Moschen, S.; Di Rienzo, J.A.; Higgins, J.; Tohge, T.; Watanabe, M.; González, S.; Rivarola, M.; García-García, F.; Dopazo, J.; Hopp, H.E.; Hoefgen, R.; Fernie, A.R.; Paniego, N.; Fernández, P.; Heinz, R.A."Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.)" (2017) Plant Molecular Biology. 94(4-5):549-564
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Key message: By integration of transcriptional and metabolic profiles we identified pathways and hubs transcription factors regulated during drought conditions in sunflower, useful for applications in molecular and/or biotechnological breeding. Abstract: Drought is one of the most important environmental stresses that effects crop productivity in many agricultural regions. Sunflower is tolerant to drought conditions but the mechanisms involved in this tolerance remain unclear at the molecular level. The aim of this study was to characterize and integrate transcriptional and metabolic pathways related to drought stress in sunflower plants, by using a system biology approach. Our results showed a delay in plant senescence with an increase in the expression level of photosynthesis related genes as well as higher levels of sugars, osmoprotectant amino acids and ionic nutrients under drought conditions. In addition, we identified transcription factors that were upregulated during drought conditions and that may act as hubs in the transcriptional network. Many of these transcription factors belong to families implicated in the drought response in model species. The integration of transcriptomic and metabolomic data in this study, together with physiological measurements, has improved our understanding of the biological responses during droughts and contributes to elucidate the molecular mechanisms involved under this environmental condition. These findings will provide useful biotechnological tools to improve stress tolerance while maintaining crop yield under restricted water availability. © 2017, Springer Science+Business Media B.V.


Documento: Artículo
Título:Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.)
Autor:Moschen, S.; Di Rienzo, J.A.; Higgins, J.; Tohge, T.; Watanabe, M.; González, S.; Rivarola, M.; García-García, F.; Dopazo, J.; Hopp, H.E.; Hoefgen, R.; Fernie, A.R.; Paniego, N.; Fernández, P.; Heinz, R.A.
Filiación:Instituto de Biotecnología, Centro de Investigaciones en Ciencias Agronómicas y Veterinarias, Instituto Nacional de Tecnología Agropecuaria, Hurlingham, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, Argentina
Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba, Córdoba, Argentina
Earlham Institute, Norwich Research Park, Norwich, NR4 7UZ, United Kingdom
Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany
Computational Genomics Department, Centro de Investigación Príncipe Felipe. Functional Genomics Node (INB-ELIXIR-es). Bioinformatics in Rare Diseases (BiER), Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Valencia, 46012, Spain
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina
Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, San Martín, Buenos Aires, Argentina
Palabras clave:Data integration; Drought; Helianthus annuus L; Metabolomics; Sunflower; Transcriptomics; chlorophyll; plant protein; plant RNA; transcription factor; water; gene expression regulation; genetics; metabolism; physiological stress; physiology; plant leaf; protein microarray; sunflower; Chlorophyll; Gene Expression Regulation, Plant; Helianthus; Plant Leaves; Plant Proteins; Protein Array Analysis; RNA, Plant; Stress, Physiological; Transcription Factors; Water
Página de inicio:549
Página de fin:564
Título revista:Plant Molecular Biology
Título revista abreviado:Plant. Mol. Biol.
CAS:chlorophyll, 1406-65-1, 15611-43-5; water, 7732-18-5; Chlorophyll; Plant Proteins; RNA, Plant; Transcription Factors; Water


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---------- APA ----------
Moschen, S., Di Rienzo, J.A., Higgins, J., Tohge, T., Watanabe, M., González, S., Rivarola, M.,..., Heinz, R.A. (2017) . Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.). Plant Molecular Biology, 94(4-5), 549-564.
---------- CHICAGO ----------
Moschen, S., Di Rienzo, J.A., Higgins, J., Tohge, T., Watanabe, M., González, S., et al. "Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.)" . Plant Molecular Biology 94, no. 4-5 (2017) : 549-564.
---------- MLA ----------
Moschen, S., Di Rienzo, J.A., Higgins, J., Tohge, T., Watanabe, M., González, S., et al. "Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.)" . Plant Molecular Biology, vol. 94, no. 4-5, 2017, pp. 549-564.
---------- VANCOUVER ----------
Moschen, S., Di Rienzo, J.A., Higgins, J., Tohge, T., Watanabe, M., González, S., et al. Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.). Plant. Mol. Biol. 2017;94(4-5):549-564.