherwood, S., Miller, K. W., Cameron, I. S., Kastelein, R. K., et al. (1988). Studies of Commerson's dolphins, Cephalorhynchus commersonii, off Tierra del Fuego, 1976-1984, with a review of information on the species in the South Atlantic. In R. L. Brownell, Jr. & G. P. Donovan (Eds.), The biology of the genus Cephalorhynchus. Report of the International Whaling Commission (Special Issue 9), 3-70; Hall, T.A., BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT (1999) Nucleic Acids Symposium Series, pp. 95-98
  • Hanaoka, Y., Inoue, M., Tsai, T.H., Minaguchi, K., Fundamental and practical study for DNA analysis using tooth as a source of DNA (1995) Nippon Hoigaku Zasshi, 49, pp. 1-10
  • Higgins, D.G., Sharp, P.M., Clustal: A package for performing multiple sequence alignment on a microcomputer (1988) Gene, 73, pp. 237-244
  • Höss, M., Pääbo, S., DNA purification from Pleistocene bones by a silica-based purification method (1993) Nucleic Acids Research, 21, pp. 3913-3914
  • Kemp, B.M., Smith, D.G., Use of bleach to eliminate contaminating DNA from the surface of bones and teeth (2005) Forensic Science International, 154, pp. 53-61
  • Lacépède, B.G.E., (1804) Histoire naturelle des cétacés, , Paris: Chez Plasson
  • Maddison, W. P, & Maddison, D. R, 1992, MacClade Release Version 3.04, Analysis of phylogeny and character evolution. Sunderland, MA: Sinauer Associates; Nei, M., (1987) Molecular evolutionary genetics, , New York: Columbia University Press
  • Pichler, F.B., Baker, C.S., Loss of genetic diversity in the endemic Hector's dolphin due to fisheries-related mortality (2000) Proceedings of the Royal Society of London, Series B, 267, pp. 97-102
  • Pichler, F.B., Olavarría, C., Resolving Chilean dolphin (Cephalorhynchus eutropia, Gray 1846) synonymy by sequencing DNA extracted from teeth of museum specimens (2001) Revista de Biología Marina y Oceanografía, 36 (1), pp. 117-121
  • Pichler, F.B., Dalebout, M.L., Baker, C.S., Non-destructive DNA extraction from sperm whale teeth and scrimshaw (2001) Molecular Ecology Notes, 1, pp. 106-109
  • Pichler, F.B., Robineau, D., Goodall, R.N.P., Meÿer, M.A., Olivarría, C., Baker, C.S., Origin and radiation of Southern Hemisphere coastal dolphins (genus Cephalorhynchus) (2001) Molecular Ecology, 10, pp. 2215-2223
  • Rohland, N., Siedel, H., Hofreiter, M., Nondestructive DNA extraction method for mitochondrial DNA analyses of museum specimens (2004) BioTechniques, 36, pp. 814-821
  • Rosenbaum, H.C., Egan, M.G., Clapham, P.J., Brownell Jr., R.L., Desalle, R., An effective method for isolating DNA from historical specimens of baleen (1997) Molecular Ecology, 6, pp. 677-681
  • Rosenbaum, H.C., Egan, M.G., Clapham, P.J., Brownell Jr., R.L., Malik, S., Brown, M.W., Utility of North Atlantic Right Whale museum specimens for assessing changes in genetic diversity (1837) Conservation Biology, 14 (6). , 2000

Citas:

---------- APA ----------
Pimper, L.E., Remis, M.I., Goodall, R.N.P. & Baker, C.S. (2009) . Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina. Aquatic Mammals, 35(3), 330-333.
http://dx.doi.org/10.1578/AM.35.3.2009.330
---------- CHICAGO ----------
Pimper, L.E., Remis, M.I., Goodall, R.N.P., Baker, C.S. "Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina" . Aquatic Mammals 35, no. 3 (2009) : 330-333.
http://dx.doi.org/10.1578/AM.35.3.2009.330
---------- MLA ----------
Pimper, L.E., Remis, M.I., Goodall, R.N.P., Baker, C.S. "Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina" . Aquatic Mammals, vol. 35, no. 3, 2009, pp. 330-333.
http://dx.doi.org/10.1578/AM.35.3.2009.330
---------- VANCOUVER ----------
Pimper, L.E., Remis, M.I., Goodall, R.N.P., Baker, C.S. Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina. Aquatic Mamm. 2009;35(3):330-333.
http://dx.doi.org/10.1578/AM.35.3.2009.330