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Abstract:

Apoptosis of thymic cells induced by glucocorticoids (GC) and T-cell receptor (TCR) engagement are mutually antagonistic. We demonstrate that cAMP enhances GC and antagonizes TCR (anti-CD3) apoptosis on the same cell (DO-11.10 and 2B4.11 T-cell hybridomas). We analyzed the activity of several transcription factors in this cAMP dual, stimulus-dependent, regulatory action. Anti-CD3 increases kB-activity which is inhibited by CPTcAMP or dexamethasone (DEX), supporting the proapoptotic role of NFkB on TCR-induced apoptosis. Anti-CD3 not only increases kB- but diminishes GC response element (GRE)-activity induced by DEX, suggesting that TCR-mediated blockade of GC-induced apoptosis involves not only the proposed antiapoptotic action of NF-kB on GC, but also the inhibition of GRE-regulated proapoptotic genes. To test the involvement of CRE-driven transcription in the cAMP dual apoptotic regulation, cells were transfected with a CRE decoy DNA oligomer. Blockade of CRE transactivation with decoy targeting of CRE completely blocked the protection of TCR-induced apoptosis by cAMP, while it did not modify the enhancement by cAMP on GC-induced apoptosis. We show that CRE-binding factors have a definite role in T-cell apoptosis: they are involved in cAMP protection of TCR- but not in cAMP potentiation of GC-induced apoptosis. © 2002 Elsevier Science B.V. All rights reserved.

Registro:

Documento: Artículo
Título:CRE-Mediated transcriptional activation is involved in cAMP protection of T-cell receptor-induced apoptosis but not in cAMP potentiation of glucocorticoid-mediated programmed cell death
Autor:Igaz, L.M.; Refojo, D.; Costas, M.A.; Holsboer, F.; Arzt, E.
Filiación:Laboratorio de Fisiologı́a y Biologı́a Molecular, Departamento de Biologı́a, FCEN, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, 1428 Buenos Aires, Argentina
Max-Planck Institute of Psychiatry, Kraepelinstrasse 2-10, 80804 Munich, Germany
Palabras clave:Apoptosis; CAMP responsive element binding protein; Cyclic AMP; Glucocorticoids; T cell receptor; bucladesine; CD3 antigen; cyclic AMP; cyclic AMP responsive element binding protein; dexamethasone; DNA; glucocorticoid; immunoglobulin enhancer binding protein; monoclonal antibody CD3; oligomer; T lymphocyte receptor; animal cell; apoptosis; article; controlled study; cyclic AMP responsive element; enzyme activity; enzyme inhibition; genetic transfection; hybridoma cell culture; mouse; nonhuman; priority journal; regulatory mechanism; T lymphocyte; transcription initiation; Animals; Apoptosis; Cell Line; Cyclic AMP; Cyclic AMP Response Element-Binding Protein; Flow Cytometry; Glucocorticoids; Hybridomas; Mice; Receptors, Antigen, T-Cell; Response Elements; T-Lymphocytes; Transcription, Genetic; Transfection
Año:2001
Volumen:1542
Número:1-3
Página de inicio:139
Página de fin:148
DOI: http://dx.doi.org/10.1016/S0167-4889(01)00174-4
Título revista:Biochimica et Biophysica Acta - Molecular Cell Research
Título revista abreviado:Biochim. Biophys. Acta Mol. Cell Res.
ISSN:01674889
CAS:Cyclic AMP Response Element-Binding Protein; Cyclic AMP, 60-92-4; Glucocorticoids; Receptors, Antigen, T-Cell
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v1542_n1-3_p139_Igaz

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Citas:

---------- APA ----------
Igaz, L.M., Refojo, D., Costas, M.A., Holsboer, F. & Arzt, E. (2001) . CRE-Mediated transcriptional activation is involved in cAMP protection of T-cell receptor-induced apoptosis but not in cAMP potentiation of glucocorticoid-mediated programmed cell death. Biochimica et Biophysica Acta - Molecular Cell Research, 1542(1-3), 139-148.
http://dx.doi.org/10.1016/S0167-4889(01)00174-4
---------- CHICAGO ----------
Igaz, L.M., Refojo, D., Costas, M.A., Holsboer, F., Arzt, E. "CRE-Mediated transcriptional activation is involved in cAMP protection of T-cell receptor-induced apoptosis but not in cAMP potentiation of glucocorticoid-mediated programmed cell death" . Biochimica et Biophysica Acta - Molecular Cell Research 1542, no. 1-3 (2001) : 139-148.
http://dx.doi.org/10.1016/S0167-4889(01)00174-4
---------- MLA ----------
Igaz, L.M., Refojo, D., Costas, M.A., Holsboer, F., Arzt, E. "CRE-Mediated transcriptional activation is involved in cAMP protection of T-cell receptor-induced apoptosis but not in cAMP potentiation of glucocorticoid-mediated programmed cell death" . Biochimica et Biophysica Acta - Molecular Cell Research, vol. 1542, no. 1-3, 2001, pp. 139-148.
http://dx.doi.org/10.1016/S0167-4889(01)00174-4
---------- VANCOUVER ----------
Igaz, L.M., Refojo, D., Costas, M.A., Holsboer, F., Arzt, E. CRE-Mediated transcriptional activation is involved in cAMP protection of T-cell receptor-induced apoptosis but not in cAMP potentiation of glucocorticoid-mediated programmed cell death. Biochim. Biophys. Acta Mol. Cell Res. 2001;1542(1-3):139-148.
http://dx.doi.org/10.1016/S0167-4889(01)00174-4