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Abstract:

Phorbol esters stimulate cyclic adenosine 3′,5′-monophosphate (cAMP) accumulation in hamster spermatozoa under conditions for in vitro capacitation. The 20-50-fold elevation of cAMP levels induced by 1 μM phorbol 12-myristate 13-acetate (PMA) in spermatozoa depends on the presence of sodium bicarbonate in the medium (ED50: 15 mM) and it is independent of extracellular pH. Sodium bicarbonate stimulates adenylate cyclase activity in membrane preparations by 4-fold (ED50: 40 mM). After sulubilization, the bicarbonate-sensitive moiety elutes as a single peak of 55 kDa in a gel filtration column. Blockers of bicarbonate chloride antiporters diisothiocyanate stilbene 2,2′-disulfonic acid (DIDS) or acetamido 4′-isothiocyanate stilbene 2,2′-disulfonic acid (SITS) inhibit the bicarbonate dependent PMA effect on cAMP in living spermatozoa (ED50: 100 μM). Maximal (85%) inhibition in cAMP accumulation is observed at 1 mM. Motility is inhibited only at high concentrations of the blockers. Pretreatment of living cells with 1 mM DIDS does not affect membrane adenylate cyclase activity which remains responsive to bicarbonate. These results suggest that controlled transport of bicarbonate through the sperm plasma membrane could be associated to the regulation of cAMP synthesis. © 1990.

Registro:

Documento: Artículo
Título:Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa
Autor:Visconti, P.E.; Muschietti, J.P.; Flawia, M.M.; Tezon, J.G.
Filiación:Instituto de Biologia y Medicina Experimental (IBYME), Buenos Aires, Argentina
Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI-CONICET), Facultad de Ciencias Exactas y Naturales (UBA), Buenos Aires, Argentina
Palabras clave:(Hamster spermatozoa); Adenylate cyclase; Bicarbonate; cyclic AMP; Phorbol myristate acetate; adenylate cyclase; bicarbonate; cyclic amp; phorbol ester; animal cell; article; cell membrane; hamster; nonhuman; priority journal; spermatozoon; 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; Adenylate Cyclase; Animal; Bicarbonates; Biological Transport; Cyclic AMP; Enzyme Activation; Epididymis; Hamsters; Hydrogen-Ion Concentration; In Vitro; Male; Phorbol 12,13-Dibutyrate; Sodium; Sodium Bicarbonate; Sperm Capacitation; Spermatozoa; Support, Non-U.S. Gov't; Animalia; Cricetinae
Año:1990
Volumen:1054
Número:2
Página de inicio:231
Página de fin:236
DOI: http://dx.doi.org/10.1016/0167-4889(90)90246-A
Título revista:BBA - Molecular Cell Research
Título revista abreviado:Biochim. Biophys. Acta Mol. Cell Res.
ISSN:01674889
CODEN:BAMRD
CAS:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid, 53005-05-3; 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid, 27816-59-7; Adenylate Cyclase, EC 4.6.1.1; Bicarbonates; Cyclic AMP, 60-92-4; Phorbol 12,13-Dibutyrate, 37558-16-0; Sodium Bicarbonate, 144-55-8; Sodium, 7440-23-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v1054_n2_p231_Visconti

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Citas:

---------- APA ----------
Visconti, P.E., Muschietti, J.P., Flawia, M.M. & Tezon, J.G. (1990) . Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa. BBA - Molecular Cell Research, 1054(2), 231-236.
http://dx.doi.org/10.1016/0167-4889(90)90246-A
---------- CHICAGO ----------
Visconti, P.E., Muschietti, J.P., Flawia, M.M., Tezon, J.G. "Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa" . BBA - Molecular Cell Research 1054, no. 2 (1990) : 231-236.
http://dx.doi.org/10.1016/0167-4889(90)90246-A
---------- MLA ----------
Visconti, P.E., Muschietti, J.P., Flawia, M.M., Tezon, J.G. "Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa" . BBA - Molecular Cell Research, vol. 1054, no. 2, 1990, pp. 231-236.
http://dx.doi.org/10.1016/0167-4889(90)90246-A
---------- VANCOUVER ----------
Visconti, P.E., Muschietti, J.P., Flawia, M.M., Tezon, J.G. Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa. Biochim. Biophys. Acta Mol. Cell Res. 1990;1054(2):231-236.
http://dx.doi.org/10.1016/0167-4889(90)90246-A