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Abstract:

The cellular resistance to tumor necrosis factor (TNF) of most cell types has been attributed to both a protective pathway induced by this cytokine and the preexistence of protective factors in the target cell. NF-κB has been postulated as one of the principal factors involved in antiapoptotic gene expression control on TNF-resistant cells. We have previously shown that glucocorticoids protect the naturally TNF-sensitive L-929 cells from apoptosis. Here we analyze the role of NF-κB and glucocorticoids on TNF-induced apoptosis in L-929 cells. We found that inhibition of NF-κB enhanced the sensitivity to TNF-induced apoptosis. Glucocorticoids inhibited NF-κB transactivation via IκB induction. Moreover, glucocorticoids protected from TNF-induced apoptosis even when NF-κB activity was inhibited by stable or transient expression of the superrepressor IκB. These results demonstrate that although glucocorticoids inhibit NF-κB transactivation in these cells, this is not required for their protection from TNF-induced apoptosis. (C) 2000 Elsevier Science B.V.

Registro:

Documento: Artículo
Título:Transrepression of NF-κB is not required for glucocorticoid-mediated protection of TNF-α-induced apoptosis on fibroblasts
Autor:Costas, M.A.; Müller Igaz, L.; Holsboer, F.; Arzt, E.
Filiación:Laboratorio de Fisiología y Biología Molecular, Departamento de Ciencias Biológicas, Pabellón II, C1428EHA Buenos Aires, Argentina
Max-Planck Institute of Psychiatry, Kraepelinstrasse 210, 80804 Munich, Germany
Palabras clave:Apoptosis; Glucocorticoid; Glucocorticoid receptor; Nuclear factor-κB; Tumor necrosis factor; beta galactosidase; dexamethasone; glucocorticoid; glucocorticoid receptor; I kappa B; immunoglobulin enhancer binding protein; luciferase; messenger RNA; tumor necrosis factor alpha; tumor necrosis factor alpha receptor; DNA binding protein; glucocorticoid; glucocorticoid receptor; I kappa B; immunoglobulin enhancer binding protein; NF kappaB inhibitor alpha; NF-kappaB inhibitor alpha; tumor necrosis factor alpha; animal cell; apoptosis; article; cell protection; controlled study; cytotoxicity; fibroblast; gene expression regulation; gene repression; mouse; nonhuman; priority journal; protein expression; suppressor cell; target cell destruction; transactivation; animal; biosynthesis; cell line; drug antagonism; genetic transfection; metabolism; Animals; Apoptosis; Cell Line; DNA-Binding Proteins; Fibroblasts; Glucocorticoids; I-kappa B Proteins; Mice; NF-kappa B; Receptors, Glucocorticoid; Transfection; Tumor Necrosis Factor-alpha
Año:2000
Volumen:1499
Número:1-2
Página de inicio:122
Página de fin:129
DOI: http://dx.doi.org/10.1016/S0167-4889(00)00113-0
Título revista:Biochimica et Biophysica Acta - Molecular Cell Research
Título revista abreviado:Biochim. Biophys. Acta Mol. Cell Res.
ISSN:01674889
CODEN:BAMRD
CAS:DNA-Binding Proteins; Glucocorticoids; I-kappa B Proteins; NF-kappa B; NF-kappaB inhibitor alpha, 139874-52-5; Receptors, Glucocorticoid; Tumor Necrosis Factor-alpha
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_01674889_v1499_n1-2_p122_Costas.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v1499_n1-2_p122_Costas

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Citas:

---------- APA ----------
Costas, M.A., Müller Igaz, L., Holsboer, F. & Arzt, E. (2000) . Transrepression of NF-κB is not required for glucocorticoid-mediated protection of TNF-α-induced apoptosis on fibroblasts. Biochimica et Biophysica Acta - Molecular Cell Research, 1499(1-2), 122-129.
http://dx.doi.org/10.1016/S0167-4889(00)00113-0
---------- CHICAGO ----------
Costas, M.A., Müller Igaz, L., Holsboer, F., Arzt, E. "Transrepression of NF-κB is not required for glucocorticoid-mediated protection of TNF-α-induced apoptosis on fibroblasts" . Biochimica et Biophysica Acta - Molecular Cell Research 1499, no. 1-2 (2000) : 122-129.
http://dx.doi.org/10.1016/S0167-4889(00)00113-0
---------- MLA ----------
Costas, M.A., Müller Igaz, L., Holsboer, F., Arzt, E. "Transrepression of NF-κB is not required for glucocorticoid-mediated protection of TNF-α-induced apoptosis on fibroblasts" . Biochimica et Biophysica Acta - Molecular Cell Research, vol. 1499, no. 1-2, 2000, pp. 122-129.
http://dx.doi.org/10.1016/S0167-4889(00)00113-0
---------- VANCOUVER ----------
Costas, M.A., Müller Igaz, L., Holsboer, F., Arzt, E. Transrepression of NF-κB is not required for glucocorticoid-mediated protection of TNF-α-induced apoptosis on fibroblasts. Biochim. Biophys. Acta Mol. Cell Res. 2000;1499(1-2):122-129.
http://dx.doi.org/10.1016/S0167-4889(00)00113-0