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Frydman, B.; Frydman, R.B.; de Los Santos, C.; Garrido, D.A.; Goldemberg, S.H.; Algranati, I.D. "Putrescine distribution in Escherichia coli studied in vivo by 13C nuclear magnetic resonance" (1984) BBA - Molecular Cell Research. 805(4):337-344
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Abstract:

In order to study the intracellular polyamine distribution in Escherichia coli, 13C-NMR spectra of [1,4-13C]putrescine were obtained after addition of the latter to intact bacteria. The 13C-enriched methylene signal underwent line broadening. When the cells were centrifuged after 90 min the cell-bound putrescine peak had a linewidth of 23 Hz, while the supernatant liquid showed an unbound putrescine signal with a linewidth smaller than 1 Hz. By using 13C-enriched internal standards it could be shown that the linewidening was not due to the heterogeneity of the medium or to an in vivo paramagnetic effect. Cell-bound putrescine was liberated by addition of trichloroacetic acid and was therefore non-covalently linked to macromolecular cell structures. Cell-bound [13C]putrescine could be displaced by addition of an excess of [12C]putrescine. When samples of membranes, soluble protein, DNA, tRNA and ribosomes from E. coli were incubated with [1,4-13C]putrescine, strong binding was detected only in the ribosomal and membrane fractions. The ribosome-putrescine complex showed properties similar to those determined with the intact cells. By measuring the nuclear Overhauser enhancements η, it was possible to estimate that only about 50% of the polyamine was linked to the macromolecules. Determination of the T1 values of free and ribosomal-bound putrescine allowed the calculation of a correlation time, τc = 4·10-7 s for the latter. T1 and τc value for the ribosome-putrescine complex were those expected for a motional regime of slowly tumbling molecules. © 1984.

Registro:

Documento: Artículo
Título:Putrescine distribution in Escherichia coli studied in vivo by 13C nuclear magnetic resonance
Autor:Frydman, B.; Frydman, R.B.; de Los Santos, C.; Garrido, D.A.; Goldemberg, S.H.; Algranati, I.D.
Filiación:Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín, 956 Buenos Aires, Argentina
Instituto de Investigaciones Bioquímicas 'Fundacion Campomar', Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Antonio Machado, 151 Buenos Aires, Argentina
Palabras clave:(E. coli); 13C-NMR; Putrescine distribution; bacterial DNA; carbon; putrescine; transfer RNA; article; Escherichia coli; methodology; nuclear magnetic resonance spectroscopy; ribosome; Carbon Isotopes; DNA, Bacterial; Escherichia coli; Magnetic Resonance Spectroscopy; Putrescine; Ribosomes; RNA, Transfer
Año:1984
Volumen:805
Número:4
Página de inicio:337
Página de fin:344
DOI: http://dx.doi.org/10.1016/0167-4889(84)90016-8
Título revista:BBA - Molecular Cell Research
Título revista abreviado:Biochim. Biophys. Acta Mol. Cell Res.
ISSN:01674889
CODEN:BAMRD
CAS:carbon, 7440-44-0; putrescine, 110-60-1, 333-93-7; transfer RNA, 9014-25-9; Carbon Isotopes; DNA, Bacterial; Putrescine, 110-60-1; RNA, Transfer, 9014-25-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v805_n4_p337_Frydman

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Citas:

---------- APA ----------
Frydman, B., Frydman, R.B., de Los Santos, C., Garrido, D.A., Goldemberg, S.H. & Algranati, I.D. (1984) . Putrescine distribution in Escherichia coli studied in vivo by 13C nuclear magnetic resonance. BBA - Molecular Cell Research, 805(4), 337-344.
http://dx.doi.org/10.1016/0167-4889(84)90016-8
---------- CHICAGO ----------
Frydman, B., Frydman, R.B., de Los Santos, C., Garrido, D.A., Goldemberg, S.H., Algranati, I.D. "Putrescine distribution in Escherichia coli studied in vivo by 13C nuclear magnetic resonance" . BBA - Molecular Cell Research 805, no. 4 (1984) : 337-344.
http://dx.doi.org/10.1016/0167-4889(84)90016-8
---------- MLA ----------
Frydman, B., Frydman, R.B., de Los Santos, C., Garrido, D.A., Goldemberg, S.H., Algranati, I.D. "Putrescine distribution in Escherichia coli studied in vivo by 13C nuclear magnetic resonance" . BBA - Molecular Cell Research, vol. 805, no. 4, 1984, pp. 337-344.
http://dx.doi.org/10.1016/0167-4889(84)90016-8
---------- VANCOUVER ----------
Frydman, B., Frydman, R.B., de Los Santos, C., Garrido, D.A., Goldemberg, S.H., Algranati, I.D. Putrescine distribution in Escherichia coli studied in vivo by 13C nuclear magnetic resonance. Biochim. Biophys. Acta Mol. Cell Res. 1984;805(4):337-344.
http://dx.doi.org/10.1016/0167-4889(84)90016-8